Experiment: m.b. Cephalothin 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0028 and Dshi_0029 are separated by 85 nucleotides Dshi_0029 and Dshi_0030 are separated by 11 nucleotides Dshi_0030 and Dshi_0031 are separated by 35 nucleotides
Dshi_0028: Dshi_0028 - A/G-specific adenine glycosylase (RefSeq), at 31,815 to 32,909
_0028
Dshi_0029: Dshi_0029 - protein of unknown function DUF1159 (RefSeq), at 32,995 to 33,507
_0029
Dshi_0030: Dshi_0030 - thiol-disulfide oxidoreductase D, putative (RefSeq), at 33,519 to 34,190
_0030
Dshi_0031: Dshi_0031 - tetraacyldisaccharide 4'-kinase (RefSeq), at 34,226 to 35,239
_0031
Position (kb) 33
34
35 Strain fitness (log2 ratio) -2
-1
0
1 at 32.520 kb on - strand, within Dshi_0028 at 32.629 kb on + strand, within Dshi_0028 at 32.678 kb on + strand, within Dshi_0028 at 32.949 kb on + strand at 32.956 kb on + strand at 32.961 kb on + strand at 33.586 kb on - strand at 33.601 kb on - strand, within Dshi_0030 at 33.717 kb on + strand, within Dshi_0030 at 33.759 kb on - strand, within Dshi_0030 at 33.853 kb on + strand, within Dshi_0030 at 33.854 kb on + strand, within Dshi_0030 at 33.863 kb on - strand, within Dshi_0030 at 34.060 kb on + strand, within Dshi_0030 at 34.071 kb on - strand, within Dshi_0030
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cephalothin 0.0001 mg/ml remove 32,520 - Dshi_0028 0.64 +0.4 32,629 + Dshi_0028 0.74 -1.4 32,678 + Dshi_0028 0.79 -0.2 32,949 + -1.1 32,956 + +0.6 32,961 + +0.0 33,586 - -2.3 33,601 - Dshi_0030 0.12 -2.0 33,717 + Dshi_0030 0.29 -0.6 33,759 - Dshi_0030 0.36 -1.2 33,853 + Dshi_0030 0.50 -1.6 33,854 + Dshi_0030 0.50 -1.9 33,863 - Dshi_0030 0.51 -2.6 34,060 + Dshi_0030 0.81 -1.3 34,071 - Dshi_0030 0.82 -1.1
Or see this region's nucleotide sequence