Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1810 and SO1811 overlap by 4 nucleotides SO1811 and mdeA are separated by 284 nucleotides
SO1810: SO1810 - hypothetical ATPase (NCBI ptt file), at 1,899,256 to 1,900,713
SO1810
SO1811: SO1811 - conserved hypothetical protein (NCBI ptt file), at 1,900,710 to 1,901,915
SO1811
SO1812: mdeA - Methionine gamma-lyase (EC 4.4.1.11) (from data) , at 1,902,200 to 1,903,393
mdeA
Position (kb)
1900
1901
1902 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1899.756 kb on - strand, within SO1810 at 1900.005 kb on + strand, within SO1810 at 1900.034 kb on + strand, within SO1810 at 1900.042 kb on - strand, within SO1810 at 1900.042 kb on - strand, within SO1810 at 1900.219 kb on + strand, within SO1810 at 1900.503 kb on - strand, within SO1810 at 1900.528 kb on - strand, within SO1810 at 1900.533 kb on - strand, within SO1810 at 1900.557 kb on - strand, within SO1810 at 1900.558 kb on + strand, within SO1810 at 1900.652 kb on + strand at 1900.722 kb on - strand at 1900.859 kb on + strand, within SO1811 at 1901.055 kb on - strand, within SO1811 at 1901.086 kb on - strand, within SO1811 at 1901.123 kb on - strand, within SO1811 at 1901.218 kb on + strand, within SO1811 at 1901.218 kb on + strand, within SO1811 at 1901.218 kb on + strand, within SO1811 at 1901.276 kb on + strand, within SO1811 at 1901.538 kb on + strand, within SO1811 at 1901.559 kb on - strand, within SO1811 at 1901.560 kb on - strand, within SO1811 at 1901.616 kb on + strand, within SO1811 at 1901.679 kb on + strand, within SO1811 at 1901.701 kb on - strand, within SO1811 at 1901.706 kb on + strand, within SO1811 at 1902.066 kb on - strand at 1902.070 kb on + strand at 1902.331 kb on + strand, within mdeA at 1902.373 kb on - strand, within mdeA at 1902.373 kb on - strand, within mdeA at 1902.373 kb on - strand, within mdeA at 1902.407 kb on - strand, within mdeA at 1902.536 kb on + strand, within mdeA at 1902.536 kb on + strand, within mdeA at 1902.801 kb on + strand, within mdeA at 1902.820 kb on - strand, within mdeA at 1902.827 kb on + strand, within mdeA at 1902.887 kb on - strand, within mdeA
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 1,899,756 - SO1810 0.34 +0.5 1,900,005 + SO1810 0.51 -0.1 1,900,034 + SO1810 0.53 +2.6 1,900,042 - SO1810 0.54 -0.5 1,900,042 - SO1810 0.54 -0.5 1,900,219 + SO1810 0.66 -0.0 1,900,503 - SO1810 0.86 -1.0 1,900,528 - SO1810 0.87 -0.2 1,900,533 - SO1810 0.88 -0.6 1,900,557 - SO1810 0.89 +0.1 1,900,558 + SO1810 0.89 -0.4 1,900,652 + +0.7 1,900,722 - +0.2 1,900,859 + SO1811 0.12 -0.3 1,901,055 - SO1811 0.29 +0.5 1,901,086 - SO1811 0.31 -0.1 1,901,123 - SO1811 0.34 -1.2 1,901,218 + SO1811 0.42 -0.4 1,901,218 + SO1811 0.42 +1.4 1,901,218 + SO1811 0.42 -0.8 1,901,276 + SO1811 0.47 -0.7 1,901,538 + SO1811 0.69 -0.9 1,901,559 - SO1811 0.70 -0.3 1,901,560 - SO1811 0.70 -0.6 1,901,616 + SO1811 0.75 -0.2 1,901,679 + SO1811 0.80 -1.8 1,901,701 - SO1811 0.82 +0.2 1,901,706 + SO1811 0.83 -1.5 1,902,066 - -0.2 1,902,070 + +0.4 1,902,331 + mdeA SO1812 0.11 -0.8 1,902,373 - mdeA SO1812 0.14 -0.3 1,902,373 - mdeA SO1812 0.14 -0.2 1,902,373 - mdeA SO1812 0.14 -0.1 1,902,407 - mdeA SO1812 0.17 +0.1 1,902,536 + mdeA SO1812 0.28 -0.1 1,902,536 + mdeA SO1812 0.28 -0.0 1,902,801 + mdeA SO1812 0.50 +0.0 1,902,820 - mdeA SO1812 0.52 +0.7 1,902,827 + mdeA SO1812 0.53 -0.1 1,902,887 - mdeA SO1812 0.58 +1.3
Or see this region's nucleotide sequence