Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuC and leuB overlap by 1 nucleotides leuB and leuA are separated by 51 nucleotides
SO4234: leuC - 3-isopropylmalate dehydratase, large subunit (NCBI ptt file), at 4,411,047 to 4,412,471
leuC
SO4235: leuB - 3-isopropylmalate dehydrogenase (NCBI ptt file), at 4,412,471 to 4,413,565
leuB
SO4236: leuA - 2-isopropylmalate synthase (NCBI ptt file), at 4,413,617 to 4,415,185
leuA
Position (kb)
4412
4413
4414 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 4411.471 kb on + strand, within leuC at 4411.621 kb on - strand, within leuC at 4411.624 kb on - strand, within leuC at 4411.626 kb on + strand, within leuC at 4411.651 kb on - strand, within leuC at 4411.680 kb on + strand, within leuC at 4411.715 kb on + strand, within leuC at 4411.768 kb on - strand, within leuC at 4411.815 kb on - strand, within leuC at 4411.928 kb on - strand, within leuC at 4412.105 kb on + strand, within leuC at 4412.113 kb on - strand, within leuC at 4412.221 kb on - strand, within leuC at 4412.336 kb on - strand at 4412.358 kb on + strand at 4412.484 kb on - strand at 4412.555 kb on - strand at 4412.608 kb on - strand, within leuB at 4412.629 kb on + strand, within leuB at 4412.629 kb on + strand, within leuB at 4412.637 kb on - strand, within leuB at 4412.784 kb on + strand, within leuB at 4412.830 kb on + strand, within leuB at 4412.838 kb on - strand, within leuB at 4412.838 kb on - strand, within leuB at 4412.847 kb on + strand, within leuB at 4412.848 kb on + strand, within leuB at 4412.855 kb on - strand, within leuB at 4412.857 kb on + strand, within leuB at 4412.895 kb on + strand, within leuB at 4412.957 kb on + strand, within leuB at 4412.957 kb on + strand, within leuB at 4412.965 kb on - strand, within leuB at 4412.979 kb on + strand, within leuB at 4412.980 kb on - strand, within leuB at 4413.068 kb on + strand, within leuB at 4413.083 kb on + strand, within leuB at 4413.103 kb on + strand, within leuB at 4413.104 kb on + strand, within leuB at 4413.105 kb on + strand, within leuB at 4413.120 kb on + strand, within leuB at 4413.128 kb on - strand, within leuB at 4413.217 kb on + strand, within leuB at 4413.218 kb on + strand, within leuB at 4413.294 kb on - strand, within leuB at 4413.389 kb on - strand, within leuB at 4413.396 kb on - strand, within leuB at 4413.419 kb on + strand, within leuB at 4413.486 kb on + strand at 4413.524 kb on + strand at 4413.525 kb on - strand at 4413.579 kb on + strand at 4413.587 kb on - strand at 4413.587 kb on - strand at 4413.587 kb on - strand at 4413.600 kb on + strand at 4413.699 kb on - strand at 4413.813 kb on - strand, within leuA at 4413.839 kb on + strand, within leuA at 4413.902 kb on + strand, within leuA at 4413.932 kb on - strand, within leuA at 4414.040 kb on - strand, within leuA at 4414.077 kb on + strand, within leuA at 4414.100 kb on + strand, within leuA at 4414.200 kb on + strand, within leuA at 4414.227 kb on - strand, within leuA at 4414.266 kb on + strand, within leuA at 4414.293 kb on + strand, within leuA at 4414.311 kb on - strand, within leuA at 4414.311 kb on - strand, within leuA at 4414.472 kb on + strand, within leuA at 4414.486 kb on + strand, within leuA at 4414.486 kb on + strand, within leuA at 4414.494 kb on - strand, within leuA at 4414.494 kb on - strand, within leuA at 4414.534 kb on - strand, within leuA
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 4,411,471 + leuC SO4234 0.30 -4.0 4,411,621 - leuC SO4234 0.40 -2.0 4,411,624 - leuC SO4234 0.40 -2.4 4,411,626 + leuC SO4234 0.41 -3.3 4,411,651 - leuC SO4234 0.42 -4.0 4,411,680 + leuC SO4234 0.44 -4.2 4,411,715 + leuC SO4234 0.47 -2.8 4,411,768 - leuC SO4234 0.51 -2.5 4,411,815 - leuC SO4234 0.54 -3.6 4,411,928 - leuC SO4234 0.62 -2.3 4,412,105 + leuC SO4234 0.74 -3.0 4,412,113 - leuC SO4234 0.75 -3.9 4,412,221 - leuC SO4234 0.82 -2.0 4,412,336 - -3.4 4,412,358 + -2.0 4,412,484 - -3.9 4,412,555 - -2.9 4,412,608 - leuB SO4235 0.13 -3.9 4,412,629 + leuB SO4235 0.14 -4.4 4,412,629 + leuB SO4235 0.14 -2.3 4,412,637 - leuB SO4235 0.15 -4.2 4,412,784 + leuB SO4235 0.29 -1.8 4,412,830 + leuB SO4235 0.33 -2.6 4,412,838 - leuB SO4235 0.34 -4.9 4,412,838 - leuB SO4235 0.34 -2.1 4,412,847 + leuB SO4235 0.34 -3.1 4,412,848 + leuB SO4235 0.34 -4.1 4,412,855 - leuB SO4235 0.35 -3.7 4,412,857 + leuB SO4235 0.35 -0.9 4,412,895 + leuB SO4235 0.39 -3.5 4,412,957 + leuB SO4235 0.44 -4.0 4,412,957 + leuB SO4235 0.44 -1.6 4,412,965 - leuB SO4235 0.45 -4.2 4,412,979 + leuB SO4235 0.46 -4.4 4,412,980 - leuB SO4235 0.46 -3.9 4,413,068 + leuB SO4235 0.55 -4.8 4,413,083 + leuB SO4235 0.56 -1.9 4,413,103 + leuB SO4235 0.58 -4.2 4,413,104 + leuB SO4235 0.58 -4.1 4,413,105 + leuB SO4235 0.58 -4.2 4,413,120 + leuB SO4235 0.59 -4.3 4,413,128 - leuB SO4235 0.60 -3.3 4,413,217 + leuB SO4235 0.68 -2.7 4,413,218 + leuB SO4235 0.68 -4.6 4,413,294 - leuB SO4235 0.75 -4.1 4,413,389 - leuB SO4235 0.84 -5.6 4,413,396 - leuB SO4235 0.84 -4.8 4,413,419 + leuB SO4235 0.87 -1.1 4,413,486 + -3.6 4,413,524 + -4.0 4,413,525 - -2.4 4,413,579 + -4.2 4,413,587 - -2.0 4,413,587 - -0.6 4,413,587 - -4.1 4,413,600 + -3.7 4,413,699 - -4.4 4,413,813 - leuA SO4236 0.12 -2.6 4,413,839 + leuA SO4236 0.14 -6.3 4,413,902 + leuA SO4236 0.18 -1.9 4,413,932 - leuA SO4236 0.20 -4.0 4,414,040 - leuA SO4236 0.27 -3.2 4,414,077 + leuA SO4236 0.29 -4.4 4,414,100 + leuA SO4236 0.31 -3.4 4,414,200 + leuA SO4236 0.37 -3.1 4,414,227 - leuA SO4236 0.39 -4.3 4,414,266 + leuA SO4236 0.41 -2.8 4,414,293 + leuA SO4236 0.43 -2.9 4,414,311 - leuA SO4236 0.44 -2.5 4,414,311 - leuA SO4236 0.44 -0.1 4,414,472 + leuA SO4236 0.54 -4.0 4,414,486 + leuA SO4236 0.55 -3.8 4,414,486 + leuA SO4236 0.55 -4.0 4,414,494 - leuA SO4236 0.56 -4.6 4,414,494 - leuA SO4236 0.56 -1.5 4,414,534 - leuA SO4236 0.58 -3.8
Or see this region's nucleotide sequence