Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3172c

Experiment: 7H9 with 2-bromo-2-nitro-1,3-propanediol 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3171c and Rv3172c are separated by 136 nucleotidesRv3172c and Rv3173c are separated by 78 nucleotidesRv3173c and Rv3174 are separated by 92 nucleotides Rv3171c: Rv3171c - Possible non-heme haloperoxidase Hpx, at 3,539,846 to 3,540,745 Rv3171c Rv3172c: Rv3172c - Hypothetical protein, at 3,540,882 to 3,541,364 Rv3172c Rv3173c: Rv3173c - Probable transcriptional regulatory protein (probably TetR/AcrR-family), at 3,541,443 to 3,542,045 Rv3173c Rv3174: Rv3174 - Probable short-chain dehydrogenase/reductase, at 3,542,138 to 3,542,845 Rv3174 Position (kb) 3540 3541 3542Strain fitness (log2 ratio) -1 0 1 2at 3540.036 kb on - strand, within Rv3171cat 3540.540 kb on + strand, within Rv3171cat 3540.762 kb on - strandat 3540.915 kb on - strandat 3540.915 kb on - strandat 3540.980 kb on + strand, within Rv3172cat 3540.980 kb on + strand, within Rv3172cat 3541.081 kb on + strand, within Rv3172cat 3541.081 kb on + strand, within Rv3172cat 3541.081 kb on + strand, within Rv3172cat 3541.082 kb on - strand, within Rv3172cat 3541.162 kb on - strand, within Rv3172cat 3541.268 kb on - strand, within Rv3172cat 3541.268 kb on - strand, within Rv3172cat 3541.289 kb on - strand, within Rv3172cat 3541.291 kb on + strand, within Rv3172cat 3541.291 kb on + strand, within Rv3172cat 3541.291 kb on + strand, within Rv3172cat 3541.375 kb on + strandat 3541.375 kb on + strandat 3541.634 kb on - strand, within Rv3173cat 3541.866 kb on - strand, within Rv3173cat 3541.983 kb on - strand, within Rv3173cat 3541.983 kb on - strand, within Rv3173cat 3542.045 kb on + strandat 3542.069 kb on + strandat 3542.091 kb on - strandat 3542.202 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with 2-bromo-2-nitro-1,3-propanediol 0.1 mM
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3,540,036 - Rv3171c 0.21 +1.7
3,540,540 + Rv3171c 0.77 -1.1
3,540,762 - -0.8
3,540,915 - +0.7
3,540,915 - +0.1
3,540,980 + Rv3172c 0.20 -1.1
3,540,980 + Rv3172c 0.20 -1.5
3,541,081 + Rv3172c 0.41 -0.7
3,541,081 + Rv3172c 0.41 -1.3
3,541,081 + Rv3172c 0.41 -0.5
3,541,082 - Rv3172c 0.41 -0.3
3,541,162 - Rv3172c 0.58 +0.1
3,541,268 - Rv3172c 0.80 -1.3
3,541,268 - Rv3172c 0.80 +0.1
3,541,289 - Rv3172c 0.84 -0.5
3,541,291 + Rv3172c 0.85 -1.1
3,541,291 + Rv3172c 0.85 -0.0
3,541,291 + Rv3172c 0.85 -0.2
3,541,375 + -0.6
3,541,375 + -0.3
3,541,634 - Rv3173c 0.32 +0.8
3,541,866 - Rv3173c 0.70 +0.2
3,541,983 - Rv3173c 0.90 +2.0
3,541,983 - Rv3173c 0.90 +0.7
3,542,045 + -0.1
3,542,069 + +0.1
3,542,091 - +1.1
3,542,202 - +0.1

Or see this region's nucleotide sequence