Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2729c

Experiment: 7H9 with Spectinomycin 0.029 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2727c and Rv2728c overlap by 4 nucleotidesRv2728c and Rv2729c are separated by 108 nucleotidesRv2729c and Rv2730 are separated by 66 nucleotidesRv2730 and Rv2731 are separated by 7 nucleotides Rv2727c: Rv2727c - Probable tRNA delta(2)-isopentenylpyrophosphate transferase MiaA (IPP transferase) (isopentenyl-diphosphate:tRNA isopentenyltransferase) (iptase) (IPPT), at 3,039,825 to 3,040,769 Rv2727c Rv2728c: Rv2728c - Conserved alanine rich protein, at 3,040,766 to 3,041,461 Rv2728c Rv2729c: Rv2729c - Probable conserved integral membrane alanine valine and leucine rich protein, at 3,041,570 to 3,042,475 Rv2729c Rv2730: Rv2730 - Hypothetical protein, at 3,042,542 to 3,043,018 Rv2730 Rv2731: Rv2731 - Conserved alanine and arginine rich protein, at 3,043,026 to 3,044,378 Rv2731 Position (kb) 3041 3042 3043Strain fitness (log2 ratio) -2 -1 0 1 2at 3040.819 kb on - strandat 3040.981 kb on - strand, within Rv2728cat 3041.031 kb on - strand, within Rv2728cat 3041.177 kb on - strand, within Rv2728cat 3041.196 kb on - strand, within Rv2728cat 3041.196 kb on - strand, within Rv2728cat 3041.473 kb on - strandat 3041.473 kb on - strandat 3041.473 kb on - strandat 3041.651 kb on + strandat 3041.651 kb on + strandat 3041.865 kb on + strand, within Rv2729cat 3041.865 kb on + strand, within Rv2729cat 3041.866 kb on - strand, within Rv2729cat 3041.943 kb on - strand, within Rv2729cat 3042.085 kb on + strand, within Rv2729cat 3042.366 kb on + strand, within Rv2729cat 3042.367 kb on - strand, within Rv2729cat 3042.367 kb on - strand, within Rv2729cat 3042.367 kb on - strand, within Rv2729cat 3042.411 kb on + strandat 3042.412 kb on - strandat 3042.475 kb on + strandat 3042.475 kb on + strandat 3042.476 kb on - strandat 3042.516 kb on + strandat 3042.517 kb on - strandat 3042.517 kb on - strandat 3042.529 kb on - strandat 3042.569 kb on - strandat 3042.569 kb on - strandat 3042.630 kb on + strand, within Rv2730at 3042.722 kb on + strand, within Rv2730at 3042.723 kb on - strand, within Rv2730at 3042.723 kb on - strand, within Rv2730at 3042.778 kb on + strand, within Rv2730at 3042.778 kb on + strand, within Rv2730at 3042.779 kb on - strand, within Rv2730at 3042.781 kb on + strand, within Rv2730at 3042.818 kb on - strand, within Rv2730at 3042.818 kb on - strand, within Rv2730at 3042.865 kb on - strand, within Rv2730at 3042.897 kb on + strand, within Rv2730at 3042.898 kb on - strand, within Rv2730at 3042.909 kb on + strand, within Rv2730at 3042.910 kb on - strand, within Rv2730at 3042.992 kb on - strandat 3043.012 kb on + strandat 3043.012 kb on + strandat 3043.012 kb on + strandat 3043.012 kb on + strandat 3043.013 kb on - strandat 3043.013 kb on - strandat 3043.013 kb on - strandat 3043.013 kb on - strandat 3043.013 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Spectinomycin 0.029 mM
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3,040,819 - +0.0
3,040,981 - Rv2728c 0.31 -0.3
3,041,031 - Rv2728c 0.38 +0.7
3,041,177 - Rv2728c 0.59 -0.1
3,041,196 - Rv2728c 0.62 +0.2
3,041,196 - Rv2728c 0.62 -0.1
3,041,473 - -1.4
3,041,473 - +0.0
3,041,473 - -0.4
3,041,651 + +1.3
3,041,651 + -0.1
3,041,865 + Rv2729c 0.33 -0.8
3,041,865 + Rv2729c 0.33 -0.9
3,041,866 - Rv2729c 0.33 -0.4
3,041,943 - Rv2729c 0.41 -1.6
3,042,085 + Rv2729c 0.57 -0.9
3,042,366 + Rv2729c 0.88 +0.3
3,042,367 - Rv2729c 0.88 -2.1
3,042,367 - Rv2729c 0.88 -0.7
3,042,367 - Rv2729c 0.88 -0.9
3,042,411 + +0.6
3,042,412 - -0.2
3,042,475 + +0.9
3,042,475 + +0.3
3,042,476 - -0.1
3,042,516 + -0.6
3,042,517 - +0.0
3,042,517 - +0.3
3,042,529 - -1.0
3,042,569 - -0.7
3,042,569 - -1.8
3,042,630 + Rv2730 0.18 -1.1
3,042,722 + Rv2730 0.38 +0.2
3,042,723 - Rv2730 0.38 +0.5
3,042,723 - Rv2730 0.38 +0.7
3,042,778 + Rv2730 0.49 -0.7
3,042,778 + Rv2730 0.49 -0.1
3,042,779 - Rv2730 0.50 +0.4
3,042,781 + Rv2730 0.50 -0.8
3,042,818 - Rv2730 0.58 -0.8
3,042,818 - Rv2730 0.58 +0.1
3,042,865 - Rv2730 0.68 +0.2
3,042,897 + Rv2730 0.74 -0.2
3,042,898 - Rv2730 0.75 -0.1
3,042,909 + Rv2730 0.77 +1.0
3,042,910 - Rv2730 0.77 +0.1
3,042,992 - +1.9
3,043,012 + +0.3
3,043,012 + -0.5
3,043,012 + +0.2
3,043,012 + +0.9
3,043,013 - -0.2
3,043,013 - -0.6
3,043,013 - -0.4
3,043,013 - -0.1
3,043,013 - -1.0

Or see this region's nucleotide sequence