Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0100

Experiment: no stress control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0099 and Rv0100 are separated by 4 nucleotidesRv0100 and Rv0101 overlap by 19 nucleotides Rv0099: Rv0099 - Possible fatty-acid-CoA ligase FadD10 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase), at 108,156 to 109,778 Rv0099 Rv0100: Rv0100 - Conserved hypothetical protein, at 109,783 to 110,019 Rv0100 Rv0101: Rv0101 - Probable peptide synthetase Nrp (peptide synthase), at 110,001 to 117,539 Rv0101 Position (kb) 109 110 111Strain fitness (log2 ratio) -2 -1 0 1at 108.807 kb on + strand, within Rv0099at 108.854 kb on + strand, within Rv0099at 108.854 kb on + strand, within Rv0099at 108.855 kb on - strand, within Rv0099at 109.286 kb on - strand, within Rv0099at 109.286 kb on - strand, within Rv0099at 109.822 kb on + strand, within Rv0100at 109.916 kb on + strand, within Rv0100at 109.917 kb on - strand, within Rv0100at 110.041 kb on - strandat 110.075 kb on - strandat 110.075 kb on - strandat 110.149 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction no stress control
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108,807 + Rv0099 0.40 +0.8
108,854 + Rv0099 0.43 -0.2
108,854 + Rv0099 0.43 -0.0
108,855 - Rv0099 0.43 -2.1
109,286 - Rv0099 0.70 +0.3
109,286 - Rv0099 0.70 +0.0
109,822 + Rv0100 0.16 -1.0
109,916 + Rv0100 0.56 -1.0
109,917 - Rv0100 0.57 +1.5
110,041 - -0.4
110,075 - -0.9
110,075 - -0.3
110,149 + +0.8

Or see this region's nucleotide sequence