Experiment: TSB plate, 1:1 coculture with Microbacterium trichothecenolyticum CFG_272
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00515 and MPMX20_00516 are separated by 93 nucleotides MPMX20_00516 and MPMX20_00517 are separated by 95 nucleotides MPMX20_00517 and MPMX20_00518 are separated by 41 nucleotides MPMX20_00518 and MPMX20_00519 are separated by 336 nucleotides
MPMX20_00515: MPMX20_00515 - UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl- meso-2,6-diaminoheptandioate ligase, at 532,985 to 534,361
_00515
MPMX20_00516: MPMX20_00516 - hypothetical protein, at 534,455 to 535,006
_00516
MPMX20_00517: MPMX20_00517 - Metalloprotease PmbA, at 535,102 to 536,454
_00517
MPMX20_00518: MPMX20_00518 - Soluble cytochrome b562, at 536,496 to 536,882
_00518
MPMX20_00519: MPMX20_00519 - hypothetical protein, at 537,219 to 537,539
_00519
Position (kb) 535
536
537 Strain fitness (log2 ratio) -2
-1
0
1
2 at 534.114 kb on + strand, within MPMX20_00515 at 534.122 kb on - strand, within MPMX20_00515 at 534.205 kb on + strand, within MPMX20_00515 at 534.211 kb on - strand, within MPMX20_00515 at 534.244 kb on + strand at 534.259 kb on + strand at 534.335 kb on - strand at 534.411 kb on - strand at 534.427 kb on + strand at 534.435 kb on - strand at 534.437 kb on - strand at 534.448 kb on - strand at 534.460 kb on - strand at 535.036 kb on - strand at 535.047 kb on + strand at 535.063 kb on - strand at 535.070 kb on + strand at 535.070 kb on + strand at 535.078 kb on - strand at 535.090 kb on + strand at 535.100 kb on + strand at 535.100 kb on - strand at 535.100 kb on - strand at 535.100 kb on - strand at 535.108 kb on - strand at 535.125 kb on - strand at 535.143 kb on + strand at 535.151 kb on - strand at 535.177 kb on - strand at 535.370 kb on + strand, within MPMX20_00517 at 535.457 kb on - strand, within MPMX20_00517 at 535.542 kb on - strand, within MPMX20_00517 at 535.542 kb on - strand, within MPMX20_00517 at 535.625 kb on + strand, within MPMX20_00517 at 535.644 kb on + strand, within MPMX20_00517 at 535.649 kb on - strand, within MPMX20_00517 at 535.674 kb on - strand, within MPMX20_00517 at 535.753 kb on - strand, within MPMX20_00517 at 535.970 kb on + strand, within MPMX20_00517 at 535.970 kb on + strand, within MPMX20_00517 at 535.978 kb on - strand, within MPMX20_00517 at 535.978 kb on - strand, within MPMX20_00517 at 536.014 kb on - strand, within MPMX20_00517 at 536.141 kb on + strand, within MPMX20_00517 at 536.164 kb on + strand, within MPMX20_00517 at 536.170 kb on + strand, within MPMX20_00517 at 536.178 kb on - strand, within MPMX20_00517 at 536.185 kb on - strand, within MPMX20_00517 at 536.185 kb on - strand, within MPMX20_00517 at 536.185 kb on - strand, within MPMX20_00517 at 536.185 kb on - strand, within MPMX20_00517 at 536.185 kb on - strand, within MPMX20_00517 at 536.286 kb on - strand, within MPMX20_00517 at 536.288 kb on + strand, within MPMX20_00517 at 536.288 kb on + strand, within MPMX20_00517 at 536.290 kb on + strand, within MPMX20_00517 at 536.296 kb on - strand, within MPMX20_00517 at 536.343 kb on + strand at 536.343 kb on + strand at 536.343 kb on + strand at 536.352 kb on - strand at 536.384 kb on - strand at 536.399 kb on + strand at 536.399 kb on + strand at 536.399 kb on + strand at 536.399 kb on + strand at 536.399 kb on + strand at 536.399 kb on + strand at 536.399 kb on + strand at 536.399 kb on + strand at 536.399 kb on + strand at 536.399 kb on + strand at 536.399 kb on + strand at 536.399 kb on + strand at 536.399 kb on + strand at 536.404 kb on + strand at 536.407 kb on - strand at 536.407 kb on - strand at 536.407 kb on - strand at 536.407 kb on - strand at 536.407 kb on - strand at 536.407 kb on - strand at 536.407 kb on - strand at 536.407 kb on - strand at 536.407 kb on - strand at 536.407 kb on - strand at 536.407 kb on - strand at 536.409 kb on + strand at 536.409 kb on + strand at 536.409 kb on + strand at 536.417 kb on - strand at 536.417 kb on - strand at 536.459 kb on - strand at 536.466 kb on - strand at 536.508 kb on - strand at 536.527 kb on + strand at 536.545 kb on - strand, within MPMX20_00518 at 536.545 kb on - strand, within MPMX20_00518 at 536.576 kb on + strand, within MPMX20_00518 at 536.600 kb on + strand, within MPMX20_00518 at 536.608 kb on - strand, within MPMX20_00518 at 536.608 kb on - strand, within MPMX20_00518 at 536.660 kb on + strand, within MPMX20_00518 at 536.733 kb on - strand, within MPMX20_00518 at 536.752 kb on + strand, within MPMX20_00518 at 536.760 kb on + strand, within MPMX20_00518 at 536.760 kb on - strand, within MPMX20_00518 at 536.774 kb on + strand, within MPMX20_00518 at 536.774 kb on + strand, within MPMX20_00518 at 536.782 kb on - strand, within MPMX20_00518 at 536.782 kb on - strand, within MPMX20_00518 at 536.782 kb on - strand, within MPMX20_00518 at 536.784 kb on + strand, within MPMX20_00518 at 536.784 kb on + strand, within MPMX20_00518 at 536.792 kb on - strand, within MPMX20_00518 at 536.820 kb on + strand, within MPMX20_00518 at 536.840 kb on + strand, within MPMX20_00518 at 536.840 kb on + strand, within MPMX20_00518 at 536.848 kb on - strand at 537.012 kb on + strand at 537.020 kb on - strand at 537.088 kb on + strand at 537.090 kb on + strand at 537.093 kb on + strand at 537.093 kb on + strand at 537.101 kb on - strand at 537.101 kb on - strand at 537.101 kb on - strand at 537.101 kb on - strand at 537.101 kb on - strand at 537.101 kb on - strand at 537.101 kb on - strand at 537.104 kb on + strand at 537.104 kb on + strand at 537.104 kb on + strand at 537.119 kb on - strand at 537.196 kb on + strand at 537.216 kb on + strand at 537.224 kb on - strand at 537.224 kb on - strand at 537.242 kb on + strand at 537.250 kb on + strand at 537.260 kb on + strand, within MPMX20_00519 at 537.268 kb on - strand, within MPMX20_00519 at 537.274 kb on - strand, within MPMX20_00519 at 537.281 kb on + strand, within MPMX20_00519 at 537.281 kb on + strand, within MPMX20_00519 at 537.281 kb on + strand, within MPMX20_00519 at 537.281 kb on + strand, within MPMX20_00519 at 537.281 kb on + strand, within MPMX20_00519 at 537.281 kb on + strand, within MPMX20_00519 at 537.281 kb on + strand, within MPMX20_00519 at 537.289 kb on - strand, within MPMX20_00519 at 537.291 kb on - strand, within MPMX20_00519 at 537.310 kb on - strand, within MPMX20_00519 at 537.324 kb on + strand, within MPMX20_00519 at 537.351 kb on - strand, within MPMX20_00519 at 537.379 kb on + strand, within MPMX20_00519 at 537.393 kb on + strand, within MPMX20_00519 at 537.398 kb on + strand, within MPMX20_00519 at 537.406 kb on - strand, within MPMX20_00519 at 537.406 kb on - strand, within MPMX20_00519
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, 1:1 coculture with Microbacterium trichothecenolyticum CFG_272 remove 534,114 + MPMX20_00515 0.82 -1.5 534,122 - MPMX20_00515 0.83 +1.1 534,205 + MPMX20_00515 0.89 -0.9 534,211 - MPMX20_00515 0.89 -0.6 534,244 + -0.2 534,259 + +0.3 534,335 - +0.4 534,411 - -2.1 534,427 + +0.1 534,435 - +1.4 534,437 - -1.1 534,448 - -1.1 534,460 - +2.1 535,036 - -0.5 535,047 + +1.4 535,063 - -1.8 535,070 + +0.0 535,070 + -0.5 535,078 - -0.9 535,090 + -0.6 535,100 + +0.4 535,100 - -0.9 535,100 - +0.3 535,100 - -2.3 535,108 - +1.4 535,125 - -1.3 535,143 + -1.2 535,151 - -0.3 535,177 - -0.8 535,370 + MPMX20_00517 0.20 -0.1 535,457 - MPMX20_00517 0.26 -1.2 535,542 - MPMX20_00517 0.33 +0.4 535,542 - MPMX20_00517 0.33 +1.0 535,625 + MPMX20_00517 0.39 -1.2 535,644 + MPMX20_00517 0.40 +0.0 535,649 - MPMX20_00517 0.40 -1.5 535,674 - MPMX20_00517 0.42 -2.5 535,753 - MPMX20_00517 0.48 -0.6 535,970 + MPMX20_00517 0.64 -0.2 535,970 + MPMX20_00517 0.64 +1.4 535,978 - MPMX20_00517 0.65 -1.8 535,978 - MPMX20_00517 0.65 -0.6 536,014 - MPMX20_00517 0.67 -1.3 536,141 + MPMX20_00517 0.77 +0.4 536,164 + MPMX20_00517 0.78 -1.3 536,170 + MPMX20_00517 0.79 -2.2 536,178 - MPMX20_00517 0.80 +0.6 536,185 - MPMX20_00517 0.80 +0.4 536,185 - MPMX20_00517 0.80 +0.2 536,185 - MPMX20_00517 0.80 -0.8 536,185 - MPMX20_00517 0.80 +0.6 536,185 - MPMX20_00517 0.80 +0.0 536,286 - MPMX20_00517 0.88 +0.0 536,288 + MPMX20_00517 0.88 -1.2 536,288 + MPMX20_00517 0.88 -1.5 536,290 + MPMX20_00517 0.88 -0.3 536,296 - MPMX20_00517 0.88 -1.5 536,343 + +1.3 536,343 + +0.3 536,343 + +1.3 536,352 - +0.7 536,384 - +0.6 536,399 + +0.3 536,399 + -2.2 536,399 + -0.4 536,399 + -1.0 536,399 + -0.7 536,399 + +1.5 536,399 + +1.1 536,399 + -2.1 536,399 + +1.6 536,399 + +0.8 536,399 + -1.0 536,399 + +2.3 536,399 + +1.3 536,404 + +0.8 536,407 - +1.3 536,407 - +2.3 536,407 - -1.2 536,407 - +0.5 536,407 - -1.4 536,407 - -0.8 536,407 - -0.7 536,407 - -1.8 536,407 - -2.2 536,407 - +1.0 536,407 - +0.0 536,409 + +0.1 536,409 + +2.3 536,409 + +0.3 536,417 - -0.1 536,417 - -1.4 536,459 - -0.2 536,466 - +2.3 536,508 - -0.3 536,527 + +0.5 536,545 - MPMX20_00518 0.13 -1.0 536,545 - MPMX20_00518 0.13 +0.5 536,576 + MPMX20_00518 0.21 -0.4 536,600 + MPMX20_00518 0.27 +2.5 536,608 - MPMX20_00518 0.29 +1.7 536,608 - MPMX20_00518 0.29 -1.5 536,660 + MPMX20_00518 0.42 -0.7 536,733 - MPMX20_00518 0.61 -1.8 536,752 + MPMX20_00518 0.66 +0.1 536,760 + MPMX20_00518 0.68 -0.9 536,760 - MPMX20_00518 0.68 +0.8 536,774 + MPMX20_00518 0.72 +0.3 536,774 + MPMX20_00518 0.72 -0.4 536,782 - MPMX20_00518 0.74 +0.3 536,782 - MPMX20_00518 0.74 -1.1 536,782 - MPMX20_00518 0.74 -0.2 536,784 + MPMX20_00518 0.74 +0.4 536,784 + MPMX20_00518 0.74 +1.5 536,792 - MPMX20_00518 0.76 -1.0 536,820 + MPMX20_00518 0.84 +1.0 536,840 + MPMX20_00518 0.89 -1.9 536,840 + MPMX20_00518 0.89 +0.3 536,848 - -0.2 537,012 + -0.8 537,020 - +2.3 537,088 + -0.5 537,090 + +0.5 537,093 + -0.6 537,093 + -0.8 537,101 - +1.3 537,101 - +0.6 537,101 - +2.3 537,101 - -0.1 537,101 - +0.1 537,101 - +0.9 537,101 - -0.5 537,104 + -0.8 537,104 + +0.0 537,104 + +0.8 537,119 - +0.5 537,196 + -0.8 537,216 + +1.0 537,224 - -2.0 537,224 - +0.9 537,242 + -1.9 537,250 + -1.3 537,260 + MPMX20_00519 0.13 +0.3 537,268 - MPMX20_00519 0.15 +0.0 537,274 - MPMX20_00519 0.17 -0.0 537,281 + MPMX20_00519 0.19 +0.1 537,281 + MPMX20_00519 0.19 -0.9 537,281 + MPMX20_00519 0.19 -0.6 537,281 + MPMX20_00519 0.19 -1.3 537,281 + MPMX20_00519 0.19 -1.5 537,281 + MPMX20_00519 0.19 -0.7 537,281 + MPMX20_00519 0.19 -0.3 537,289 - MPMX20_00519 0.22 +0.0 537,291 - MPMX20_00519 0.22 +0.3 537,310 - MPMX20_00519 0.28 -1.1 537,324 + MPMX20_00519 0.33 +0.3 537,351 - MPMX20_00519 0.41 -0.2 537,379 + MPMX20_00519 0.50 +0.5 537,393 + MPMX20_00519 0.54 -1.2 537,398 + MPMX20_00519 0.56 -0.1 537,406 - MPMX20_00519 0.58 -1.1 537,406 - MPMX20_00519 0.58 -0.0
Or see this region's nucleotide sequence