Experiment: Hydroxide 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_84 and Pf6N2E2_85 are separated by 128 nucleotides Pf6N2E2_85 and Pf6N2E2_86 are separated by 2 nucleotides
Pf6N2E2_84 - hypothetical protein, at 129,585 to 129,857
_84
Pf6N2E2_85 - FIG045511: hypothetical antitoxin (to FIG022160: hypothetical toxin), at 129,986 to 130,294
_85
Pf6N2E2_86 - FIG022160: hypothetical toxin, at 130,297 to 130,467
_86
Position (kb)
129
130 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 128.657 kb on + strand at 128.658 kb on - strand at 128.658 kb on - strand at 128.672 kb on + strand at 128.673 kb on - strand at 128.673 kb on - strand at 128.679 kb on + strand at 128.680 kb on - strand at 128.696 kb on + strand at 128.697 kb on - strand at 128.697 kb on - strand at 128.698 kb on + strand at 128.698 kb on + strand at 128.699 kb on - strand at 128.699 kb on - strand at 128.712 kb on + strand at 128.764 kb on - strand at 128.764 kb on - strand at 128.764 kb on - strand at 128.765 kb on + strand at 128.765 kb on + strand at 128.765 kb on + strand at 128.765 kb on + strand at 128.765 kb on + strand at 128.766 kb on - strand at 128.766 kb on - strand at 128.766 kb on - strand at 128.847 kb on + strand at 128.847 kb on + strand at 128.848 kb on - strand at 128.848 kb on - strand at 128.945 kb on + strand at 129.015 kb on - strand at 129.334 kb on + strand at 129.358 kb on + strand at 129.358 kb on + strand at 129.358 kb on + strand at 129.359 kb on - strand at 129.359 kb on - strand at 129.363 kb on - strand at 129.368 kb on + strand at 129.389 kb on + strand at 129.389 kb on + strand at 129.389 kb on + strand at 129.389 kb on + strand at 129.389 kb on + strand at 129.390 kb on - strand at 129.483 kb on + strand at 129.483 kb on + strand at 129.484 kb on - strand at 129.484 kb on - strand at 129.484 kb on - strand at 129.558 kb on + strand at 129.558 kb on + strand at 129.558 kb on + strand at 129.577 kb on + strand at 129.577 kb on + strand at 129.577 kb on + strand at 129.578 kb on - strand at 129.793 kb on + strand, within Pf6N2E2_84 at 129.794 kb on - strand, within Pf6N2E2_84 at 129.855 kb on + strand at 129.855 kb on + strand at 129.855 kb on + strand at 129.873 kb on + strand at 129.873 kb on + strand at 129.873 kb on + strand at 129.873 kb on + strand at 129.873 kb on + strand at 129.874 kb on - strand at 129.874 kb on - strand at 129.874 kb on - strand at 129.874 kb on - strand at 129.874 kb on - strand at 129.874 kb on - strand at 129.874 kb on - strand at 129.874 kb on - strand at 129.874 kb on - strand at 129.875 kb on + strand at 129.875 kb on + strand at 129.875 kb on + strand at 129.875 kb on + strand at 129.875 kb on + strand at 129.892 kb on + strand at 129.892 kb on + strand at 129.893 kb on - strand at 129.915 kb on - strand at 129.915 kb on - strand at 129.921 kb on - strand at 129.921 kb on - strand at 129.976 kb on - strand at 130.003 kb on + strand at 130.004 kb on - strand at 130.004 kb on - strand at 130.061 kb on + strand, within Pf6N2E2_85 at 130.062 kb on - strand, within Pf6N2E2_85 at 130.104 kb on + strand, within Pf6N2E2_85 at 130.105 kb on - strand, within Pf6N2E2_85 at 130.105 kb on - strand, within Pf6N2E2_85 at 130.105 kb on - strand, within Pf6N2E2_85 at 130.105 kb on - strand, within Pf6N2E2_85 at 130.171 kb on - strand, within Pf6N2E2_85 at 130.227 kb on + strand, within Pf6N2E2_85 at 130.228 kb on - strand, within Pf6N2E2_85 at 130.228 kb on - strand, within Pf6N2E2_85 at 130.228 kb on - strand, within Pf6N2E2_85 at 130.228 kb on - strand, within Pf6N2E2_85 at 130.228 kb on - strand, within Pf6N2E2_85 at 130.256 kb on + strand, within Pf6N2E2_85 at 130.403 kb on + strand, within Pf6N2E2_86 at 130.403 kb on + strand, within Pf6N2E2_86 at 130.404 kb on - strand, within Pf6N2E2_86 at 130.423 kb on + strand, within Pf6N2E2_86 at 130.423 kb on + strand, within Pf6N2E2_86 at 130.424 kb on - strand, within Pf6N2E2_86 at 130.424 kb on - strand, within Pf6N2E2_86 at 130.446 kb on + strand, within Pf6N2E2_86 at 130.446 kb on + strand, within Pf6N2E2_86 at 130.447 kb on - strand, within Pf6N2E2_86 at 130.587 kb on + strand at 130.587 kb on + strand at 130.587 kb on + strand at 130.588 kb on - strand at 130.613 kb on - strand at 130.743 kb on + strand at 130.743 kb on + strand at 130.744 kb on - strand at 130.754 kb on + strand at 130.754 kb on + strand at 130.754 kb on + strand at 130.755 kb on - strand at 130.755 kb on - strand at 130.755 kb on - strand at 130.764 kb on - strand at 130.812 kb on + strand at 130.812 kb on + strand at 130.812 kb on + strand at 130.812 kb on + strand at 130.813 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxide 0.05 M remove 128,657 + +0.7 128,658 - -0.9 128,658 - +0.4 128,672 + -0.5 128,673 - +0.0 128,673 - +0.6 128,679 + +0.0 128,680 - -0.4 128,696 + +0.1 128,697 - -0.3 128,697 - -0.1 128,698 + -0.1 128,698 + -1.0 128,699 - +1.2 128,699 - +0.1 128,712 + -0.4 128,764 - +0.1 128,764 - -1.0 128,764 - -1.6 128,765 + +0.5 128,765 + -2.0 128,765 + -1.3 128,765 + +0.2 128,765 + -2.8 128,766 - -2.4 128,766 - +0.8 128,766 - -0.2 128,847 + -0.2 128,847 + -2.0 128,848 - -0.2 128,848 - -1.0 128,945 + +0.3 129,015 - +0.1 129,334 + +2.4 129,358 + -1.1 129,358 + +1.6 129,358 + -0.1 129,359 - -0.7 129,359 - +0.0 129,363 - +0.0 129,368 + +0.5 129,389 + +0.9 129,389 + +0.2 129,389 + -0.8 129,389 + +0.1 129,389 + +0.3 129,390 - -0.2 129,483 + +0.2 129,483 + -0.9 129,484 - +0.7 129,484 - +0.1 129,484 - -1.0 129,558 + +0.1 129,558 + -1.2 129,558 + -0.1 129,577 + +0.5 129,577 + +0.3 129,577 + -0.4 129,578 - +0.0 129,793 + Pf6N2E2_84 0.76 -0.4 129,794 - Pf6N2E2_84 0.77 -1.3 129,855 + +0.1 129,855 + +0.1 129,855 + +0.3 129,873 + +1.0 129,873 + +1.4 129,873 + +0.4 129,873 + +0.4 129,873 + +0.7 129,874 - -0.4 129,874 - -0.3 129,874 - -0.0 129,874 - -0.9 129,874 - +0.3 129,874 - +0.2 129,874 - -1.1 129,874 - -0.4 129,874 - -0.4 129,875 + -0.2 129,875 + +0.0 129,875 + +0.5 129,875 + -0.5 129,875 + -0.1 129,892 + +0.0 129,892 + +0.6 129,893 - +0.5 129,915 - -0.7 129,915 - +0.4 129,921 - +0.0 129,921 - +0.1 129,976 - -0.5 130,003 + +1.8 130,004 - +0.9 130,004 - -0.1 130,061 + Pf6N2E2_85 0.24 +0.5 130,062 - Pf6N2E2_85 0.25 -1.2 130,104 + Pf6N2E2_85 0.38 -0.3 130,105 - Pf6N2E2_85 0.39 +0.5 130,105 - Pf6N2E2_85 0.39 -0.5 130,105 - Pf6N2E2_85 0.39 -0.7 130,105 - Pf6N2E2_85 0.39 -2.5 130,171 - Pf6N2E2_85 0.60 -0.4 130,227 + Pf6N2E2_85 0.78 -0.4 130,228 - Pf6N2E2_85 0.78 +0.3 130,228 - Pf6N2E2_85 0.78 +0.1 130,228 - Pf6N2E2_85 0.78 -0.5 130,228 - Pf6N2E2_85 0.78 +0.4 130,228 - Pf6N2E2_85 0.78 -0.4 130,256 + Pf6N2E2_85 0.87 +1.3 130,403 + Pf6N2E2_86 0.62 +0.8 130,403 + Pf6N2E2_86 0.62 +0.1 130,404 - Pf6N2E2_86 0.63 -0.7 130,423 + Pf6N2E2_86 0.74 +0.0 130,423 + Pf6N2E2_86 0.74 -0.1 130,424 - Pf6N2E2_86 0.74 +0.3 130,424 - Pf6N2E2_86 0.74 +0.0 130,446 + Pf6N2E2_86 0.87 -0.6 130,446 + Pf6N2E2_86 0.87 -0.3 130,447 - Pf6N2E2_86 0.88 -1.4 130,587 + -0.3 130,587 + +1.1 130,587 + +1.7 130,588 - +0.0 130,613 - -0.5 130,743 + +0.0 130,743 + -0.4 130,744 - -1.5 130,754 + +0.5 130,754 + -0.3 130,754 + -1.8 130,755 - +0.4 130,755 - +0.6 130,755 - -1.9 130,764 - +1.4 130,812 + +0.1 130,812 + +0.2 130,812 + -0.3 130,812 + -0.6 130,813 - -0.3
Or see this region's nucleotide sequence