Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4495

Experiment: dimethyl sulfone 0.25 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4494 and Pf6N2E2_4495 are separated by 24 nucleotidesPf6N2E2_4495 and Pf6N2E2_4496 are separated by 14 nucleotides Pf6N2E2_4494 - Adenylate cyclase (EC 4.6.1.1), at 5,226,306 to 5,227,673 _4494 Pf6N2E2_4495 - hypothetical protein, at 5,227,698 to 5,227,817 _4495 Pf6N2E2_4496 - Acetylornithine deacetylase (EC 3.5.1.16), at 5,227,832 to 5,228,989 _4496 Position (kb) 5227 5228Strain fitness (log2 ratio) -2 -1 0 1 2at 5227.049 kb on - strand, within Pf6N2E2_4494at 5227.049 kb on - strand, within Pf6N2E2_4494at 5227.064 kb on - strand, within Pf6N2E2_4494at 5227.417 kb on + strand, within Pf6N2E2_4494at 5227.417 kb on + strand, within Pf6N2E2_4494at 5227.418 kb on - strand, within Pf6N2E2_4494at 5227.549 kb on + strandat 5227.549 kb on + strandat 5227.550 kb on - strandat 5227.550 kb on - strandat 5227.739 kb on + strand, within Pf6N2E2_4495at 5227.739 kb on + strand, within Pf6N2E2_4495at 5227.739 kb on + strand, within Pf6N2E2_4495at 5227.739 kb on + strand, within Pf6N2E2_4495at 5227.739 kb on + strand, within Pf6N2E2_4495at 5227.758 kb on - strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strandat 5227.797 kb on - strand, within Pf6N2E2_4495at 5227.797 kb on - strand, within Pf6N2E2_4495at 5227.797 kb on - strand, within Pf6N2E2_4495at 5227.797 kb on - strand, within Pf6N2E2_4495at 5227.797 kb on - strand, within Pf6N2E2_4495at 5227.797 kb on - strand, within Pf6N2E2_4495at 5227.797 kb on - strand, within Pf6N2E2_4495at 5227.815 kb on + strandat 5227.815 kb on + strandat 5227.815 kb on + strandat 5227.816 kb on - strandat 5227.816 kb on - strandat 5228.046 kb on + strand, within Pf6N2E2_4496at 5228.686 kb on + strand, within Pf6N2E2_4496at 5228.686 kb on + strand, within Pf6N2E2_4496at 5228.702 kb on + strand, within Pf6N2E2_4496at 5228.702 kb on + strand, within Pf6N2E2_4496

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Per-strain Table

Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M
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5,227,049 - Pf6N2E2_4494 0.54 -2.3
5,227,049 - Pf6N2E2_4494 0.54 +0.4
5,227,064 - Pf6N2E2_4494 0.55 -0.5
5,227,417 + Pf6N2E2_4494 0.81 +0.8
5,227,417 + Pf6N2E2_4494 0.81 +1.0
5,227,418 - Pf6N2E2_4494 0.81 -2.2
5,227,549 + -1.5
5,227,549 + -1.8
5,227,550 - -0.1
5,227,550 - -0.4
5,227,739 + Pf6N2E2_4495 0.34 +0.7
5,227,739 + Pf6N2E2_4495 0.34 -0.0
5,227,739 + Pf6N2E2_4495 0.34 -0.9
5,227,739 + Pf6N2E2_4495 0.34 +1.2
5,227,739 + Pf6N2E2_4495 0.34 -2.0
5,227,758 - Pf6N2E2_4495 0.50 +0.6
5,227,796 + Pf6N2E2_4495 0.82 +1.4
5,227,796 + Pf6N2E2_4495 0.82 +0.6
5,227,796 + Pf6N2E2_4495 0.82 -0.6
5,227,796 + Pf6N2E2_4495 0.82 +2.3
5,227,796 + Pf6N2E2_4495 0.82 -0.1
5,227,796 + Pf6N2E2_4495 0.82 +0.9
5,227,796 + Pf6N2E2_4495 0.82 -0.6
5,227,796 + Pf6N2E2_4495 0.82 +0.6
5,227,796 + Pf6N2E2_4495 0.82 -0.5
5,227,796 + +1.7
5,227,797 - Pf6N2E2_4495 0.82 -1.5
5,227,797 - Pf6N2E2_4495 0.82 +1.4
5,227,797 - Pf6N2E2_4495 0.82 +0.9
5,227,797 - Pf6N2E2_4495 0.82 +0.4
5,227,797 - Pf6N2E2_4495 0.82 +0.3
5,227,797 - Pf6N2E2_4495 0.82 +2.4
5,227,797 - Pf6N2E2_4495 0.82 +1.4
5,227,815 + +1.3
5,227,815 + +1.1
5,227,815 + -0.7
5,227,816 - -0.9
5,227,816 - -0.2
5,228,046 + Pf6N2E2_4496 0.18 +0.7
5,228,686 + Pf6N2E2_4496 0.74 +0.7
5,228,686 + Pf6N2E2_4496 0.74 +1.1
5,228,702 + Pf6N2E2_4496 0.75 -1.0
5,228,702 + Pf6N2E2_4496 0.75 -0.1

Or see this region's nucleotide sequence