Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4494 and Pf6N2E2_4495 are separated by 24 nucleotides Pf6N2E2_4495 and Pf6N2E2_4496 are separated by 14 nucleotides
Pf6N2E2_4494 - Adenylate cyclase (EC 4.6.1.1), at 5,226,306 to 5,227,673
_4494
Pf6N2E2_4495 - hypothetical protein, at 5,227,698 to 5,227,817
_4495
Pf6N2E2_4496 - Acetylornithine deacetylase (EC 3.5.1.16), at 5,227,832 to 5,228,989
_4496
Position (kb)
5227
5228 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5227.049 kb on - strand, within Pf6N2E2_4494 at 5227.049 kb on - strand, within Pf6N2E2_4494 at 5227.064 kb on - strand, within Pf6N2E2_4494 at 5227.417 kb on + strand, within Pf6N2E2_4494 at 5227.417 kb on + strand, within Pf6N2E2_4494 at 5227.418 kb on - strand, within Pf6N2E2_4494 at 5227.549 kb on + strand at 5227.549 kb on + strand at 5227.550 kb on - strand at 5227.550 kb on - strand at 5227.739 kb on + strand, within Pf6N2E2_4495 at 5227.739 kb on + strand, within Pf6N2E2_4495 at 5227.739 kb on + strand, within Pf6N2E2_4495 at 5227.739 kb on + strand, within Pf6N2E2_4495 at 5227.739 kb on + strand, within Pf6N2E2_4495 at 5227.758 kb on - strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.815 kb on + strand at 5227.815 kb on + strand at 5227.815 kb on + strand at 5227.816 kb on - strand at 5227.816 kb on - strand at 5228.046 kb on + strand, within Pf6N2E2_4496 at 5228.686 kb on + strand, within Pf6N2E2_4496 at 5228.686 kb on + strand, within Pf6N2E2_4496 at 5228.702 kb on + strand, within Pf6N2E2_4496 at 5228.702 kb on + strand, within Pf6N2E2_4496
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 5,227,049 - Pf6N2E2_4494 0.54 -2.3 5,227,049 - Pf6N2E2_4494 0.54 +0.4 5,227,064 - Pf6N2E2_4494 0.55 -0.5 5,227,417 + Pf6N2E2_4494 0.81 +0.8 5,227,417 + Pf6N2E2_4494 0.81 +1.0 5,227,418 - Pf6N2E2_4494 0.81 -2.2 5,227,549 + -1.5 5,227,549 + -1.8 5,227,550 - -0.1 5,227,550 - -0.4 5,227,739 + Pf6N2E2_4495 0.34 +0.7 5,227,739 + Pf6N2E2_4495 0.34 -0.0 5,227,739 + Pf6N2E2_4495 0.34 -0.9 5,227,739 + Pf6N2E2_4495 0.34 +1.2 5,227,739 + Pf6N2E2_4495 0.34 -2.0 5,227,758 - Pf6N2E2_4495 0.50 +0.6 5,227,796 + Pf6N2E2_4495 0.82 +1.4 5,227,796 + Pf6N2E2_4495 0.82 +0.6 5,227,796 + Pf6N2E2_4495 0.82 -0.6 5,227,796 + Pf6N2E2_4495 0.82 +2.3 5,227,796 + Pf6N2E2_4495 0.82 -0.1 5,227,796 + Pf6N2E2_4495 0.82 +0.9 5,227,796 + Pf6N2E2_4495 0.82 -0.6 5,227,796 + Pf6N2E2_4495 0.82 +0.6 5,227,796 + Pf6N2E2_4495 0.82 -0.5 5,227,796 + +1.7 5,227,797 - Pf6N2E2_4495 0.82 -1.5 5,227,797 - Pf6N2E2_4495 0.82 +1.4 5,227,797 - Pf6N2E2_4495 0.82 +0.9 5,227,797 - Pf6N2E2_4495 0.82 +0.4 5,227,797 - Pf6N2E2_4495 0.82 +0.3 5,227,797 - Pf6N2E2_4495 0.82 +2.4 5,227,797 - Pf6N2E2_4495 0.82 +1.4 5,227,815 + +1.3 5,227,815 + +1.1 5,227,815 + -0.7 5,227,816 - -0.9 5,227,816 - -0.2 5,228,046 + Pf6N2E2_4496 0.18 +0.7 5,228,686 + Pf6N2E2_4496 0.74 +0.7 5,228,686 + Pf6N2E2_4496 0.74 +1.1 5,228,702 + Pf6N2E2_4496 0.75 -1.0 5,228,702 + Pf6N2E2_4496 0.75 -0.1
Or see this region's nucleotide sequence