Experiment: nitrate 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1097 and Pf6N2E2_1098 are separated by 144 nucleotides Pf6N2E2_1098 and Pf6N2E2_1099 are separated by 69 nucleotides Pf6N2E2_1099 and Pf6N2E2_1100 are separated by 178 nucleotides
Pf6N2E2_1097 - Translation initiation inhibitor, at 1,284,678 to 1,285,028
_1097
Pf6N2E2_1098 - Arginine/ornithine ABC transporter, periplasmic arginine/ornithine binding protein, at 1,285,173 to 1,285,955
_1098
Pf6N2E2_1099 - Hypothetical protein GlcG in glycolate utilization operon, at 1,286,025 to 1,286,450
_1099
Pf6N2E2_1100 - L-rhamnose operon transcriptional activator RhaR, at 1,286,629 to 1,287,465
_1100
Position (kb)
1286
1287 Strain fitness (log2 ratio)
-1
0
1
2 at 1285.149 kb on + strand at 1285.182 kb on - strand at 1285.333 kb on - strand, within Pf6N2E2_1098 at 1285.614 kb on + strand, within Pf6N2E2_1098 at 1285.651 kb on + strand, within Pf6N2E2_1098 at 1285.651 kb on + strand, within Pf6N2E2_1098 at 1285.651 kb on + strand, within Pf6N2E2_1098 at 1285.660 kb on - strand, within Pf6N2E2_1098 at 1285.679 kb on - strand, within Pf6N2E2_1098 at 1285.679 kb on - strand, within Pf6N2E2_1098 at 1285.679 kb on - strand, within Pf6N2E2_1098 at 1285.809 kb on + strand, within Pf6N2E2_1098 at 1285.810 kb on - strand, within Pf6N2E2_1098 at 1285.905 kb on + strand at 1285.905 kb on + strand at 1285.926 kb on + strand at 1285.926 kb on + strand at 1285.926 kb on + strand at 1285.927 kb on - strand at 1285.944 kb on + strand at 1285.944 kb on + strand at 1285.985 kb on + strand at 1286.005 kb on + strand at 1286.006 kb on - strand at 1286.158 kb on + strand, within Pf6N2E2_1099 at 1286.193 kb on - strand, within Pf6N2E2_1099 at 1286.237 kb on + strand, within Pf6N2E2_1099 at 1286.238 kb on - strand, within Pf6N2E2_1099 at 1286.460 kb on - strand at 1286.460 kb on - strand at 1286.498 kb on + strand at 1286.498 kb on + strand at 1286.510 kb on + strand at 1286.511 kb on - strand at 1286.511 kb on - strand at 1286.713 kb on + strand, within Pf6N2E2_1100 at 1286.714 kb on - strand, within Pf6N2E2_1100 at 1286.737 kb on + strand, within Pf6N2E2_1100 at 1286.737 kb on + strand, within Pf6N2E2_1100 at 1286.737 kb on + strand, within Pf6N2E2_1100 at 1286.738 kb on - strand, within Pf6N2E2_1100 at 1286.738 kb on - strand, within Pf6N2E2_1100 at 1286.738 kb on - strand, within Pf6N2E2_1100 at 1286.802 kb on + strand, within Pf6N2E2_1100 at 1286.802 kb on + strand, within Pf6N2E2_1100 at 1286.878 kb on + strand, within Pf6N2E2_1100 at 1286.878 kb on + strand, within Pf6N2E2_1100 at 1286.890 kb on + strand, within Pf6N2E2_1100 at 1287.073 kb on - strand, within Pf6N2E2_1100 at 1287.286 kb on + strand, within Pf6N2E2_1100 at 1287.287 kb on - strand, within Pf6N2E2_1100 at 1287.287 kb on - strand, within Pf6N2E2_1100 at 1287.287 kb on - strand, within Pf6N2E2_1100 at 1287.287 kb on - strand, within Pf6N2E2_1100 at 1287.287 kb on - strand, within Pf6N2E2_1100 at 1287.287 kb on - strand, within Pf6N2E2_1100 at 1287.434 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 0.125 M remove 1,285,149 + +0.1 1,285,182 - +0.5 1,285,333 - Pf6N2E2_1098 0.20 +1.0 1,285,614 + Pf6N2E2_1098 0.56 +2.1 1,285,651 + Pf6N2E2_1098 0.61 -1.1 1,285,651 + Pf6N2E2_1098 0.61 +1.1 1,285,651 + Pf6N2E2_1098 0.61 -1.1 1,285,660 - Pf6N2E2_1098 0.62 -0.1 1,285,679 - Pf6N2E2_1098 0.65 +1.0 1,285,679 - Pf6N2E2_1098 0.65 +0.0 1,285,679 - Pf6N2E2_1098 0.65 +1.0 1,285,809 + Pf6N2E2_1098 0.81 -1.0 1,285,810 - Pf6N2E2_1098 0.81 +1.5 1,285,905 + -0.3 1,285,905 + -1.5 1,285,926 + +0.1 1,285,926 + -0.3 1,285,926 + +0.8 1,285,927 - -0.3 1,285,944 + -0.3 1,285,944 + +0.1 1,285,985 + +1.3 1,286,005 + +1.9 1,286,006 - +0.3 1,286,158 + Pf6N2E2_1099 0.31 +1.9 1,286,193 - Pf6N2E2_1099 0.39 -1.7 1,286,237 + Pf6N2E2_1099 0.50 -0.3 1,286,238 - Pf6N2E2_1099 0.50 -0.7 1,286,460 - -0.6 1,286,460 - +1.7 1,286,498 + -0.2 1,286,498 + +0.5 1,286,510 + -0.8 1,286,511 - +0.0 1,286,511 - +0.4 1,286,713 + Pf6N2E2_1100 0.10 -0.1 1,286,714 - Pf6N2E2_1100 0.10 +0.6 1,286,737 + Pf6N2E2_1100 0.13 +0.2 1,286,737 + Pf6N2E2_1100 0.13 -0.2 1,286,737 + Pf6N2E2_1100 0.13 +0.0 1,286,738 - Pf6N2E2_1100 0.13 -0.3 1,286,738 - Pf6N2E2_1100 0.13 +0.9 1,286,738 - Pf6N2E2_1100 0.13 -0.0 1,286,802 + Pf6N2E2_1100 0.21 -0.6 1,286,802 + Pf6N2E2_1100 0.21 +0.0 1,286,878 + Pf6N2E2_1100 0.30 -1.5 1,286,878 + Pf6N2E2_1100 0.30 -0.8 1,286,890 + Pf6N2E2_1100 0.31 +0.4 1,287,073 - Pf6N2E2_1100 0.53 +0.4 1,287,286 + Pf6N2E2_1100 0.78 +0.7 1,287,287 - Pf6N2E2_1100 0.79 -0.6 1,287,287 - Pf6N2E2_1100 0.79 -0.1 1,287,287 - Pf6N2E2_1100 0.79 +0.2 1,287,287 - Pf6N2E2_1100 0.79 +0.9 1,287,287 - Pf6N2E2_1100 0.79 -1.7 1,287,287 - Pf6N2E2_1100 0.79 +0.5 1,287,434 - -1.2
Or see this region's nucleotide sequence