Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_01585 and AO356_01590 are separated by 13 nucleotides AO356_01590 and AO356_01595 overlap by 4 nucleotides
AO356_01585: AO356_01585 - 3-methylcrotonyl-CoA carboxylase, beta subunit (EC 6.4.1.4) <small>(from data)</small>, at 357,745 to 359,352
_01585
AO356_01590: AO356_01590 - Methylglutaconyl-CoA hydratase (EC 4.2.1.18) <small>(from data)</small>, at 359,366 to 360,187
_01590
AO356_01595: AO356_01595 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) <small>(from data)</small>, at 360,184 to 362,133
_01595
Position (kb) 359
360
361 Strain fitness (log2 ratio) -2
-1
0
1 at 358.487 kb on - strand, within AO356_01585 at 358.487 kb on - strand, within AO356_01585 at 358.487 kb on - strand, within AO356_01585 at 358.487 kb on - strand, within AO356_01585 at 358.594 kb on + strand, within AO356_01585 at 358.612 kb on + strand, within AO356_01585 at 358.612 kb on + strand, within AO356_01585 at 358.612 kb on + strand, within AO356_01585 at 358.613 kb on - strand, within AO356_01585 at 358.613 kb on - strand, within AO356_01585 at 358.897 kb on + strand, within AO356_01585 at 358.897 kb on + strand, within AO356_01585 at 358.898 kb on - strand, within AO356_01585 at 359.005 kb on - strand, within AO356_01585 at 359.008 kb on + strand, within AO356_01585 at 359.009 kb on - strand, within AO356_01585 at 359.029 kb on + strand, within AO356_01585 at 359.197 kb on + strand at 359.197 kb on + strand at 359.197 kb on + strand at 359.219 kb on - strand at 359.219 kb on - strand at 359.221 kb on + strand at 359.221 kb on + strand at 359.221 kb on + strand at 359.222 kb on - strand at 359.222 kb on - strand at 359.279 kb on + strand at 359.280 kb on - strand at 359.280 kb on - strand at 359.351 kb on - strand at 359.351 kb on - strand at 359.351 kb on - strand at 359.513 kb on - strand, within AO356_01590 at 359.603 kb on + strand, within AO356_01590 at 359.648 kb on + strand, within AO356_01590 at 359.648 kb on + strand, within AO356_01590 at 359.648 kb on + strand, within AO356_01590 at 359.648 kb on + strand, within AO356_01590 at 359.648 kb on + strand, within AO356_01590 at 359.648 kb on + strand, within AO356_01590 at 359.648 kb on + strand, within AO356_01590 at 359.649 kb on - strand, within AO356_01590 at 359.649 kb on - strand, within AO356_01590 at 359.722 kb on + strand, within AO356_01590 at 359.722 kb on + strand, within AO356_01590 at 359.723 kb on - strand, within AO356_01590 at 359.974 kb on - strand, within AO356_01590 at 359.974 kb on - strand, within AO356_01590 at 360.060 kb on - strand, within AO356_01590 at 360.164 kb on - strand at 360.287 kb on + strand at 360.373 kb on + strand at 360.373 kb on + strand at 360.374 kb on - strand at 360.374 kb on - strand at 360.799 kb on + strand, within AO356_01595 at 360.862 kb on + strand, within AO356_01595 at 360.997 kb on + strand, within AO356_01595 at 360.997 kb on + strand, within AO356_01595 at 360.998 kb on - strand, within AO356_01595
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 358,487 - AO356_01585 0.46 -1.4 358,487 - AO356_01585 0.46 -0.7 358,487 - AO356_01585 0.46 -1.3 358,487 - AO356_01585 0.46 +0.0 358,594 + AO356_01585 0.53 -0.9 358,612 + AO356_01585 0.54 +0.8 358,612 + AO356_01585 0.54 -0.8 358,612 + AO356_01585 0.54 +0.4 358,613 - AO356_01585 0.54 -1.7 358,613 - AO356_01585 0.54 -0.8 358,897 + AO356_01585 0.72 +0.1 358,897 + AO356_01585 0.72 -0.8 358,898 - AO356_01585 0.72 -0.8 359,005 - AO356_01585 0.78 -0.4 359,008 + AO356_01585 0.79 -1.2 359,009 - AO356_01585 0.79 -0.6 359,029 + AO356_01585 0.80 -2.0 359,197 + -0.1 359,197 + +0.6 359,197 + -0.2 359,219 - -0.6 359,219 - +0.5 359,221 + +0.2 359,221 + -1.5 359,221 + -0.2 359,222 - -0.7 359,222 - -0.1 359,279 + -1.6 359,280 - -1.5 359,280 - -1.3 359,351 - -1.0 359,351 - -0.5 359,351 - -1.9 359,513 - AO356_01590 0.18 -0.0 359,603 + AO356_01590 0.29 -1.1 359,648 + AO356_01590 0.34 -1.0 359,648 + AO356_01590 0.34 -0.5 359,648 + AO356_01590 0.34 -0.1 359,648 + AO356_01590 0.34 -2.3 359,648 + AO356_01590 0.34 -1.2 359,648 + AO356_01590 0.34 -1.5 359,648 + AO356_01590 0.34 -1.9 359,649 - AO356_01590 0.34 -0.3 359,649 - AO356_01590 0.34 -1.3 359,722 + AO356_01590 0.43 +0.8 359,722 + AO356_01590 0.43 -2.2 359,723 - AO356_01590 0.43 +0.2 359,974 - AO356_01590 0.74 +1.4 359,974 - AO356_01590 0.74 -1.0 360,060 - AO356_01590 0.84 +0.1 360,164 - -0.5 360,287 + -1.9 360,373 + -0.6 360,373 + -1.2 360,374 - -1.7 360,374 - -1.3 360,799 + AO356_01595 0.32 +0.1 360,862 + AO356_01595 0.35 +0.5 360,997 + AO356_01595 0.42 -0.8 360,997 + AO356_01595 0.42 -0.7 360,998 - AO356_01595 0.42 -0.7
Or see this region's nucleotide sequence