Experiment: thiosulfate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5591 and Pf6N2E2_5592 are separated by 469 nucleotides Pf6N2E2_5592 and Pf6N2E2_5593 are separated by 55 nucleotides Pf6N2E2_5593 and Pf6N2E2_5594 are separated by 107 nucleotides Pf6N2E2_5594 and Pf6N2E2_5595 overlap by 4 nucleotides
Pf6N2E2_5591 - hypothetical protein, at 6,408,785 to 6,409,267
_5591
Pf6N2E2_5592 - DNA-binding response regulator, LuxR family, at 6,409,737 to 6,410,363
_5592
Pf6N2E2_5593 - Leucyl-tRNA synthetase, at 6,410,419 to 6,410,601
_5593
Pf6N2E2_5594 - Endonuclease III (EC 4.2.99.18), at 6,410,709 to 6,411,347
_5594
Pf6N2E2_5595 - Electron transport complex protein RnfE, at 6,411,344 to 6,411,832
_5595
Position (kb)
6409
6410
6411 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6408.825 kb on + strand at 6408.825 kb on + strand at 6408.825 kb on + strand at 6408.825 kb on + strand at 6408.826 kb on - strand at 6408.826 kb on - strand at 6408.826 kb on - strand at 6408.826 kb on - strand at 6408.826 kb on - strand at 6408.834 kb on + strand, within Pf6N2E2_5591 at 6408.834 kb on + strand, within Pf6N2E2_5591 at 6408.834 kb on + strand, within Pf6N2E2_5591 at 6408.834 kb on + strand, within Pf6N2E2_5591 at 6408.835 kb on - strand, within Pf6N2E2_5591 at 6408.835 kb on - strand, within Pf6N2E2_5591 at 6408.905 kb on + strand, within Pf6N2E2_5591 at 6408.905 kb on + strand, within Pf6N2E2_5591 at 6408.905 kb on + strand, within Pf6N2E2_5591 at 6408.905 kb on + strand, within Pf6N2E2_5591 at 6408.910 kb on + strand, within Pf6N2E2_5591 at 6408.910 kb on + strand, within Pf6N2E2_5591 at 6408.911 kb on - strand, within Pf6N2E2_5591 at 6408.920 kb on - strand, within Pf6N2E2_5591 at 6408.920 kb on - strand, within Pf6N2E2_5591 at 6408.950 kb on + strand, within Pf6N2E2_5591 at 6408.950 kb on + strand, within Pf6N2E2_5591 at 6408.951 kb on - strand, within Pf6N2E2_5591 at 6408.951 kb on - strand, within Pf6N2E2_5591 at 6408.951 kb on - strand, within Pf6N2E2_5591 at 6408.986 kb on + strand, within Pf6N2E2_5591 at 6408.987 kb on - strand, within Pf6N2E2_5591 at 6408.987 kb on - strand, within Pf6N2E2_5591 at 6408.987 kb on - strand, within Pf6N2E2_5591 at 6408.987 kb on - strand, within Pf6N2E2_5591 at 6408.987 kb on - strand, within Pf6N2E2_5591 at 6409.029 kb on - strand, within Pf6N2E2_5591 at 6409.149 kb on + strand, within Pf6N2E2_5591 at 6409.217 kb on + strand, within Pf6N2E2_5591 at 6409.217 kb on + strand, within Pf6N2E2_5591 at 6409.271 kb on + strand at 6409.385 kb on + strand at 6409.392 kb on + strand at 6409.396 kb on + strand at 6409.491 kb on + strand at 6409.619 kb on + strand at 6409.619 kb on + strand at 6409.626 kb on + strand at 6409.627 kb on - strand at 6409.677 kb on - strand at 6409.691 kb on + strand at 6409.786 kb on + strand at 6409.812 kb on + strand, within Pf6N2E2_5592 at 6409.871 kb on + strand, within Pf6N2E2_5592 at 6410.034 kb on + strand, within Pf6N2E2_5592 at 6410.035 kb on - strand, within Pf6N2E2_5592 at 6410.035 kb on - strand, within Pf6N2E2_5592 at 6410.035 kb on - strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.057 kb on + strand, within Pf6N2E2_5592 at 6410.068 kb on + strand, within Pf6N2E2_5592 at 6410.068 kb on + strand, within Pf6N2E2_5592 at 6410.068 kb on + strand, within Pf6N2E2_5592 at 6410.069 kb on - strand, within Pf6N2E2_5592 at 6410.126 kb on + strand, within Pf6N2E2_5592 at 6410.276 kb on + strand, within Pf6N2E2_5592 at 6410.280 kb on + strand, within Pf6N2E2_5592 at 6410.280 kb on + strand, within Pf6N2E2_5592 at 6410.280 kb on + strand, within Pf6N2E2_5592 at 6410.281 kb on - strand, within Pf6N2E2_5592 at 6410.380 kb on + strand at 6410.380 kb on + strand at 6410.387 kb on + strand at 6410.413 kb on + strand at 6410.572 kb on + strand, within Pf6N2E2_5593 at 6410.572 kb on + strand, within Pf6N2E2_5593 at 6410.572 kb on + strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.618 kb on + strand at 6410.619 kb on - strand at 6410.640 kb on + strand at 6410.640 kb on + strand at 6410.640 kb on + strand at 6410.640 kb on + strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.735 kb on - strand at 6410.742 kb on + strand at 6410.743 kb on - strand at 6411.124 kb on + strand, within Pf6N2E2_5594 at 6411.124 kb on + strand, within Pf6N2E2_5594 at 6411.124 kb on + strand, within Pf6N2E2_5594 at 6411.154 kb on + strand, within Pf6N2E2_5594 at 6411.154 kb on + strand, within Pf6N2E2_5594 at 6411.154 kb on + strand, within Pf6N2E2_5594 at 6411.154 kb on + strand, within Pf6N2E2_5594 at 6411.155 kb on - strand, within Pf6N2E2_5594 at 6411.155 kb on - strand, within Pf6N2E2_5594 at 6411.171 kb on - strand, within Pf6N2E2_5594 at 6411.171 kb on - strand, within Pf6N2E2_5594 at 6411.171 kb on - strand, within Pf6N2E2_5594 at 6411.272 kb on - strand, within Pf6N2E2_5594 at 6411.321 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction thiosulfate 0.25 M remove 6,408,825 + +0.2 6,408,825 + -0.3 6,408,825 + +1.5 6,408,825 + +0.5 6,408,826 - +0.8 6,408,826 - -0.1 6,408,826 - +1.0 6,408,826 - -0.1 6,408,826 - +0.2 6,408,834 + Pf6N2E2_5591 0.10 +1.2 6,408,834 + Pf6N2E2_5591 0.10 -1.1 6,408,834 + Pf6N2E2_5591 0.10 +1.3 6,408,834 + Pf6N2E2_5591 0.10 +0.0 6,408,835 - Pf6N2E2_5591 0.10 +1.7 6,408,835 - Pf6N2E2_5591 0.10 +0.4 6,408,905 + Pf6N2E2_5591 0.25 +1.6 6,408,905 + Pf6N2E2_5591 0.25 -0.2 6,408,905 + Pf6N2E2_5591 0.25 -0.2 6,408,905 + Pf6N2E2_5591 0.25 -0.2 6,408,910 + Pf6N2E2_5591 0.26 -0.4 6,408,910 + Pf6N2E2_5591 0.26 +0.2 6,408,911 - Pf6N2E2_5591 0.26 -0.3 6,408,920 - Pf6N2E2_5591 0.28 +0.2 6,408,920 - Pf6N2E2_5591 0.28 -0.5 6,408,950 + Pf6N2E2_5591 0.34 +0.4 6,408,950 + Pf6N2E2_5591 0.34 +0.3 6,408,951 - Pf6N2E2_5591 0.34 +1.2 6,408,951 - Pf6N2E2_5591 0.34 -0.6 6,408,951 - Pf6N2E2_5591 0.34 -0.3 6,408,986 + Pf6N2E2_5591 0.42 +0.5 6,408,987 - Pf6N2E2_5591 0.42 +0.7 6,408,987 - Pf6N2E2_5591 0.42 -0.5 6,408,987 - Pf6N2E2_5591 0.42 -1.6 6,408,987 - Pf6N2E2_5591 0.42 -1.6 6,408,987 - Pf6N2E2_5591 0.42 +0.4 6,409,029 - Pf6N2E2_5591 0.51 -1.3 6,409,149 + Pf6N2E2_5591 0.75 +1.2 6,409,217 + Pf6N2E2_5591 0.89 +0.4 6,409,217 + Pf6N2E2_5591 0.89 -1.2 6,409,271 + +3.2 6,409,385 + +0.1 6,409,392 + -0.2 6,409,396 + -1.8 6,409,491 + -2.5 6,409,619 + +1.7 6,409,619 + -0.4 6,409,626 + -0.8 6,409,627 - -0.2 6,409,677 - +0.9 6,409,691 + +1.7 6,409,786 + +0.4 6,409,812 + Pf6N2E2_5592 0.12 +0.2 6,409,871 + Pf6N2E2_5592 0.21 +0.0 6,410,034 + Pf6N2E2_5592 0.47 +0.2 6,410,035 - Pf6N2E2_5592 0.48 -1.2 6,410,035 - Pf6N2E2_5592 0.48 -0.4 6,410,035 - Pf6N2E2_5592 0.48 +0.4 6,410,038 + Pf6N2E2_5592 0.48 +0.4 6,410,038 + Pf6N2E2_5592 0.48 +0.0 6,410,038 + Pf6N2E2_5592 0.48 -0.4 6,410,038 + Pf6N2E2_5592 0.48 -0.9 6,410,038 + Pf6N2E2_5592 0.48 -0.2 6,410,038 + Pf6N2E2_5592 0.48 -1.5 6,410,038 + Pf6N2E2_5592 0.48 +0.0 6,410,039 - Pf6N2E2_5592 0.48 +0.5 6,410,039 - Pf6N2E2_5592 0.48 -1.5 6,410,039 - Pf6N2E2_5592 0.48 -0.5 6,410,039 - Pf6N2E2_5592 0.48 +0.1 6,410,039 - Pf6N2E2_5592 0.48 +0.5 6,410,057 + Pf6N2E2_5592 0.51 -1.1 6,410,068 + Pf6N2E2_5592 0.53 -2.9 6,410,068 + Pf6N2E2_5592 0.53 -0.7 6,410,068 + Pf6N2E2_5592 0.53 +0.0 6,410,069 - Pf6N2E2_5592 0.53 +0.8 6,410,126 + Pf6N2E2_5592 0.62 +0.8 6,410,276 + Pf6N2E2_5592 0.86 -0.4 6,410,280 + Pf6N2E2_5592 0.87 -1.5 6,410,280 + Pf6N2E2_5592 0.87 -1.2 6,410,280 + Pf6N2E2_5592 0.87 -1.4 6,410,281 - Pf6N2E2_5592 0.87 +1.3 6,410,380 + +0.2 6,410,380 + +1.1 6,410,387 + -0.5 6,410,413 + +1.4 6,410,572 + Pf6N2E2_5593 0.84 -0.6 6,410,572 + Pf6N2E2_5593 0.84 -0.7 6,410,572 + Pf6N2E2_5593 0.84 +0.1 6,410,573 - Pf6N2E2_5593 0.84 +0.7 6,410,573 - Pf6N2E2_5593 0.84 -1.7 6,410,573 - Pf6N2E2_5593 0.84 -0.8 6,410,573 - Pf6N2E2_5593 0.84 +1.0 6,410,573 - Pf6N2E2_5593 0.84 -0.3 6,410,618 + +1.8 6,410,619 - +0.7 6,410,640 + +0.8 6,410,640 + -0.6 6,410,640 + +0.7 6,410,640 + -0.1 6,410,641 - -0.0 6,410,641 - +2.5 6,410,641 - +0.6 6,410,641 - +1.7 6,410,641 - +0.4 6,410,641 - +0.2 6,410,641 - +0.4 6,410,641 - -0.5 6,410,641 - +0.8 6,410,735 - +1.0 6,410,742 + -0.5 6,410,743 - -1.7 6,411,124 + Pf6N2E2_5594 0.65 +2.2 6,411,124 + Pf6N2E2_5594 0.65 +0.0 6,411,124 + Pf6N2E2_5594 0.65 -0.8 6,411,154 + Pf6N2E2_5594 0.70 -0.9 6,411,154 + Pf6N2E2_5594 0.70 +1.0 6,411,154 + Pf6N2E2_5594 0.70 -3.0 6,411,154 + Pf6N2E2_5594 0.70 -0.3 6,411,155 - Pf6N2E2_5594 0.70 -0.9 6,411,155 - Pf6N2E2_5594 0.70 -1.2 6,411,171 - Pf6N2E2_5594 0.72 -0.5 6,411,171 - Pf6N2E2_5594 0.72 -0.6 6,411,171 - Pf6N2E2_5594 0.72 -0.6 6,411,272 - Pf6N2E2_5594 0.88 -0.8 6,411,321 + +0.2
Or see this region's nucleotide sequence