Strain Fitness in Rhodanobacter denitrificans FW104-10B01 around LRK54_RS16615

Experiment: D-Raffinose 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntLRK54_RS16605 and LRK54_RS16610 overlap by 4 nucleotidesLRK54_RS16610 and LRK54_RS16615 are separated by 62 nucleotidesLRK54_RS16615 and LRK54_RS16620 are separated by 10 nucleotidesLRK54_RS16620 and LRK54_RS16625 overlap by 4 nucleotides LRK54_RS16605: LRK54_RS16605 - CTP synthase, at 3,647,509 to 3,649,179 _RS16605 LRK54_RS16610: LRK54_RS16610 - 3-deoxy-8-phosphooctulonate synthase, at 3,649,176 to 3,650,012 _RS16610 LRK54_RS16615: LRK54_RS16615 - phosphopyruvate hydratase, at 3,650,075 to 3,651,373 _RS16615 LRK54_RS16620: LRK54_RS16620 - cell division protein FtsB, at 3,651,384 to 3,651,719 _RS16620 LRK54_RS16625: LRK54_RS16625 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 3,651,716 to 3,652,414 _RS16625 Position (kb) 3650 3651 3652Strain fitness (log2 ratio) -2 -1 0 1at 3650.474 kb on + strand, within LRK54_RS16615at 3650.577 kb on + strand, within LRK54_RS16615at 3650.768 kb on + strand, within LRK54_RS16615at 3650.864 kb on + strand, within LRK54_RS16615at 3650.864 kb on + strand, within LRK54_RS16615at 3651.152 kb on + strand, within LRK54_RS16615at 3651.152 kb on + strand, within LRK54_RS16615at 3651.152 kb on + strand, within LRK54_RS16615at 3651.326 kb on + strandat 3651.326 kb on + strandat 3651.326 kb on + strandat 3651.326 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Raffinose 20 mM
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3,650,474 + LRK54_RS16615 0.31 -1.1
3,650,577 + LRK54_RS16615 0.39 -2.1
3,650,768 + LRK54_RS16615 0.53 -0.6
3,650,864 + LRK54_RS16615 0.61 -0.4
3,650,864 + LRK54_RS16615 0.61 -1.5
3,651,152 + LRK54_RS16615 0.83 +1.4
3,651,152 + LRK54_RS16615 0.83 -0.6
3,651,152 + LRK54_RS16615 0.83 +0.4
3,651,326 + -1.2
3,651,326 + +0.4
3,651,326 + -0.4
3,651,326 + -0.4

Or see this region's nucleotide sequence