Experiment: D-Raffinose 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS16605 and LRK54_RS16610 overlap by 4 nucleotides LRK54_RS16610 and LRK54_RS16615 are separated by 62 nucleotides LRK54_RS16615 and LRK54_RS16620 are separated by 10 nucleotides LRK54_RS16620 and LRK54_RS16625 overlap by 4 nucleotides
LRK54_RS16605: LRK54_RS16605 - CTP synthase, at 3,647,509 to 3,649,179
_RS16605
LRK54_RS16610: LRK54_RS16610 - 3-deoxy-8-phosphooctulonate synthase, at 3,649,176 to 3,650,012
_RS16610
LRK54_RS16615: LRK54_RS16615 - phosphopyruvate hydratase, at 3,650,075 to 3,651,373
_RS16615
LRK54_RS16620: LRK54_RS16620 - cell division protein FtsB, at 3,651,384 to 3,651,719
_RS16620
LRK54_RS16625: LRK54_RS16625 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 3,651,716 to 3,652,414
_RS16625
Position (kb)
3650
3651
3652 Strain fitness (log2 ratio)
-2
-1
0
1 at 3650.474 kb on + strand, within LRK54_RS16615 at 3650.577 kb on + strand, within LRK54_RS16615 at 3650.768 kb on + strand, within LRK54_RS16615 at 3650.864 kb on + strand, within LRK54_RS16615 at 3650.864 kb on + strand, within LRK54_RS16615 at 3651.152 kb on + strand, within LRK54_RS16615 at 3651.152 kb on + strand, within LRK54_RS16615 at 3651.152 kb on + strand, within LRK54_RS16615 at 3651.326 kb on + strand at 3651.326 kb on + strand at 3651.326 kb on + strand at 3651.326 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Raffinose 20 mM remove 3,650,474 + LRK54_RS16615 0.31 -1.1 3,650,577 + LRK54_RS16615 0.39 -2.1 3,650,768 + LRK54_RS16615 0.53 -0.6 3,650,864 + LRK54_RS16615 0.61 -0.4 3,650,864 + LRK54_RS16615 0.61 -1.5 3,651,152 + LRK54_RS16615 0.83 +1.4 3,651,152 + LRK54_RS16615 0.83 -0.6 3,651,152 + LRK54_RS16615 0.83 +0.4 3,651,326 + -1.2 3,651,326 + +0.4 3,651,326 + -0.4 3,651,326 + -0.4
Or see this region's nucleotide sequence