Experiment: LB 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2761 and Shew_2762 are separated by 91 nucleotides Shew_2762 and Shew_2763 overlap by 8 nucleotides Shew_2763 and Shew_2764 are separated by 60 nucleotides
Shew_2761: Shew_2761 - putative RNA 2'-O-ribose methyltransferase (RefSeq), at 3,266,754 to 3,267,845
_2761
Shew_2762: Shew_2762 - hypothetical protein (RefSeq), at 3,267,937 to 3,268,326
_2762
Shew_2763: Shew_2763 - dienelactone hydrolase (RefSeq), at 3,268,319 to 3,269,041
_2763
Shew_2764: Shew_2764 - DNA-binding transcriptional activator GcvA (RefSeq), at 3,269,102 to 3,270,013
_2764
Position (kb)
3268
3269
3270 Strain fitness (log2 ratio)
-2
-1
0
1 at 3267.402 kb on + strand, within Shew_2761 at 3267.567 kb on - strand, within Shew_2761 at 3267.709 kb on - strand, within Shew_2761 at 3267.900 kb on - strand at 3268.332 kb on - strand at 3268.392 kb on - strand, within Shew_2763 at 3268.671 kb on + strand, within Shew_2763 at 3268.938 kb on + strand, within Shew_2763 at 3269.551 kb on + strand, within Shew_2764 at 3269.551 kb on + strand, within Shew_2764
Per-strain Table
Position Strand Gene LocusTag Fraction LB 30C remove 3,267,402 + Shew_2761 0.59 -0.2 3,267,567 - Shew_2761 0.74 +0.1 3,267,709 - Shew_2761 0.87 +0.4 3,267,900 - +0.4 3,268,332 - +0.0 3,268,392 - Shew_2763 0.10 +0.2 3,268,671 + Shew_2763 0.49 +0.7 3,268,938 + Shew_2763 0.86 -0.0 3,269,551 + Shew_2764 0.49 -2.5 3,269,551 + Shew_2764 0.49 -2.7
Or see this region's nucleotide sequence