Experiment: LB 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1260 and Shew_1261 are separated by 5 nucleotides Shew_1261 and Shew_1262 are separated by 140 nucleotides Shew_1262 and Shew_1263 are separated by 147 nucleotides
Shew_1260: Shew_1260 - DNA methylase N-4/N-6 domain-containing protein (RefSeq), at 1,455,597 to 1,457,756
_1260
Shew_1261: Shew_1261 - type III restriction enzyme, res subunit (RefSeq), at 1,457,762 to 1,460,347
_1261
Shew_1262: Shew_1262 - hypothetical protein (RefSeq), at 1,460,488 to 1,460,853
_1262
Shew_1263: Shew_1263 - hypothetical protein (RefSeq), at 1,461,001 to 1,461,984
_1263
Position (kb)
1457
1458
1459
1460
1461 Strain fitness (log2 ratio)
-1
0
1 at 1456.837 kb on + strand, within Shew_1260 at 1457.340 kb on - strand, within Shew_1260 at 1457.692 kb on + strand at 1457.884 kb on + strand at 1458.270 kb on + strand, within Shew_1261 at 1458.851 kb on + strand, within Shew_1261 at 1459.171 kb on + strand, within Shew_1261 at 1459.629 kb on - strand, within Shew_1261 at 1460.445 kb on - strand at 1461.019 kb on + strand at 1461.066 kb on - strand at 1461.076 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 30C remove 1,456,837 + Shew_1260 0.57 +0.3 1,457,340 - Shew_1260 0.81 +0.0 1,457,692 + +0.4 1,457,884 + +0.2 1,458,270 + Shew_1261 0.20 +0.2 1,458,851 + Shew_1261 0.42 +0.6 1,459,171 + Shew_1261 0.54 +0.1 1,459,629 - Shew_1261 0.72 +0.0 1,460,445 - +0.4 1,461,019 + +0.1 1,461,066 - +0.4 1,461,076 - +0.0
Or see this region's nucleotide sequence