Experiment: LB 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0530 and Shew_0531 are separated by 62 nucleotides Shew_0531 and Shew_0532 are separated by 247 nucleotides
Shew_0530: Shew_0530 - FAD dependent oxidoreductase (RefSeq), at 599,363 to 600,490
_0530
Shew_0531: Shew_0531 - DEAD/DEAH box helicase domain-containing protein (RefSeq), at 600,553 to 602,352
_0531
Shew_0532: Shew_0532 - peptidase M28 (RefSeq), at 602,600 to 603,628
_0532
Position (kb)
600
601
602
603 Strain fitness (log2 ratio)
-1
0
1 at 599.562 kb on - strand, within Shew_0530 at 599.566 kb on + strand, within Shew_0530 at 599.819 kb on - strand, within Shew_0530 at 599.993 kb on - strand, within Shew_0530 at 600.012 kb on + strand, within Shew_0530 at 600.678 kb on - strand at 601.070 kb on + strand, within Shew_0531 at 601.078 kb on - strand, within Shew_0531 at 601.294 kb on - strand, within Shew_0531 at 601.999 kb on - strand, within Shew_0531 at 602.191 kb on - strand at 602.362 kb on - strand at 602.363 kb on - strand at 602.879 kb on - strand, within Shew_0532 at 602.879 kb on - strand, within Shew_0532 at 602.982 kb on + strand, within Shew_0532 at 602.982 kb on + strand, within Shew_0532 at 602.990 kb on - strand, within Shew_0532 at 602.990 kb on - strand, within Shew_0532 at 603.018 kb on + strand, within Shew_0532 at 603.112 kb on - strand, within Shew_0532
Per-strain Table
Position Strand Gene LocusTag Fraction LB 30C remove 599,562 - Shew_0530 0.18 +0.1 599,566 + Shew_0530 0.18 +0.2 599,819 - Shew_0530 0.40 +0.1 599,993 - Shew_0530 0.56 +0.7 600,012 + Shew_0530 0.58 -0.1 600,678 - -0.1 601,070 + Shew_0531 0.29 -0.9 601,078 - Shew_0531 0.29 -0.9 601,294 - Shew_0531 0.41 -0.2 601,999 - Shew_0531 0.80 -1.1 602,191 - -0.6 602,362 - -1.3 602,363 - +0.0 602,879 - Shew_0532 0.27 +0.3 602,879 - Shew_0532 0.27 -0.1 602,982 + Shew_0532 0.37 -0.1 602,982 + Shew_0532 0.37 +0.0 602,990 - Shew_0532 0.38 +0.3 602,990 - Shew_0532 0.38 +0.5 603,018 + Shew_0532 0.41 +0.1 603,112 - Shew_0532 0.50 -0.3
Or see this region's nucleotide sequence