Experiment: rubidium chloride 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4027 and Pf6N2E2_4028 are separated by 67 nucleotides Pf6N2E2_4028 and Pf6N2E2_4029 overlap by 4 nucleotides Pf6N2E2_4029 and Pf6N2E2_4030 are separated by 1 nucleotides
Pf6N2E2_4027 - Ferredoxin reductase, at 4,698,874 to 4,700,406
_4027
Pf6N2E2_4028 - Biofilm PGA synthesis auxiliary protein PgaD, at 4,700,474 to 4,700,980
_4028
Pf6N2E2_4029 - Biofilm PGA synthesis N-glycosyltransferase PgaC (EC 2.4.-.-), at 4,700,977 to 4,702,332
_4029
Pf6N2E2_4030 - Biofilm PGA synthesis deacetylase PgaB (EC 3.-), at 4,702,334 to 4,704,331
_4030
Position (kb)
4700
4701
4702
4703 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4700.021 kb on + strand, within Pf6N2E2_4027 at 4700.147 kb on - strand, within Pf6N2E2_4027 at 4700.173 kb on + strand, within Pf6N2E2_4027 at 4700.173 kb on + strand, within Pf6N2E2_4027 at 4700.173 kb on + strand, within Pf6N2E2_4027 at 4700.173 kb on + strand, within Pf6N2E2_4027 at 4700.173 kb on + strand, within Pf6N2E2_4027 at 4700.174 kb on - strand, within Pf6N2E2_4027 at 4700.174 kb on - strand, within Pf6N2E2_4027 at 4700.191 kb on + strand, within Pf6N2E2_4027 at 4700.192 kb on - strand, within Pf6N2E2_4027 at 4700.197 kb on + strand, within Pf6N2E2_4027 at 4700.197 kb on + strand, within Pf6N2E2_4027 at 4700.197 kb on + strand, within Pf6N2E2_4027 at 4700.197 kb on + strand, within Pf6N2E2_4027 at 4700.197 kb on + strand, within Pf6N2E2_4027 at 4700.198 kb on - strand, within Pf6N2E2_4027 at 4700.198 kb on - strand, within Pf6N2E2_4027 at 4700.198 kb on - strand, within Pf6N2E2_4027 at 4700.262 kb on + strand at 4700.262 kb on + strand at 4700.262 kb on + strand at 4700.262 kb on + strand at 4700.263 kb on - strand at 4700.404 kb on + strand at 4700.404 kb on + strand at 4700.404 kb on + strand at 4700.404 kb on + strand at 4700.405 kb on - strand at 4700.405 kb on - strand at 4700.480 kb on + strand at 4700.480 kb on + strand at 4700.481 kb on - strand at 4700.481 kb on - strand at 4700.481 kb on - strand at 4700.614 kb on + strand, within Pf6N2E2_4028 at 4700.614 kb on + strand, within Pf6N2E2_4028 at 4700.614 kb on + strand, within Pf6N2E2_4028 at 4700.614 kb on + strand, within Pf6N2E2_4028 at 4700.614 kb on + strand, within Pf6N2E2_4028 at 4700.614 kb on + strand, within Pf6N2E2_4028 at 4700.615 kb on - strand, within Pf6N2E2_4028 at 4700.615 kb on - strand, within Pf6N2E2_4028 at 4700.615 kb on - strand, within Pf6N2E2_4028 at 4700.615 kb on - strand, within Pf6N2E2_4028 at 4700.615 kb on - strand, within Pf6N2E2_4028 at 4700.737 kb on + strand, within Pf6N2E2_4028 at 4700.799 kb on + strand, within Pf6N2E2_4028 at 4700.799 kb on + strand, within Pf6N2E2_4028 at 4700.800 kb on - strand, within Pf6N2E2_4028 at 4700.800 kb on - strand, within Pf6N2E2_4028 at 4700.800 kb on - strand, within Pf6N2E2_4028 at 4700.895 kb on + strand, within Pf6N2E2_4028 at 4700.895 kb on + strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.896 kb on - strand, within Pf6N2E2_4028 at 4700.918 kb on + strand, within Pf6N2E2_4028 at 4700.919 kb on - strand, within Pf6N2E2_4028 at 4700.919 kb on - strand, within Pf6N2E2_4028 at 4700.919 kb on - strand, within Pf6N2E2_4028 at 4700.972 kb on - strand at 4701.068 kb on - strand at 4701.068 kb on - strand at 4701.068 kb on - strand at 4701.356 kb on + strand, within Pf6N2E2_4029 at 4701.380 kb on + strand, within Pf6N2E2_4029 at 4701.518 kb on + strand, within Pf6N2E2_4029 at 4701.519 kb on - strand, within Pf6N2E2_4029 at 4701.519 kb on - strand, within Pf6N2E2_4029 at 4701.727 kb on + strand, within Pf6N2E2_4029 at 4701.810 kb on - strand, within Pf6N2E2_4029 at 4701.860 kb on + strand, within Pf6N2E2_4029 at 4701.860 kb on + strand, within Pf6N2E2_4029 at 4701.861 kb on - strand, within Pf6N2E2_4029 at 4702.181 kb on + strand, within Pf6N2E2_4029 at 4702.181 kb on + strand, within Pf6N2E2_4029 at 4702.182 kb on - strand, within Pf6N2E2_4029 at 4702.182 kb on - strand, within Pf6N2E2_4029 at 4702.410 kb on + strand at 4702.410 kb on + strand at 4702.413 kb on + strand at 4702.435 kb on + strand at 4702.436 kb on - strand at 4702.436 kb on - strand at 4702.891 kb on - strand, within Pf6N2E2_4030 at 4702.891 kb on - strand, within Pf6N2E2_4030 at 4702.951 kb on - strand, within Pf6N2E2_4030 at 4702.992 kb on + strand, within Pf6N2E2_4030 at 4702.992 kb on + strand, within Pf6N2E2_4030 at 4702.993 kb on - strand, within Pf6N2E2_4030 at 4703.046 kb on + strand, within Pf6N2E2_4030 at 4703.046 kb on + strand, within Pf6N2E2_4030 at 4703.046 kb on + strand, within Pf6N2E2_4030 at 4703.047 kb on - strand, within Pf6N2E2_4030 at 4703.047 kb on - strand, within Pf6N2E2_4030 at 4703.148 kb on + strand, within Pf6N2E2_4030 at 4703.148 kb on + strand, within Pf6N2E2_4030 at 4703.148 kb on + strand, within Pf6N2E2_4030 at 4703.148 kb on + strand, within Pf6N2E2_4030 at 4703.148 kb on + strand, within Pf6N2E2_4030 at 4703.149 kb on - strand, within Pf6N2E2_4030 at 4703.236 kb on - strand, within Pf6N2E2_4030 at 4703.236 kb on - strand, within Pf6N2E2_4030 at 4703.236 kb on - strand, within Pf6N2E2_4030 at 4703.236 kb on - strand, within Pf6N2E2_4030 at 4703.293 kb on + strand, within Pf6N2E2_4030 at 4703.293 kb on + strand, within Pf6N2E2_4030 at 4703.294 kb on - strand, within Pf6N2E2_4030 at 4703.294 kb on - strand, within Pf6N2E2_4030 at 4703.314 kb on + strand, within Pf6N2E2_4030 at 4703.314 kb on + strand, within Pf6N2E2_4030
Per-strain Table
Position Strand Gene LocusTag Fraction rubidium chloride 0.25 M remove 4,700,021 + Pf6N2E2_4027 0.75 +0.2 4,700,147 - Pf6N2E2_4027 0.83 +0.4 4,700,173 + Pf6N2E2_4027 0.85 +0.1 4,700,173 + Pf6N2E2_4027 0.85 +0.5 4,700,173 + Pf6N2E2_4027 0.85 -1.4 4,700,173 + Pf6N2E2_4027 0.85 +0.5 4,700,173 + Pf6N2E2_4027 0.85 -0.1 4,700,174 - Pf6N2E2_4027 0.85 +1.2 4,700,174 - Pf6N2E2_4027 0.85 -0.8 4,700,191 + Pf6N2E2_4027 0.86 +0.0 4,700,192 - Pf6N2E2_4027 0.86 +0.2 4,700,197 + Pf6N2E2_4027 0.86 +0.2 4,700,197 + Pf6N2E2_4027 0.86 -0.7 4,700,197 + Pf6N2E2_4027 0.86 +0.8 4,700,197 + Pf6N2E2_4027 0.86 -0.3 4,700,197 + Pf6N2E2_4027 0.86 +1.5 4,700,198 - Pf6N2E2_4027 0.86 -0.1 4,700,198 - Pf6N2E2_4027 0.86 +0.4 4,700,198 - Pf6N2E2_4027 0.86 +0.5 4,700,262 + +1.6 4,700,262 + +0.0 4,700,262 + -0.1 4,700,262 + -0.2 4,700,263 - +0.1 4,700,404 + +0.2 4,700,404 + +0.6 4,700,404 + -0.2 4,700,404 + -0.8 4,700,405 - -0.1 4,700,405 - -0.5 4,700,480 + -0.6 4,700,480 + -2.6 4,700,481 - -0.1 4,700,481 - -1.3 4,700,481 - +1.0 4,700,614 + Pf6N2E2_4028 0.28 +0.3 4,700,614 + Pf6N2E2_4028 0.28 +0.1 4,700,614 + Pf6N2E2_4028 0.28 -1.3 4,700,614 + Pf6N2E2_4028 0.28 +0.5 4,700,614 + Pf6N2E2_4028 0.28 +0.1 4,700,614 + Pf6N2E2_4028 0.28 -0.9 4,700,615 - Pf6N2E2_4028 0.28 +0.4 4,700,615 - Pf6N2E2_4028 0.28 -1.2 4,700,615 - Pf6N2E2_4028 0.28 -0.5 4,700,615 - Pf6N2E2_4028 0.28 -1.2 4,700,615 - Pf6N2E2_4028 0.28 -0.3 4,700,737 + Pf6N2E2_4028 0.52 -0.1 4,700,799 + Pf6N2E2_4028 0.64 -0.1 4,700,799 + Pf6N2E2_4028 0.64 +1.6 4,700,800 - Pf6N2E2_4028 0.64 +0.4 4,700,800 - Pf6N2E2_4028 0.64 +0.3 4,700,800 - Pf6N2E2_4028 0.64 +0.2 4,700,895 + Pf6N2E2_4028 0.83 +1.0 4,700,895 + Pf6N2E2_4028 0.83 -0.8 4,700,896 - Pf6N2E2_4028 0.83 +0.0 4,700,896 - Pf6N2E2_4028 0.83 -0.8 4,700,896 - Pf6N2E2_4028 0.83 -0.4 4,700,896 - Pf6N2E2_4028 0.83 +0.1 4,700,896 - Pf6N2E2_4028 0.83 -0.7 4,700,896 - Pf6N2E2_4028 0.83 +0.7 4,700,896 - Pf6N2E2_4028 0.83 +0.3 4,700,896 - Pf6N2E2_4028 0.83 -0.6 4,700,896 - Pf6N2E2_4028 0.83 +0.5 4,700,896 - Pf6N2E2_4028 0.83 +0.0 4,700,918 + Pf6N2E2_4028 0.88 -1.5 4,700,919 - Pf6N2E2_4028 0.88 +1.4 4,700,919 - Pf6N2E2_4028 0.88 -0.6 4,700,919 - Pf6N2E2_4028 0.88 -1.3 4,700,972 - -0.6 4,701,068 - +0.5 4,701,068 - -0.5 4,701,068 - +0.2 4,701,356 + Pf6N2E2_4029 0.28 +1.0 4,701,380 + Pf6N2E2_4029 0.30 -0.3 4,701,518 + Pf6N2E2_4029 0.40 +0.0 4,701,519 - Pf6N2E2_4029 0.40 +0.9 4,701,519 - Pf6N2E2_4029 0.40 +0.3 4,701,727 + Pf6N2E2_4029 0.55 -1.0 4,701,810 - Pf6N2E2_4029 0.61 +0.8 4,701,860 + Pf6N2E2_4029 0.65 -1.4 4,701,860 + Pf6N2E2_4029 0.65 +0.5 4,701,861 - Pf6N2E2_4029 0.65 +0.6 4,702,181 + Pf6N2E2_4029 0.89 -0.3 4,702,181 + Pf6N2E2_4029 0.89 +0.2 4,702,182 - Pf6N2E2_4029 0.89 -0.7 4,702,182 - Pf6N2E2_4029 0.89 -0.1 4,702,410 + +1.2 4,702,410 + -0.7 4,702,413 + -0.4 4,702,435 + -0.7 4,702,436 - -0.1 4,702,436 - +0.5 4,702,891 - Pf6N2E2_4030 0.28 +0.8 4,702,891 - Pf6N2E2_4030 0.28 +3.3 4,702,951 - Pf6N2E2_4030 0.31 +1.3 4,702,992 + Pf6N2E2_4030 0.33 -1.3 4,702,992 + Pf6N2E2_4030 0.33 +2.4 4,702,993 - Pf6N2E2_4030 0.33 +0.1 4,703,046 + Pf6N2E2_4030 0.36 -0.6 4,703,046 + Pf6N2E2_4030 0.36 +1.0 4,703,046 + Pf6N2E2_4030 0.36 +0.1 4,703,047 - Pf6N2E2_4030 0.36 -0.2 4,703,047 - Pf6N2E2_4030 0.36 -0.5 4,703,148 + Pf6N2E2_4030 0.41 +1.4 4,703,148 + Pf6N2E2_4030 0.41 -0.2 4,703,148 + Pf6N2E2_4030 0.41 -1.7 4,703,148 + Pf6N2E2_4030 0.41 -0.2 4,703,148 + Pf6N2E2_4030 0.41 +0.9 4,703,149 - Pf6N2E2_4030 0.41 -0.6 4,703,236 - Pf6N2E2_4030 0.45 -0.5 4,703,236 - Pf6N2E2_4030 0.45 -1.9 4,703,236 - Pf6N2E2_4030 0.45 +0.4 4,703,236 - Pf6N2E2_4030 0.45 +0.6 4,703,293 + Pf6N2E2_4030 0.48 +2.4 4,703,293 + Pf6N2E2_4030 0.48 +0.8 4,703,294 - Pf6N2E2_4030 0.48 +0.7 4,703,294 - Pf6N2E2_4030 0.48 +0.2 4,703,314 + Pf6N2E2_4030 0.49 -0.2 4,703,314 + Pf6N2E2_4030 0.49 -0.5
Or see this region's nucleotide sequence