Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3514 and Shew_3515 overlap by 8 nucleotides Shew_3515 and Shew_3516 overlap by 1 nucleotides
Shew_3514: Shew_3514 - hypothetical protein (RefSeq), at 4,199,705 to 4,201,066
_3514
Shew_3515: Shew_3515 - Beta-hydroxyacyl-(acyl-carrier-protein) dehydratase, FabA/FabZ (RefSeq), at 4,201,059 to 4,201,430
_3515
Shew_3516: Shew_3516 - glycosyl transferase family protein (RefSeq), at 4,201,430 to 4,203,148
_3516
Position (kb)
4201
4202 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6
7
8
9
10 at 4200.090 kb on + strand, within Shew_3514 at 4200.124 kb on + strand, within Shew_3514 at 4200.124 kb on + strand, within Shew_3514 at 4200.132 kb on - strand, within Shew_3514 at 4200.178 kb on + strand, within Shew_3514 at 4200.273 kb on - strand, within Shew_3514 at 4200.841 kb on + strand, within Shew_3514 at 4200.841 kb on + strand, within Shew_3514 at 4200.849 kb on - strand, within Shew_3514 at 4201.145 kb on + strand, within Shew_3515 at 4201.258 kb on + strand, within Shew_3515 at 4201.266 kb on - strand, within Shew_3515 at 4201.266 kb on - strand, within Shew_3515 at 4201.266 kb on - strand, within Shew_3515 at 4201.266 kb on - strand, within Shew_3515 at 4201.366 kb on - strand, within Shew_3515 at 4201.618 kb on - strand, within Shew_3516 at 4201.750 kb on + strand, within Shew_3516 at 4201.849 kb on + strand, within Shew_3516 at 4201.865 kb on - strand, within Shew_3516 at 4202.179 kb on - strand, within Shew_3516 at 4202.235 kb on + strand, within Shew_3516 at 4202.235 kb on + strand, within Shew_3516 at 4202.243 kb on - strand, within Shew_3516 at 4202.243 kb on - strand, within Shew_3516 at 4202.406 kb on + strand, within Shew_3516
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 4,200,090 + Shew_3514 0.28 +6.7 4,200,124 + Shew_3514 0.31 +7.5 4,200,124 + Shew_3514 0.31 +6.2 4,200,132 - Shew_3514 0.31 +6.7 4,200,178 + Shew_3514 0.35 +7.1 4,200,273 - Shew_3514 0.42 +5.1 4,200,841 + Shew_3514 0.83 +6.4 4,200,841 + Shew_3514 0.83 +6.6 4,200,849 - Shew_3514 0.84 +7.2 4,201,145 + Shew_3515 0.23 +7.2 4,201,258 + Shew_3515 0.53 +6.7 4,201,266 - Shew_3515 0.56 +6.4 4,201,266 - Shew_3515 0.56 +6.7 4,201,266 - Shew_3515 0.56 +6.5 4,201,266 - Shew_3515 0.56 +7.4 4,201,366 - Shew_3515 0.83 +6.4 4,201,618 - Shew_3516 0.11 +4.7 4,201,750 + Shew_3516 0.19 +6.6 4,201,849 + Shew_3516 0.24 +6.5 4,201,865 - Shew_3516 0.25 +7.2 4,202,179 - Shew_3516 0.44 +6.3 4,202,235 + Shew_3516 0.47 +6.7 4,202,235 + Shew_3516 0.47 +6.8 4,202,243 - Shew_3516 0.47 +10.3 4,202,243 - Shew_3516 0.47 +6.7 4,202,406 + Shew_3516 0.57 +6.7
Or see this region's nucleotide sequence