Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3221 and Pf6N2E2_3222 are separated by 108 nucleotides Pf6N2E2_3222 and Pf6N2E2_3223 are separated by 29 nucleotides Pf6N2E2_3223 and Pf6N2E2_3224 are separated by 129 nucleotides Pf6N2E2_3224 and Pf6N2E2_3225 are separated by 181 nucleotides
Pf6N2E2_3221 - FIG00352444: hypothetical protein, at 3,727,429 to 3,727,974
_3221
Pf6N2E2_3222 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 3,728,083 to 3,728,844
_3222
Pf6N2E2_3223 - uncharacterized protein SCO0976, at 3,728,874 to 3,729,506
_3223
Pf6N2E2_3224 - Lactoylglutathione lyase and related lyases, at 3,729,636 to 3,730,052
_3224
Pf6N2E2_3225 - hypothetical protein, at 3,730,234 to 3,731,283
_3225
Position (kb)
3728
3729
3730 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3728.027 kb on + strand at 3728.027 kb on + strand at 3728.031 kb on + strand at 3728.031 kb on + strand at 3728.031 kb on + strand at 3728.031 kb on + strand at 3728.031 kb on + strand at 3728.032 kb on - strand at 3728.032 kb on - strand at 3728.032 kb on - strand at 3728.036 kb on + strand at 3728.043 kb on - strand at 3728.125 kb on - strand at 3728.273 kb on + strand, within Pf6N2E2_3222 at 3728.273 kb on + strand, within Pf6N2E2_3222 at 3728.325 kb on - strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.397 kb on - strand, within Pf6N2E2_3222 at 3728.397 kb on - strand, within Pf6N2E2_3222 at 3728.397 kb on - strand, within Pf6N2E2_3222 at 3728.397 kb on - strand, within Pf6N2E2_3222 at 3728.397 kb on - strand, within Pf6N2E2_3222 at 3728.397 kb on - strand, within Pf6N2E2_3222 at 3728.397 kb on - strand, within Pf6N2E2_3222 at 3728.397 kb on - strand, within Pf6N2E2_3222 at 3728.399 kb on + strand, within Pf6N2E2_3222 at 3728.399 kb on + strand, within Pf6N2E2_3222 at 3728.399 kb on + strand, within Pf6N2E2_3222 at 3728.399 kb on + strand, within Pf6N2E2_3222 at 3728.399 kb on + strand, within Pf6N2E2_3222 at 3728.399 kb on + strand, within Pf6N2E2_3222 at 3728.400 kb on - strand, within Pf6N2E2_3222 at 3728.400 kb on - strand, within Pf6N2E2_3222 at 3728.462 kb on + strand, within Pf6N2E2_3222 at 3728.462 kb on + strand, within Pf6N2E2_3222 at 3728.474 kb on + strand, within Pf6N2E2_3222 at 3728.493 kb on - strand, within Pf6N2E2_3222 at 3728.496 kb on + strand, within Pf6N2E2_3222 at 3728.497 kb on - strand, within Pf6N2E2_3222 at 3728.497 kb on - strand, within Pf6N2E2_3222 at 3728.676 kb on + strand, within Pf6N2E2_3222 at 3728.676 kb on + strand, within Pf6N2E2_3222 at 3728.676 kb on + strand, within Pf6N2E2_3222 at 3728.677 kb on - strand, within Pf6N2E2_3222 at 3728.677 kb on - strand, within Pf6N2E2_3222 at 3728.677 kb on - strand, within Pf6N2E2_3222 at 3728.677 kb on - strand, within Pf6N2E2_3222 at 3728.720 kb on + strand, within Pf6N2E2_3222 at 3728.720 kb on + strand, within Pf6N2E2_3222 at 3728.779 kb on + strand at 3728.837 kb on + strand at 3728.876 kb on - strand at 3728.876 kb on - strand at 3729.091 kb on + strand, within Pf6N2E2_3223 at 3729.091 kb on + strand, within Pf6N2E2_3223 at 3729.091 kb on + strand, within Pf6N2E2_3223 at 3729.092 kb on - strand, within Pf6N2E2_3223 at 3729.092 kb on - strand, within Pf6N2E2_3223 at 3729.092 kb on - strand, within Pf6N2E2_3223 at 3729.092 kb on - strand, within Pf6N2E2_3223 at 3729.092 kb on - strand, within Pf6N2E2_3223 at 3729.158 kb on + strand, within Pf6N2E2_3223 at 3729.158 kb on + strand, within Pf6N2E2_3223 at 3729.158 kb on + strand, within Pf6N2E2_3223 at 3729.159 kb on - strand, within Pf6N2E2_3223 at 3729.159 kb on - strand, within Pf6N2E2_3223 at 3729.159 kb on - strand, within Pf6N2E2_3223 at 3729.302 kb on + strand, within Pf6N2E2_3223 at 3729.303 kb on - strand, within Pf6N2E2_3223 at 3729.330 kb on + strand, within Pf6N2E2_3223 at 3729.331 kb on - strand, within Pf6N2E2_3223 at 3729.331 kb on - strand, within Pf6N2E2_3223 at 3729.338 kb on - strand, within Pf6N2E2_3223 at 3729.507 kb on - strand at 3729.531 kb on + strand at 3729.531 kb on + strand at 3729.531 kb on + strand at 3729.532 kb on - strand at 3729.532 kb on - strand at 3729.532 kb on - strand at 3729.603 kb on + strand at 3729.604 kb on - strand at 3729.635 kb on + strand at 3729.659 kb on + strand at 3729.660 kb on - strand at 3729.660 kb on - strand at 3729.683 kb on - strand, within Pf6N2E2_3224 at 3729.712 kb on - strand, within Pf6N2E2_3224 at 3729.712 kb on - strand, within Pf6N2E2_3224 at 3729.713 kb on + strand, within Pf6N2E2_3224 at 3729.713 kb on + strand, within Pf6N2E2_3224 at 3729.714 kb on - strand, within Pf6N2E2_3224 at 3729.993 kb on + strand, within Pf6N2E2_3224 at 3730.103 kb on - strand at 3730.289 kb on + strand at 3730.290 kb on - strand at 3730.290 kb on - strand at 3730.441 kb on - strand, within Pf6N2E2_3225 at 3730.441 kb on - strand, within Pf6N2E2_3225 at 3730.441 kb on - strand, within Pf6N2E2_3225 at 3730.441 kb on - strand, within Pf6N2E2_3225 at 3730.441 kb on - strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.444 kb on - strand, within Pf6N2E2_3225 at 3730.444 kb on - strand, within Pf6N2E2_3225 at 3730.444 kb on - strand, within Pf6N2E2_3225
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 3,728,027 + -1.6 3,728,027 + +1.1 3,728,031 + +1.4 3,728,031 + +1.0 3,728,031 + -1.6 3,728,031 + -0.9 3,728,031 + -2.0 3,728,032 - -1.3 3,728,032 - +0.3 3,728,032 - +0.7 3,728,036 + -2.6 3,728,043 - +1.0 3,728,125 - +1.5 3,728,273 + Pf6N2E2_3222 0.25 +0.9 3,728,273 + Pf6N2E2_3222 0.25 +0.2 3,728,325 - Pf6N2E2_3222 0.32 -0.1 3,728,396 + Pf6N2E2_3222 0.41 +0.8 3,728,396 + Pf6N2E2_3222 0.41 -2.5 3,728,396 + Pf6N2E2_3222 0.41 +1.4 3,728,396 + Pf6N2E2_3222 0.41 +1.0 3,728,396 + Pf6N2E2_3222 0.41 +0.8 3,728,396 + Pf6N2E2_3222 0.41 +0.5 3,728,396 + Pf6N2E2_3222 0.41 +1.2 3,728,396 + Pf6N2E2_3222 0.41 +1.5 3,728,396 + Pf6N2E2_3222 0.41 -2.7 3,728,396 + Pf6N2E2_3222 0.41 -0.1 3,728,397 - Pf6N2E2_3222 0.41 -3.0 3,728,397 - Pf6N2E2_3222 0.41 -0.9 3,728,397 - Pf6N2E2_3222 0.41 -0.7 3,728,397 - Pf6N2E2_3222 0.41 -2.1 3,728,397 - Pf6N2E2_3222 0.41 +0.2 3,728,397 - Pf6N2E2_3222 0.41 +1.4 3,728,397 - Pf6N2E2_3222 0.41 +0.1 3,728,397 - Pf6N2E2_3222 0.41 +0.1 3,728,399 + Pf6N2E2_3222 0.41 -2.1 3,728,399 + Pf6N2E2_3222 0.41 -0.0 3,728,399 + Pf6N2E2_3222 0.41 +0.6 3,728,399 + Pf6N2E2_3222 0.41 +1.0 3,728,399 + Pf6N2E2_3222 0.41 +1.7 3,728,399 + Pf6N2E2_3222 0.41 -2.4 3,728,400 - Pf6N2E2_3222 0.42 +1.3 3,728,400 - Pf6N2E2_3222 0.42 +0.7 3,728,462 + Pf6N2E2_3222 0.50 +1.0 3,728,462 + Pf6N2E2_3222 0.50 +0.6 3,728,474 + Pf6N2E2_3222 0.51 -2.7 3,728,493 - Pf6N2E2_3222 0.54 -1.5 3,728,496 + Pf6N2E2_3222 0.54 -1.6 3,728,497 - Pf6N2E2_3222 0.54 +1.4 3,728,497 - Pf6N2E2_3222 0.54 +1.2 3,728,676 + Pf6N2E2_3222 0.78 +0.0 3,728,676 + Pf6N2E2_3222 0.78 +1.3 3,728,676 + Pf6N2E2_3222 0.78 +1.0 3,728,677 - Pf6N2E2_3222 0.78 +0.5 3,728,677 - Pf6N2E2_3222 0.78 +0.2 3,728,677 - Pf6N2E2_3222 0.78 -0.9 3,728,677 - Pf6N2E2_3222 0.78 +1.9 3,728,720 + Pf6N2E2_3222 0.84 +2.5 3,728,720 + Pf6N2E2_3222 0.84 +0.4 3,728,779 + +0.5 3,728,837 + -1.1 3,728,876 - -0.7 3,728,876 - +1.2 3,729,091 + Pf6N2E2_3223 0.34 +0.5 3,729,091 + Pf6N2E2_3223 0.34 -1.1 3,729,091 + Pf6N2E2_3223 0.34 -1.1 3,729,092 - Pf6N2E2_3223 0.34 -0.8 3,729,092 - Pf6N2E2_3223 0.34 -2.1 3,729,092 - Pf6N2E2_3223 0.34 -2.1 3,729,092 - Pf6N2E2_3223 0.34 -0.2 3,729,092 - Pf6N2E2_3223 0.34 -2.5 3,729,158 + Pf6N2E2_3223 0.45 +0.9 3,729,158 + Pf6N2E2_3223 0.45 -1.1 3,729,158 + Pf6N2E2_3223 0.45 -1.5 3,729,159 - Pf6N2E2_3223 0.45 +1.4 3,729,159 - Pf6N2E2_3223 0.45 -2.9 3,729,159 - Pf6N2E2_3223 0.45 -0.2 3,729,302 + Pf6N2E2_3223 0.68 -0.8 3,729,303 - Pf6N2E2_3223 0.68 -0.8 3,729,330 + Pf6N2E2_3223 0.72 -1.9 3,729,331 - Pf6N2E2_3223 0.72 +0.6 3,729,331 - Pf6N2E2_3223 0.72 +0.9 3,729,338 - Pf6N2E2_3223 0.73 +2.6 3,729,507 - +1.6 3,729,531 + +0.5 3,729,531 + -1.2 3,729,531 + -1.2 3,729,532 - -0.0 3,729,532 - -2.1 3,729,532 - -1.4 3,729,603 + +0.0 3,729,604 - -0.3 3,729,635 + +1.2 3,729,659 + +1.8 3,729,660 - +2.3 3,729,660 - +1.7 3,729,683 - Pf6N2E2_3224 0.11 -0.2 3,729,712 - Pf6N2E2_3224 0.18 -2.0 3,729,712 - Pf6N2E2_3224 0.18 +2.2 3,729,713 + Pf6N2E2_3224 0.18 +2.2 3,729,713 + Pf6N2E2_3224 0.18 -0.2 3,729,714 - Pf6N2E2_3224 0.19 -1.8 3,729,993 + Pf6N2E2_3224 0.86 +0.7 3,730,103 - +0.4 3,730,289 + +1.2 3,730,290 - -1.3 3,730,290 - +1.1 3,730,441 - Pf6N2E2_3225 0.20 -2.8 3,730,441 - Pf6N2E2_3225 0.20 -0.5 3,730,441 - Pf6N2E2_3225 0.20 +0.2 3,730,441 - Pf6N2E2_3225 0.20 +1.4 3,730,441 - Pf6N2E2_3225 0.20 +1.5 3,730,443 + Pf6N2E2_3225 0.20 +1.8 3,730,443 + Pf6N2E2_3225 0.20 -1.0 3,730,443 + Pf6N2E2_3225 0.20 -1.8 3,730,443 + Pf6N2E2_3225 0.20 +0.7 3,730,443 + Pf6N2E2_3225 0.20 +0.9 3,730,443 + Pf6N2E2_3225 0.20 +1.3 3,730,444 - Pf6N2E2_3225 0.20 +0.9 3,730,444 - Pf6N2E2_3225 0.20 -2.3 3,730,444 - Pf6N2E2_3225 0.20 +1.3
Or see this region's nucleotide sequence