Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_156 and PfGW456L13_157 overlap by 4 nucleotides PfGW456L13_157 and PfGW456L13_158 are separated by 15 nucleotides PfGW456L13_158 and PfGW456L13_159 overlap by 13 nucleotides
PfGW456L13_156 - DNA mismatch repair protein MutL, at 179,934 to 181,844
_156
PfGW456L13_157 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 181,841 to 183,256
_157
PfGW456L13_158 - TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 183,272 to 183,742
_158
PfGW456L13_159 - NAD(P)HX epimerase / NAD(P)HX dehydratase, at 183,730 to 185,229
_159
Position (kb) 181
182
183
184 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 180.857 kb on - strand, within PfGW456L13_156 at 181.061 kb on - strand, within PfGW456L13_156 at 181.113 kb on + strand, within PfGW456L13_156 at 181.114 kb on - strand, within PfGW456L13_156 at 181.114 kb on - strand, within PfGW456L13_156 at 181.114 kb on - strand, within PfGW456L13_156 at 181.114 kb on - strand, within PfGW456L13_156 at 182.046 kb on + strand, within PfGW456L13_157 at 182.047 kb on - strand, within PfGW456L13_157 at 182.047 kb on - strand, within PfGW456L13_157 at 182.047 kb on - strand, within PfGW456L13_157 at 183.257 kb on - strand at 183.257 kb on - strand at 183.274 kb on - strand at 183.274 kb on - strand at 183.274 kb on - strand at 183.274 kb on - strand at 183.274 kb on - strand at 184.030 kb on - strand, within PfGW456L13_159 at 184.030 kb on - strand, within PfGW456L13_159
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 180,857 - PfGW456L13_156 0.48 +0.4 181,061 - PfGW456L13_156 0.59 +0.6 181,113 + PfGW456L13_156 0.62 +0.6 181,114 - PfGW456L13_156 0.62 +0.4 181,114 - PfGW456L13_156 0.62 -0.7 181,114 - PfGW456L13_156 0.62 +0.3 181,114 - PfGW456L13_156 0.62 -0.2 182,046 + PfGW456L13_157 0.14 -1.1 182,047 - PfGW456L13_157 0.15 -3.1 182,047 - PfGW456L13_157 0.15 -1.6 182,047 - PfGW456L13_157 0.15 -1.2 183,257 - -1.6 183,257 - -0.5 183,274 - +2.2 183,274 - -0.6 183,274 - -0.2 183,274 - -1.3 183,274 - +0.6 184,030 - PfGW456L13_159 0.20 -0.9 184,030 - PfGW456L13_159 0.20 +0.7
Or see this region's nucleotide sequence