Experiment: pyrophosphate 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5772 and Pf6N2E2_5773 are separated by 90 nucleotides Pf6N2E2_5773 and Pf6N2E2_5774 overlap by 4 nucleotides Pf6N2E2_5774 and Pf6N2E2_5775 are separated by 0 nucleotides Pf6N2E2_5775 and Pf6N2E2_5776 are separated by 29 nucleotides Pf6N2E2_5776 and Pf6N2E2_5777 are separated by 45 nucleotides
Pf6N2E2_5772 - DUF1232 domain-containing protein, at 6,589,192 to 6,589,647
_5772
Pf6N2E2_5773 - Transcriptional regulator, Cro/CI family, at 6,589,738 to 6,590,103
_5773
Pf6N2E2_5774 - FIG00954549: hypothetical protein, at 6,590,100 to 6,590,471
_5774
Pf6N2E2_5775 - FOG: TPR repeat, SEL1 subfamily, at 6,590,472 to 6,590,873
_5775
Pf6N2E2_5776 - hypothetical protein, at 6,590,903 to 6,591,016
_5776
Pf6N2E2_5777 - Rhs family protein, at 6,591,062 to 6,595,720
_5777
Position (kb)
6590
6591 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6589.700 kb on + strand at 6589.700 kb on - strand at 6589.701 kb on - strand at 6589.701 kb on - strand at 6589.706 kb on + strand at 6589.714 kb on + strand at 6589.715 kb on - strand at 6589.735 kb on + strand at 6589.757 kb on + strand at 6589.932 kb on - strand, within Pf6N2E2_5773 at 6589.985 kb on + strand, within Pf6N2E2_5773 at 6589.985 kb on + strand, within Pf6N2E2_5773 at 6589.985 kb on + strand, within Pf6N2E2_5773 at 6589.986 kb on - strand, within Pf6N2E2_5773 at 6589.986 kb on - strand, within Pf6N2E2_5773 at 6589.986 kb on - strand, within Pf6N2E2_5773 at 6590.002 kb on + strand, within Pf6N2E2_5773 at 6590.002 kb on + strand, within Pf6N2E2_5773 at 6590.003 kb on - strand, within Pf6N2E2_5773 at 6590.107 kb on + strand at 6590.107 kb on + strand at 6590.107 kb on + strand at 6590.108 kb on - strand at 6590.176 kb on + strand, within Pf6N2E2_5774 at 6590.176 kb on + strand, within Pf6N2E2_5774 at 6590.176 kb on + strand, within Pf6N2E2_5774 at 6590.176 kb on + strand, within Pf6N2E2_5774 at 6590.177 kb on - strand, within Pf6N2E2_5774 at 6590.177 kb on - strand, within Pf6N2E2_5774 at 6590.177 kb on - strand, within Pf6N2E2_5774 at 6590.184 kb on + strand, within Pf6N2E2_5774 at 6590.185 kb on - strand, within Pf6N2E2_5774 at 6590.356 kb on + strand, within Pf6N2E2_5774 at 6590.356 kb on + strand, within Pf6N2E2_5774 at 6590.357 kb on - strand, within Pf6N2E2_5774 at 6590.357 kb on - strand, within Pf6N2E2_5774 at 6590.442 kb on + strand at 6590.443 kb on - strand at 6590.443 kb on - strand at 6590.443 kb on - strand at 6590.625 kb on + strand, within Pf6N2E2_5775 at 6590.625 kb on + strand, within Pf6N2E2_5775 at 6590.626 kb on - strand, within Pf6N2E2_5775 at 6590.626 kb on - strand, within Pf6N2E2_5775 at 6590.626 kb on - strand, within Pf6N2E2_5775 at 6590.634 kb on - strand, within Pf6N2E2_5775 at 6590.634 kb on - strand, within Pf6N2E2_5775 at 6590.866 kb on - strand at 6590.872 kb on - strand at 6590.915 kb on - strand, within Pf6N2E2_5776 at 6590.954 kb on + strand, within Pf6N2E2_5776 at 6590.954 kb on + strand, within Pf6N2E2_5776 at 6590.955 kb on - strand, within Pf6N2E2_5776 at 6590.955 kb on - strand, within Pf6N2E2_5776 at 6590.955 kb on - strand, within Pf6N2E2_5776 at 6590.955 kb on - strand, within Pf6N2E2_5776 at 6591.122 kb on + strand at 6591.290 kb on - strand at 6591.332 kb on + strand at 6591.332 kb on + strand at 6591.332 kb on + strand at 6591.333 kb on - strand at 6591.453 kb on - strand at 6591.512 kb on + strand at 6591.512 kb on + strand at 6591.512 kb on + strand at 6591.512 kb on + strand at 6591.512 kb on + strand at 6591.512 kb on + strand at 6591.854 kb on - strand, within Pf6N2E2_5777 at 6591.854 kb on - strand, within Pf6N2E2_5777 at 6591.854 kb on - strand, within Pf6N2E2_5777
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M remove 6,589,700 + +0.1 6,589,700 - -2.0 6,589,701 - -0.3 6,589,701 - -0.6 6,589,706 + +0.3 6,589,714 + +1.6 6,589,715 - -0.5 6,589,735 + +1.7 6,589,757 + -0.9 6,589,932 - Pf6N2E2_5773 0.53 +0.6 6,589,985 + Pf6N2E2_5773 0.67 -0.2 6,589,985 + Pf6N2E2_5773 0.67 +0.8 6,589,985 + Pf6N2E2_5773 0.67 +0.7 6,589,986 - Pf6N2E2_5773 0.68 +0.0 6,589,986 - Pf6N2E2_5773 0.68 -1.3 6,589,986 - Pf6N2E2_5773 0.68 -0.6 6,590,002 + Pf6N2E2_5773 0.72 +1.3 6,590,002 + Pf6N2E2_5773 0.72 +0.7 6,590,003 - Pf6N2E2_5773 0.72 -0.4 6,590,107 + +0.0 6,590,107 + +0.2 6,590,107 + -0.6 6,590,108 - +0.5 6,590,176 + Pf6N2E2_5774 0.20 -1.3 6,590,176 + Pf6N2E2_5774 0.20 -0.1 6,590,176 + Pf6N2E2_5774 0.20 -1.1 6,590,176 + Pf6N2E2_5774 0.20 +0.7 6,590,177 - Pf6N2E2_5774 0.21 -1.0 6,590,177 - Pf6N2E2_5774 0.21 -1.8 6,590,177 - Pf6N2E2_5774 0.21 -0.8 6,590,184 + Pf6N2E2_5774 0.23 -0.8 6,590,185 - Pf6N2E2_5774 0.23 -0.5 6,590,356 + Pf6N2E2_5774 0.69 -1.9 6,590,356 + Pf6N2E2_5774 0.69 -0.2 6,590,357 - Pf6N2E2_5774 0.69 -0.3 6,590,357 - Pf6N2E2_5774 0.69 +0.0 6,590,442 + -0.4 6,590,443 - -0.2 6,590,443 - -0.5 6,590,443 - -0.6 6,590,625 + Pf6N2E2_5775 0.38 -1.6 6,590,625 + Pf6N2E2_5775 0.38 +1.2 6,590,626 - Pf6N2E2_5775 0.38 +2.7 6,590,626 - Pf6N2E2_5775 0.38 -1.6 6,590,626 - Pf6N2E2_5775 0.38 -1.1 6,590,634 - Pf6N2E2_5775 0.40 -2.5 6,590,634 - Pf6N2E2_5775 0.40 -1.7 6,590,866 - -0.6 6,590,872 - -1.0 6,590,915 - Pf6N2E2_5776 0.11 -0.5 6,590,954 + Pf6N2E2_5776 0.45 +0.8 6,590,954 + Pf6N2E2_5776 0.45 -0.4 6,590,955 - Pf6N2E2_5776 0.46 -0.1 6,590,955 - Pf6N2E2_5776 0.46 -2.3 6,590,955 - Pf6N2E2_5776 0.46 +0.4 6,590,955 - Pf6N2E2_5776 0.46 +1.0 6,591,122 + -0.1 6,591,290 - +1.7 6,591,332 + +1.7 6,591,332 + +0.5 6,591,332 + +0.6 6,591,333 - -0.5 6,591,453 - +1.7 6,591,512 + +0.4 6,591,512 + -0.4 6,591,512 + +0.1 6,591,512 + +0.9 6,591,512 + -0.3 6,591,512 + -0.6 6,591,854 - Pf6N2E2_5777 0.17 -0.9 6,591,854 - Pf6N2E2_5777 0.17 -0.2 6,591,854 - Pf6N2E2_5777 0.17 -2.7
Or see this region's nucleotide sequence