Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4562

Experiment: pyrophosphate 0.0025 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4560 and Pf6N2E2_4561 are separated by 86 nucleotidesPf6N2E2_4561 and Pf6N2E2_4562 are separated by 144 nucleotidesPf6N2E2_4562 and Pf6N2E2_4563 are separated by 242 nucleotides Pf6N2E2_4560 - probable integral membrane protein NMA1899, at 5,288,951 to 5,290,318 _4560 Pf6N2E2_4561 - Dihydrofolate reductase (EC 1.5.1.3), at 5,290,405 to 5,290,917 _4561 Pf6N2E2_4562 - L-cystine uptake protein TcyP, at 5,291,062 to 5,292,453 _4562 Pf6N2E2_4563 - Phosphoserine phosphatase (EC 3.1.3.3), at 5,292,696 to 5,293,751 _4563 Position (kb) 5291 5292 5293Strain fitness (log2 ratio) -2 -1 0 1at 5290.202 kb on + strandat 5290.280 kb on + strandat 5290.310 kb on + strandat 5290.310 kb on + strandat 5290.353 kb on - strandat 5290.392 kb on + strandat 5290.926 kb on + strandat 5290.926 kb on + strandat 5290.926 kb on + strandat 5290.926 kb on + strandat 5290.926 kb on + strandat 5290.926 kb on + strandat 5290.927 kb on - strandat 5290.927 kb on - strandat 5290.927 kb on - strandat 5290.927 kb on - strandat 5290.927 kb on - strandat 5291.071 kb on + strandat 5291.651 kb on + strand, within Pf6N2E2_4562at 5291.651 kb on + strand, within Pf6N2E2_4562at 5291.994 kb on - strand, within Pf6N2E2_4562at 5292.303 kb on - strand, within Pf6N2E2_4562at 5292.303 kb on - strand, within Pf6N2E2_4562at 5292.341 kb on - strandat 5292.341 kb on - strandat 5292.514 kb on + strandat 5292.515 kb on - strandat 5292.515 kb on - strandat 5292.526 kb on - strandat 5292.530 kb on - strandat 5292.537 kb on + strandat 5292.555 kb on + strandat 5292.555 kb on + strandat 5292.556 kb on - strandat 5292.595 kb on + strandat 5292.837 kb on - strand, within Pf6N2E2_4563at 5292.840 kb on + strand, within Pf6N2E2_4563at 5292.840 kb on + strand, within Pf6N2E2_4563at 5292.840 kb on + strand, within Pf6N2E2_4563at 5292.840 kb on + strand, within Pf6N2E2_4563at 5292.841 kb on - strand, within Pf6N2E2_4563at 5292.841 kb on - strand, within Pf6N2E2_4563at 5292.841 kb on - strand, within Pf6N2E2_4563at 5292.841 kb on - strand, within Pf6N2E2_4563at 5292.841 kb on - strand, within Pf6N2E2_4563at 5292.841 kb on - strand, within Pf6N2E2_4563at 5292.841 kb on - strand, within Pf6N2E2_4563at 5292.870 kb on + strand, within Pf6N2E2_4563at 5292.871 kb on - strand, within Pf6N2E2_4563at 5293.005 kb on + strand, within Pf6N2E2_4563at 5293.005 kb on + strand, within Pf6N2E2_4563at 5293.005 kb on + strand, within Pf6N2E2_4563at 5293.006 kb on - strand, within Pf6N2E2_4563at 5293.006 kb on - strand, within Pf6N2E2_4563at 5293.008 kb on + strand, within Pf6N2E2_4563at 5293.008 kb on + strand, within Pf6N2E2_4563at 5293.009 kb on - strand, within Pf6N2E2_4563at 5293.009 kb on - strand, within Pf6N2E2_4563at 5293.009 kb on - strand, within Pf6N2E2_4563at 5293.044 kb on + strand, within Pf6N2E2_4563at 5293.099 kb on - strand, within Pf6N2E2_4563at 5293.099 kb on - strand, within Pf6N2E2_4563at 5293.099 kb on - strand, within Pf6N2E2_4563at 5293.099 kb on - strand, within Pf6N2E2_4563at 5293.143 kb on + strand, within Pf6N2E2_4563at 5293.143 kb on + strand, within Pf6N2E2_4563at 5293.143 kb on + strand, within Pf6N2E2_4563at 5293.143 kb on + strand, within Pf6N2E2_4563at 5293.143 kb on + strand, within Pf6N2E2_4563at 5293.143 kb on + strand, within Pf6N2E2_4563at 5293.143 kb on + strand, within Pf6N2E2_4563at 5293.143 kb on + strand, within Pf6N2E2_4563at 5293.144 kb on - strand, within Pf6N2E2_4563at 5293.144 kb on - strand, within Pf6N2E2_4563at 5293.144 kb on - strand, within Pf6N2E2_4563at 5293.144 kb on - strand, within Pf6N2E2_4563at 5293.144 kb on - strand, within Pf6N2E2_4563at 5293.146 kb on + strand, within Pf6N2E2_4563at 5293.146 kb on + strand, within Pf6N2E2_4563at 5293.146 kb on + strand, within Pf6N2E2_4563at 5293.146 kb on + strand, within Pf6N2E2_4563at 5293.146 kb on + strand, within Pf6N2E2_4563at 5293.146 kb on + strand, within Pf6N2E2_4563at 5293.147 kb on - strand, within Pf6N2E2_4563at 5293.147 kb on - strand, within Pf6N2E2_4563at 5293.147 kb on - strand, within Pf6N2E2_4563at 5293.170 kb on + strand, within Pf6N2E2_4563at 5293.170 kb on + strand, within Pf6N2E2_4563at 5293.170 kb on + strand, within Pf6N2E2_4563at 5293.171 kb on - strand, within Pf6N2E2_4563at 5293.215 kb on + strand, within Pf6N2E2_4563at 5293.215 kb on + strand, within Pf6N2E2_4563at 5293.215 kb on + strand, within Pf6N2E2_4563at 5293.215 kb on + strand, within Pf6N2E2_4563at 5293.216 kb on - strand, within Pf6N2E2_4563at 5293.216 kb on - strand, within Pf6N2E2_4563at 5293.216 kb on - strand, within Pf6N2E2_4563at 5293.216 kb on - strand, within Pf6N2E2_4563at 5293.216 kb on - strand, within Pf6N2E2_4563at 5293.216 kb on - strand, within Pf6N2E2_4563at 5293.248 kb on + strand, within Pf6N2E2_4563at 5293.248 kb on + strand, within Pf6N2E2_4563at 5293.248 kb on + strand, within Pf6N2E2_4563at 5293.248 kb on + strand, within Pf6N2E2_4563at 5293.249 kb on - strand, within Pf6N2E2_4563at 5293.249 kb on - strand, within Pf6N2E2_4563at 5293.249 kb on - strand, within Pf6N2E2_4563at 5293.249 kb on - strand, within Pf6N2E2_4563at 5293.249 kb on - strand, within Pf6N2E2_4563at 5293.249 kb on - strand, within Pf6N2E2_4563at 5293.249 kb on - strand, within Pf6N2E2_4563at 5293.249 kb on - strand, within Pf6N2E2_4563at 5293.249 kb on - strand, within Pf6N2E2_4563at 5293.311 kb on + strand, within Pf6N2E2_4563at 5293.312 kb on - strand, within Pf6N2E2_4563at 5293.413 kb on + strand, within Pf6N2E2_4563at 5293.414 kb on - strand, within Pf6N2E2_4563at 5293.443 kb on + strand, within Pf6N2E2_4563at 5293.444 kb on - strand, within Pf6N2E2_4563at 5293.453 kb on - strand, within Pf6N2E2_4563at 5293.453 kb on - strand, within Pf6N2E2_4563

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Per-strain Table

Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M
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5,290,202 + +0.4
5,290,280 + +1.5
5,290,310 + -0.6
5,290,310 + +0.8
5,290,353 - -0.3
5,290,392 + +0.1
5,290,926 + +0.9
5,290,926 + -0.5
5,290,926 + +0.0
5,290,926 + -1.0
5,290,926 + -0.1
5,290,926 + +0.6
5,290,927 - +0.8
5,290,927 - -1.4
5,290,927 - +0.4
5,290,927 - +0.8
5,290,927 - -0.1
5,291,071 + +0.9
5,291,651 + Pf6N2E2_4562 0.42 -0.3
5,291,651 + Pf6N2E2_4562 0.42 -1.7
5,291,994 - Pf6N2E2_4562 0.67 -0.4
5,292,303 - Pf6N2E2_4562 0.89 -1.5
5,292,303 - Pf6N2E2_4562 0.89 -0.0
5,292,341 - +0.4
5,292,341 - +0.4
5,292,514 + +0.5
5,292,515 - +0.1
5,292,515 - -0.6
5,292,526 - -0.4
5,292,530 - -0.1
5,292,537 + +1.2
5,292,555 + -0.5
5,292,555 + -0.5
5,292,556 - +0.4
5,292,595 + -1.7
5,292,837 - Pf6N2E2_4563 0.13 +0.5
5,292,840 + Pf6N2E2_4563 0.14 +0.0
5,292,840 + Pf6N2E2_4563 0.14 +0.8
5,292,840 + Pf6N2E2_4563 0.14 -0.5
5,292,840 + Pf6N2E2_4563 0.14 +0.4
5,292,841 - Pf6N2E2_4563 0.14 +0.0
5,292,841 - Pf6N2E2_4563 0.14 -0.5
5,292,841 - Pf6N2E2_4563 0.14 +0.2
5,292,841 - Pf6N2E2_4563 0.14 -0.3
5,292,841 - Pf6N2E2_4563 0.14 -0.3
5,292,841 - Pf6N2E2_4563 0.14 +0.2
5,292,841 - Pf6N2E2_4563 0.14 -0.7
5,292,870 + Pf6N2E2_4563 0.16 +1.0
5,292,871 - Pf6N2E2_4563 0.17 -0.3
5,293,005 + Pf6N2E2_4563 0.29 +1.2
5,293,005 + Pf6N2E2_4563 0.29 -0.5
5,293,005 + Pf6N2E2_4563 0.29 +1.1
5,293,006 - Pf6N2E2_4563 0.29 +0.8
5,293,006 - Pf6N2E2_4563 0.29 +0.3
5,293,008 + Pf6N2E2_4563 0.30 +0.5
5,293,008 + Pf6N2E2_4563 0.30 -2.2
5,293,009 - Pf6N2E2_4563 0.30 -0.5
5,293,009 - Pf6N2E2_4563 0.30 +0.4
5,293,009 - Pf6N2E2_4563 0.30 +0.3
5,293,044 + Pf6N2E2_4563 0.33 +1.3
5,293,099 - Pf6N2E2_4563 0.38 -0.3
5,293,099 - Pf6N2E2_4563 0.38 -0.1
5,293,099 - Pf6N2E2_4563 0.38 -0.1
5,293,099 - Pf6N2E2_4563 0.38 -1.8
5,293,143 + Pf6N2E2_4563 0.42 -0.7
5,293,143 + Pf6N2E2_4563 0.42 +0.2
5,293,143 + Pf6N2E2_4563 0.42 +0.8
5,293,143 + Pf6N2E2_4563 0.42 +1.2
5,293,143 + Pf6N2E2_4563 0.42 -0.1
5,293,143 + Pf6N2E2_4563 0.42 +0.5
5,293,143 + Pf6N2E2_4563 0.42 +0.9
5,293,143 + Pf6N2E2_4563 0.42 +0.8
5,293,144 - Pf6N2E2_4563 0.42 +0.0
5,293,144 - Pf6N2E2_4563 0.42 -0.9
5,293,144 - Pf6N2E2_4563 0.42 +1.3
5,293,144 - Pf6N2E2_4563 0.42 +1.2
5,293,144 - Pf6N2E2_4563 0.42 -0.4
5,293,146 + Pf6N2E2_4563 0.43 -0.9
5,293,146 + Pf6N2E2_4563 0.43 -1.1
5,293,146 + Pf6N2E2_4563 0.43 -1.1
5,293,146 + Pf6N2E2_4563 0.43 +0.2
5,293,146 + Pf6N2E2_4563 0.43 +0.8
5,293,146 + Pf6N2E2_4563 0.43 -0.0
5,293,147 - Pf6N2E2_4563 0.43 -0.2
5,293,147 - Pf6N2E2_4563 0.43 -1.8
5,293,147 - Pf6N2E2_4563 0.43 -0.4
5,293,170 + Pf6N2E2_4563 0.45 -0.6
5,293,170 + Pf6N2E2_4563 0.45 -0.0
5,293,170 + Pf6N2E2_4563 0.45 -1.2
5,293,171 - Pf6N2E2_4563 0.45 -1.9
5,293,215 + Pf6N2E2_4563 0.49 +0.1
5,293,215 + Pf6N2E2_4563 0.49 +0.5
5,293,215 + Pf6N2E2_4563 0.49 -0.4
5,293,215 + Pf6N2E2_4563 0.49 -0.1
5,293,216 - Pf6N2E2_4563 0.49 +1.5
5,293,216 - Pf6N2E2_4563 0.49 +0.3
5,293,216 - Pf6N2E2_4563 0.49 +1.2
5,293,216 - Pf6N2E2_4563 0.49 +1.2
5,293,216 - Pf6N2E2_4563 0.49 +0.6
5,293,216 - Pf6N2E2_4563 0.49 +0.6
5,293,248 + Pf6N2E2_4563 0.52 +0.4
5,293,248 + Pf6N2E2_4563 0.52 +0.4
5,293,248 + Pf6N2E2_4563 0.52 -0.2
5,293,248 + Pf6N2E2_4563 0.52 -1.2
5,293,249 - Pf6N2E2_4563 0.52 -0.7
5,293,249 - Pf6N2E2_4563 0.52 -0.2
5,293,249 - Pf6N2E2_4563 0.52 +0.5
5,293,249 - Pf6N2E2_4563 0.52 +1.2
5,293,249 - Pf6N2E2_4563 0.52 +0.6
5,293,249 - Pf6N2E2_4563 0.52 -0.2
5,293,249 - Pf6N2E2_4563 0.52 +1.3
5,293,249 - Pf6N2E2_4563 0.52 -0.5
5,293,249 - Pf6N2E2_4563 0.52 +0.4
5,293,311 + Pf6N2E2_4563 0.58 +0.2
5,293,312 - Pf6N2E2_4563 0.58 -0.4
5,293,413 + Pf6N2E2_4563 0.68 +1.0
5,293,414 - Pf6N2E2_4563 0.68 -0.7
5,293,443 + Pf6N2E2_4563 0.71 -0.5
5,293,444 - Pf6N2E2_4563 0.71 -0.2
5,293,453 - Pf6N2E2_4563 0.72 +1.7
5,293,453 - Pf6N2E2_4563 0.72 -0.2

Or see this region's nucleotide sequence