Experiment: pyrophosphate 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4419 and Pf6N2E2_4420 are separated by 9 nucleotides Pf6N2E2_4420 and Pf6N2E2_4421 overlap by 18 nucleotides Pf6N2E2_4421 and Pf6N2E2_4422 overlap by 4 nucleotides Pf6N2E2_4422 and Pf6N2E2_4423 are separated by 4 nucleotides Pf6N2E2_4423 and Pf6N2E2_4424 are separated by 300 nucleotides Pf6N2E2_4424 and Pf6N2E2_4425 are separated by 7 nucleotides
Pf6N2E2_4419 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 5,152,073 to 5,153,320
_4419
Pf6N2E2_4420 - Lipoprotein LppL-related protein, at 5,153,330 to 5,153,452
_4420
Pf6N2E2_4421 - hypothetical protein, at 5,153,435 to 5,153,692
_4421
Pf6N2E2_4422 - Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly, at 5,153,689 to 5,154,021
_4422
Pf6N2E2_4423 - FIG00953342: hypothetical protein, at 5,154,026 to 5,154,244
_4423
Pf6N2E2_4424 - Regulator of nucleoside diphosphate kinase, at 5,154,545 to 5,154,955
_4424
Pf6N2E2_4425 - Adenylate cyclase (EC 4.6.1.1), at 5,154,963 to 5,157,806
_4425
Position (kb)
5153
5154
5155 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5153.332 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.541 kb on - strand, within Pf6N2E2_4421 at 5153.541 kb on - strand, within Pf6N2E2_4421 at 5153.543 kb on - strand, within Pf6N2E2_4421 at 5153.566 kb on - strand, within Pf6N2E2_4421 at 5153.611 kb on + strand, within Pf6N2E2_4421 at 5153.612 kb on - strand, within Pf6N2E2_4421 at 5153.612 kb on - strand, within Pf6N2E2_4421 at 5153.613 kb on + strand, within Pf6N2E2_4421 at 5153.614 kb on - strand, within Pf6N2E2_4421 at 5153.614 kb on - strand, within Pf6N2E2_4421 at 5153.614 kb on - strand, within Pf6N2E2_4421 at 5153.642 kb on + strand, within Pf6N2E2_4421 at 5153.642 kb on + strand, within Pf6N2E2_4421 at 5153.643 kb on - strand, within Pf6N2E2_4421 at 5153.801 kb on + strand, within Pf6N2E2_4422 at 5153.801 kb on + strand, within Pf6N2E2_4422 at 5153.905 kb on + strand, within Pf6N2E2_4422 at 5153.906 kb on - strand, within Pf6N2E2_4422 at 5154.019 kb on + strand at 5154.065 kb on + strand, within Pf6N2E2_4423 at 5154.066 kb on - strand, within Pf6N2E2_4423 at 5154.066 kb on - strand, within Pf6N2E2_4423 at 5154.066 kb on - strand, within Pf6N2E2_4423 at 5154.205 kb on + strand, within Pf6N2E2_4423 at 5154.242 kb on + strand at 5154.242 kb on + strand at 5154.243 kb on - strand at 5154.396 kb on + strand at 5154.396 kb on + strand at 5154.396 kb on + strand at 5154.396 kb on + strand at 5154.396 kb on + strand at 5154.396 kb on + strand at 5154.397 kb on - strand at 5154.397 kb on - strand at 5154.397 kb on - strand at 5154.397 kb on - strand at 5154.397 kb on - strand at 5154.397 kb on - strand at 5154.397 kb on - strand at 5154.397 kb on - strand at 5154.397 kb on - strand at 5154.397 kb on - strand at 5154.397 kb on - strand at 5154.397 kb on - strand at 5154.397 kb on - strand at 5154.397 kb on - strand at 5154.397 kb on - strand at 5154.397 kb on - strand at 5154.400 kb on + strand at 5154.425 kb on + strand at 5154.425 kb on + strand at 5154.426 kb on - strand at 5154.426 kb on - strand at 5154.429 kb on + strand at 5154.429 kb on + strand at 5154.429 kb on + strand at 5154.430 kb on - strand at 5154.430 kb on - strand at 5154.430 kb on - strand at 5154.442 kb on + strand at 5154.442 kb on + strand at 5154.443 kb on - strand at 5154.443 kb on - strand at 5154.443 kb on - strand at 5154.582 kb on + strand at 5154.582 kb on + strand at 5154.582 kb on + strand at 5154.582 kb on + strand at 5154.583 kb on - strand at 5154.583 kb on - strand at 5154.583 kb on - strand at 5154.755 kb on + strand, within Pf6N2E2_4424 at 5154.755 kb on + strand, within Pf6N2E2_4424 at 5154.755 kb on + strand, within Pf6N2E2_4424 at 5154.755 kb on + strand, within Pf6N2E2_4424 at 5154.756 kb on - strand, within Pf6N2E2_4424 at 5154.756 kb on - strand, within Pf6N2E2_4424 at 5154.773 kb on + strand, within Pf6N2E2_4424 at 5155.012 kb on + strand at 5155.012 kb on + strand at 5155.013 kb on - strand at 5155.013 kb on - strand at 5155.021 kb on + strand at 5155.021 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M remove 5,153,332 - -1.2 5,153,335 - -0.8 5,153,335 - +1.1 5,153,335 - +0.1 5,153,335 - +0.1 5,153,335 - +0.4 5,153,335 - +0.4 5,153,335 - -1.2 5,153,335 - -0.1 5,153,335 - -0.7 5,153,335 - +0.1 5,153,541 - Pf6N2E2_4421 0.41 -0.2 5,153,541 - Pf6N2E2_4421 0.41 +0.9 5,153,543 - Pf6N2E2_4421 0.42 -0.1 5,153,566 - Pf6N2E2_4421 0.51 +0.1 5,153,611 + Pf6N2E2_4421 0.68 +0.7 5,153,612 - Pf6N2E2_4421 0.69 +0.3 5,153,612 - Pf6N2E2_4421 0.69 -0.1 5,153,613 + Pf6N2E2_4421 0.69 -1.1 5,153,614 - Pf6N2E2_4421 0.69 +0.3 5,153,614 - Pf6N2E2_4421 0.69 +0.8 5,153,614 - Pf6N2E2_4421 0.69 +0.3 5,153,642 + Pf6N2E2_4421 0.80 +0.7 5,153,642 + Pf6N2E2_4421 0.80 +0.8 5,153,643 - Pf6N2E2_4421 0.81 -1.3 5,153,801 + Pf6N2E2_4422 0.34 -1.4 5,153,801 + Pf6N2E2_4422 0.34 -0.1 5,153,905 + Pf6N2E2_4422 0.65 -2.5 5,153,906 - Pf6N2E2_4422 0.65 -1.9 5,154,019 + -1.5 5,154,065 + Pf6N2E2_4423 0.18 -2.7 5,154,066 - Pf6N2E2_4423 0.18 +0.4 5,154,066 - Pf6N2E2_4423 0.18 -1.1 5,154,066 - Pf6N2E2_4423 0.18 +1.3 5,154,205 + Pf6N2E2_4423 0.82 +0.2 5,154,242 + -0.5 5,154,242 + +1.0 5,154,243 - -1.7 5,154,396 + -0.4 5,154,396 + -1.5 5,154,396 + +0.3 5,154,396 + +0.6 5,154,396 + -0.6 5,154,396 + +0.1 5,154,397 - +0.6 5,154,397 - -0.7 5,154,397 - -0.3 5,154,397 - -0.7 5,154,397 - -1.3 5,154,397 - +0.9 5,154,397 - -1.4 5,154,397 - +0.4 5,154,397 - -2.2 5,154,397 - +0.4 5,154,397 - -1.1 5,154,397 - +0.5 5,154,397 - -0.5 5,154,397 - -0.1 5,154,397 - -0.0 5,154,397 - +1.8 5,154,400 + -0.6 5,154,425 + -0.1 5,154,425 + +0.7 5,154,426 - -0.5 5,154,426 - -1.4 5,154,429 + +1.5 5,154,429 + +0.5 5,154,429 + +1.0 5,154,430 - -0.2 5,154,430 - -0.0 5,154,430 - +0.9 5,154,442 + -0.2 5,154,442 + -0.7 5,154,443 - +0.3 5,154,443 - +0.0 5,154,443 - -0.1 5,154,582 + -0.7 5,154,582 + +0.8 5,154,582 + -1.8 5,154,582 + +0.3 5,154,583 - -0.9 5,154,583 - +0.1 5,154,583 - -1.2 5,154,755 + Pf6N2E2_4424 0.51 -0.2 5,154,755 + Pf6N2E2_4424 0.51 +0.9 5,154,755 + Pf6N2E2_4424 0.51 -1.2 5,154,755 + Pf6N2E2_4424 0.51 -0.2 5,154,756 - Pf6N2E2_4424 0.51 +1.1 5,154,756 - Pf6N2E2_4424 0.51 -0.9 5,154,773 + Pf6N2E2_4424 0.55 +0.8 5,155,012 + -0.3 5,155,012 + -1.4 5,155,013 - +0.2 5,155,013 - -0.1 5,155,021 + -0.8 5,155,021 + -0.0
Or see this region's nucleotide sequence