Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4929 and Pf1N1B4_4930 overlap by 4 nucleotides Pf1N1B4_4930 and Pf1N1B4_4931 are separated by 252 nucleotides
Pf1N1B4_4929 - Opine oxidase subunit B, at 5,462,626 to 5,463,795
_4929
Pf1N1B4_4930 - Acetylornithine deacetylase (EC 3.5.1.16), at 5,463,792 to 5,464,949
_4930
Pf1N1B4_4931 - Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20); Succinate-semialdehyde dehydrogenase [NADP+] (EC 1.2.1.79), at 5,465,202 to 5,466,659
_4931
Position (kb) 5463
5464
5465 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5463.325 kb on + strand, within Pf1N1B4_4929 at 5463.353 kb on - strand, within Pf1N1B4_4929 at 5463.353 kb on - strand, within Pf1N1B4_4929 at 5463.379 kb on + strand, within Pf1N1B4_4929 at 5463.379 kb on + strand, within Pf1N1B4_4929 at 5463.460 kb on + strand at 5463.566 kb on - strand, within Pf1N1B4_4929 at 5463.619 kb on + strand, within Pf1N1B4_4929 at 5463.620 kb on - strand, within Pf1N1B4_4929 at 5463.623 kb on - strand, within Pf1N1B4_4929 at 5463.877 kb on - strand at 5463.877 kb on - strand at 5463.877 kb on - strand at 5464.012 kb on + strand, within Pf1N1B4_4930 at 5464.012 kb on + strand, within Pf1N1B4_4930 at 5464.013 kb on - strand, within Pf1N1B4_4930 at 5464.327 kb on - strand, within Pf1N1B4_4930 at 5464.831 kb on + strand at 5464.831 kb on + strand, within Pf1N1B4_4930 at 5464.831 kb on + strand, within Pf1N1B4_4930 at 5464.832 kb on - strand, within Pf1N1B4_4930 at 5465.170 kb on - strand at 5465.250 kb on + strand at 5465.251 kb on - strand at 5465.251 kb on - strand at 5465.251 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 5,463,325 + Pf1N1B4_4929 0.60 -1.6 5,463,353 - Pf1N1B4_4929 0.62 +0.9 5,463,353 - Pf1N1B4_4929 0.62 -0.9 5,463,379 + Pf1N1B4_4929 0.64 -0.6 5,463,379 + Pf1N1B4_4929 0.64 -1.0 5,463,460 + +0.1 5,463,566 - Pf1N1B4_4929 0.80 +0.5 5,463,619 + Pf1N1B4_4929 0.85 -0.5 5,463,620 - Pf1N1B4_4929 0.85 -0.6 5,463,623 - Pf1N1B4_4929 0.85 -0.9 5,463,877 - -1.1 5,463,877 - -0.5 5,463,877 - +2.3 5,464,012 + Pf1N1B4_4930 0.19 +0.6 5,464,012 + Pf1N1B4_4930 0.19 -0.0 5,464,013 - Pf1N1B4_4930 0.19 -1.8 5,464,327 - Pf1N1B4_4930 0.46 -2.3 5,464,831 + -1.5 5,464,831 + Pf1N1B4_4930 0.90 +0.4 5,464,831 + Pf1N1B4_4930 0.90 -1.6 5,464,832 - Pf1N1B4_4930 0.90 +0.1 5,465,170 - -0.1 5,465,250 + -1.5 5,465,251 - -0.1 5,465,251 - +2.1 5,465,251 - -1.1
Or see this region's nucleotide sequence