Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_265 and Pf1N1B4_266 are separated by 284 nucleotides Pf1N1B4_266 and Pf1N1B4_267 overlap by 42 nucleotides Pf1N1B4_267 and Pf1N1B4_268 are separated by 117 nucleotides
Pf1N1B4_265 - DEAD-box ATP-dependent RNA helicase CshA (EC 3.6.4.13), at 324,901 to 326,574
_265
Pf1N1B4_266 - hypothetical protein, at 326,859 to 327,038
_266
Pf1N1B4_267 - Enoyl-CoA hydratase (EC 4.2.1.17), at 326,997 to 327,788
_267
Pf1N1B4_268 - Spermidine synthase (EC 2.5.1.16), at 327,906 to 328,661
_268
Position (kb) 326
327
328 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 326.587 kb on - strand at 326.587 kb on - strand at 326.633 kb on - strand at 326.760 kb on - strand at 326.796 kb on - strand at 326.796 kb on - strand at 326.796 kb on - strand at 326.892 kb on - strand, within Pf1N1B4_266 at 326.892 kb on - strand at 326.892 kb on - strand, within Pf1N1B4_266 at 327.022 kb on + strand at 327.123 kb on + strand, within Pf1N1B4_267 at 327.124 kb on - strand at 327.324 kb on + strand, within Pf1N1B4_267 at 327.324 kb on + strand, within Pf1N1B4_267 at 327.494 kb on + strand, within Pf1N1B4_267 at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.796 kb on - strand at 327.907 kb on + strand at 327.907 kb on + strand at 327.908 kb on - strand at 327.908 kb on - strand at 327.908 kb on - strand at 327.908 kb on - strand at 327.908 kb on - strand at 327.908 kb on - strand at 328.082 kb on - strand, within Pf1N1B4_268 at 328.231 kb on + strand, within Pf1N1B4_268 at 328.232 kb on - strand, within Pf1N1B4_268 at 328.232 kb on - strand, within Pf1N1B4_268 at 328.276 kb on - strand, within Pf1N1B4_268 at 328.303 kb on + strand, within Pf1N1B4_268 at 328.303 kb on + strand, within Pf1N1B4_268 at 328.304 kb on - strand, within Pf1N1B4_268 at 328.304 kb on - strand, within Pf1N1B4_268 at 328.304 kb on - strand, within Pf1N1B4_268 at 328.304 kb on - strand, within Pf1N1B4_268 at 328.333 kb on + strand, within Pf1N1B4_268 at 328.334 kb on - strand, within Pf1N1B4_268 at 328.502 kb on - strand, within Pf1N1B4_268 at 328.507 kb on + strand, within Pf1N1B4_268 at 328.507 kb on + strand, within Pf1N1B4_268 at 328.508 kb on - strand, within Pf1N1B4_268 at 328.623 kb on + strand at 328.623 kb on + strand at 328.624 kb on - strand at 328.696 kb on + strand at 328.696 kb on + strand at 328.696 kb on + strand at 328.696 kb on + strand at 328.696 kb on + strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.699 kb on + strand at 328.699 kb on + strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.765 kb on + strand at 328.765 kb on + strand at 328.765 kb on + strand at 328.766 kb on - strand at 328.766 kb on - strand at 328.775 kb on + strand at 328.780 kb on + strand at 328.781 kb on - strand at 328.781 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 326,587 - -0.1 326,587 - +2.6 326,633 - -1.4 326,760 - +0.3 326,796 - +0.7 326,796 - -2.7 326,796 - +0.7 326,892 - Pf1N1B4_266 0.18 +0.1 326,892 - +1.4 326,892 - Pf1N1B4_266 0.18 -0.4 327,022 + -1.2 327,123 + Pf1N1B4_267 0.16 -2.5 327,124 - +0.2 327,324 + Pf1N1B4_267 0.41 -1.4 327,324 + Pf1N1B4_267 0.41 -0.5 327,494 + Pf1N1B4_267 0.63 -0.3 327,795 + -0.5 327,795 + -0.2 327,795 + -1.0 327,795 + -0.0 327,795 + +0.1 327,795 + -1.3 327,795 + -0.7 327,795 + +0.2 327,795 + +0.4 327,796 - +0.4 327,907 + +0.1 327,907 + +0.7 327,908 - +0.3 327,908 - -0.2 327,908 - -0.9 327,908 - +0.3 327,908 - -1.1 327,908 - +0.2 328,082 - Pf1N1B4_268 0.23 -2.2 328,231 + Pf1N1B4_268 0.43 +0.1 328,232 - Pf1N1B4_268 0.43 +0.8 328,232 - Pf1N1B4_268 0.43 -0.6 328,276 - Pf1N1B4_268 0.49 -1.6 328,303 + Pf1N1B4_268 0.53 +0.8 328,303 + Pf1N1B4_268 0.53 +0.3 328,304 - Pf1N1B4_268 0.53 -1.2 328,304 - Pf1N1B4_268 0.53 +0.7 328,304 - Pf1N1B4_268 0.53 +0.7 328,304 - Pf1N1B4_268 0.53 -1.4 328,333 + Pf1N1B4_268 0.56 -0.5 328,334 - Pf1N1B4_268 0.57 +0.8 328,502 - Pf1N1B4_268 0.79 -0.6 328,507 + Pf1N1B4_268 0.79 -0.7 328,507 + Pf1N1B4_268 0.79 -1.2 328,508 - Pf1N1B4_268 0.80 -1.4 328,623 + +0.4 328,623 + +0.4 328,624 - -1.5 328,696 + -0.5 328,696 + -0.4 328,696 + -0.6 328,696 + -0.6 328,696 + +0.0 328,697 - +0.0 328,697 - +0.8 328,697 - +2.1 328,697 - +1.2 328,697 - -0.2 328,697 - +0.5 328,697 - +0.1 328,697 - +0.9 328,697 - -0.2 328,699 + +1.8 328,699 + +0.1 328,700 - -0.1 328,700 - -0.7 328,700 - +0.2 328,700 - +0.9 328,700 - -0.4 328,700 - -2.8 328,700 - +0.2 328,700 - +0.5 328,765 + -0.4 328,765 + +0.5 328,765 + -0.5 328,766 - +0.8 328,766 - +1.1 328,775 + +0.2 328,780 + -1.0 328,781 - -0.9 328,781 - +0.0
Or see this region's nucleotide sequence