Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00300 and RR42_RS00305 are separated by 87 nucleotides RR42_RS00305 and RR42_RS00310 are separated by 137 nucleotides RR42_RS00310 and RR42_RS00315 are separated by 43 nucleotides
RR42_RS00300: RR42_RS00300 - kinase, at 86,073 to 86,921
_RS00300
RR42_RS00305: RR42_RS00305 - hypothetical protein, at 87,009 to 88,082
_RS00305
RR42_RS00310: RR42_RS00310 - TetR family transcriptional regulator, at 88,220 to 88,888
_RS00310
RR42_RS00315: RR42_RS00315 - iron-binding protein, at 88,932 to 89,579
_RS00315
Position (kb) 87
88
89 Strain fitness (log2 ratio) -2
-1
0
1
2 at 86.166 kb on + strand, within RR42_RS00300 at 86.166 kb on + strand, within RR42_RS00300 at 86.167 kb on - strand, within RR42_RS00300 at 86.167 kb on - strand, within RR42_RS00300 at 86.167 kb on - strand, within RR42_RS00300 at 86.746 kb on - strand, within RR42_RS00300 at 86.769 kb on + strand, within RR42_RS00300 at 86.769 kb on + strand, within RR42_RS00300 at 87.022 kb on + strand at 87.023 kb on - strand at 87.023 kb on - strand at 87.023 kb on - strand at 87.023 kb on - strand at 87.143 kb on + strand, within RR42_RS00305 at 87.144 kb on - strand, within RR42_RS00305 at 87.232 kb on + strand, within RR42_RS00305 at 87.232 kb on + strand, within RR42_RS00305 at 87.310 kb on + strand, within RR42_RS00305 at 87.311 kb on - strand, within RR42_RS00305 at 87.379 kb on + strand, within RR42_RS00305 at 87.379 kb on + strand, within RR42_RS00305 at 87.380 kb on - strand, within RR42_RS00305 at 87.380 kb on - strand, within RR42_RS00305 at 87.380 kb on - strand, within RR42_RS00305 at 87.380 kb on - strand, within RR42_RS00305 at 87.380 kb on - strand, within RR42_RS00305 at 87.422 kb on - strand, within RR42_RS00305 at 87.440 kb on - strand, within RR42_RS00305 at 87.482 kb on + strand, within RR42_RS00305 at 87.482 kb on + strand, within RR42_RS00305 at 87.483 kb on - strand, within RR42_RS00305 at 87.644 kb on - strand, within RR42_RS00305 at 87.703 kb on + strand, within RR42_RS00305 at 87.703 kb on + strand, within RR42_RS00305 at 87.703 kb on + strand, within RR42_RS00305 at 87.704 kb on - strand, within RR42_RS00305 at 87.712 kb on + strand, within RR42_RS00305 at 87.751 kb on + strand, within RR42_RS00305 at 87.752 kb on - strand, within RR42_RS00305 at 87.752 kb on - strand, within RR42_RS00305 at 87.752 kb on - strand, within RR42_RS00305 at 87.791 kb on + strand, within RR42_RS00305 at 87.792 kb on - strand, within RR42_RS00305 at 87.811 kb on + strand, within RR42_RS00305 at 87.818 kb on - strand, within RR42_RS00305 at 87.818 kb on - strand, within RR42_RS00305 at 87.888 kb on - strand, within RR42_RS00305 at 87.928 kb on + strand, within RR42_RS00305 at 87.955 kb on + strand, within RR42_RS00305 at 87.956 kb on - strand, within RR42_RS00305 at 88.060 kb on - strand at 88.060 kb on - strand at 88.232 kb on + strand at 88.233 kb on - strand at 88.355 kb on + strand, within RR42_RS00310 at 88.356 kb on - strand, within RR42_RS00310 at 88.356 kb on - strand, within RR42_RS00310 at 88.457 kb on + strand, within RR42_RS00310 at 88.457 kb on + strand, within RR42_RS00310 at 88.457 kb on + strand, within RR42_RS00310 at 88.491 kb on - strand, within RR42_RS00310 at 88.491 kb on - strand, within RR42_RS00310 at 88.491 kb on - strand, within RR42_RS00310 at 88.704 kb on - strand, within RR42_RS00310 at 88.704 kb on - strand, within RR42_RS00310 at 88.704 kb on - strand, within RR42_RS00310 at 88.704 kb on - strand, within RR42_RS00310 at 88.916 kb on - strand at 88.925 kb on - strand at 88.933 kb on + strand at 88.933 kb on + strand at 88.933 kb on + strand at 88.934 kb on - strand at 88.934 kb on - strand at 88.935 kb on + strand at 88.935 kb on + strand at 88.935 kb on + strand at 88.936 kb on - strand at 88.936 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 86,166 + RR42_RS00300 0.11 -1.2 86,166 + RR42_RS00300 0.11 +0.0 86,167 - RR42_RS00300 0.11 -0.2 86,167 - RR42_RS00300 0.11 +0.3 86,167 - RR42_RS00300 0.11 +1.6 86,746 - RR42_RS00300 0.79 +2.0 86,769 + RR42_RS00300 0.82 +0.2 86,769 + RR42_RS00300 0.82 +0.1 87,022 + -0.4 87,023 - -0.3 87,023 - +0.4 87,023 - -1.2 87,023 - +1.3 87,143 + RR42_RS00305 0.12 +1.0 87,144 - RR42_RS00305 0.13 +0.4 87,232 + RR42_RS00305 0.21 -1.1 87,232 + RR42_RS00305 0.21 -1.4 87,310 + RR42_RS00305 0.28 +1.4 87,311 - RR42_RS00305 0.28 +0.6 87,379 + RR42_RS00305 0.34 -0.9 87,379 + RR42_RS00305 0.34 +0.6 87,380 - RR42_RS00305 0.35 +0.4 87,380 - RR42_RS00305 0.35 -0.0 87,380 - RR42_RS00305 0.35 -0.7 87,380 - RR42_RS00305 0.35 -0.4 87,380 - RR42_RS00305 0.35 -1.1 87,422 - RR42_RS00305 0.38 -0.0 87,440 - RR42_RS00305 0.40 +0.3 87,482 + RR42_RS00305 0.44 -0.9 87,482 + RR42_RS00305 0.44 -0.4 87,483 - RR42_RS00305 0.44 -0.6 87,644 - RR42_RS00305 0.59 +0.3 87,703 + RR42_RS00305 0.65 -1.0 87,703 + RR42_RS00305 0.65 -0.7 87,703 + RR42_RS00305 0.65 +1.0 87,704 - RR42_RS00305 0.65 +0.3 87,712 + RR42_RS00305 0.65 -0.1 87,751 + RR42_RS00305 0.69 +0.3 87,752 - RR42_RS00305 0.69 -1.0 87,752 - RR42_RS00305 0.69 +0.2 87,752 - RR42_RS00305 0.69 -0.3 87,791 + RR42_RS00305 0.73 -0.3 87,792 - RR42_RS00305 0.73 +0.6 87,811 + RR42_RS00305 0.75 +0.2 87,818 - RR42_RS00305 0.75 -0.3 87,818 - RR42_RS00305 0.75 +0.3 87,888 - RR42_RS00305 0.82 -1.3 87,928 + RR42_RS00305 0.86 -0.6 87,955 + RR42_RS00305 0.88 +0.0 87,956 - RR42_RS00305 0.88 +0.1 88,060 - +1.2 88,060 - +0.1 88,232 + -0.1 88,233 - -1.8 88,355 + RR42_RS00310 0.20 -0.5 88,356 - RR42_RS00310 0.20 -0.1 88,356 - RR42_RS00310 0.20 +0.1 88,457 + RR42_RS00310 0.35 -0.4 88,457 + RR42_RS00310 0.35 -0.5 88,457 + RR42_RS00310 0.35 +0.2 88,491 - RR42_RS00310 0.41 +0.3 88,491 - RR42_RS00310 0.41 +0.3 88,491 - RR42_RS00310 0.41 +0.2 88,704 - RR42_RS00310 0.72 +0.4 88,704 - RR42_RS00310 0.72 +0.1 88,704 - RR42_RS00310 0.72 -1.0 88,704 - RR42_RS00310 0.72 -0.1 88,916 - +0.4 88,925 - -0.2 88,933 + -0.9 88,933 + -0.0 88,933 + -0.4 88,934 - +1.0 88,934 - +2.6 88,935 + -0.1 88,935 + -0.0 88,935 + +0.4 88,936 - +1.0 88,936 - -0.1
Or see this region's nucleotide sequence