Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20660 and RR42_RS20665 are separated by 163 nucleotides RR42_RS20665 and RR42_RS20670 are separated by 24 nucleotides RR42_RS20670 and RR42_RS20675 are separated by 155 nucleotides RR42_RS20675 and RR42_RS20680 are separated by 83 nucleotides
RR42_RS20660: RR42_RS20660 - GNAT family acetyltransferase, at 4,487,316 to 4,487,774
_RS20660
RR42_RS20665: RR42_RS20665 - MBL fold metallo-hydrolase, at 4,487,938 to 4,488,669
_RS20665
RR42_RS20670: RR42_RS20670 - glutathione S-transferase, at 4,488,694 to 4,489,302
_RS20670
RR42_RS20675: RR42_RS20675 - AsnC family transcriptional regulator, at 4,489,458 to 4,489,916
_RS20675
RR42_RS20680: RR42_RS20680 - membrane protein, at 4,490,000 to 4,490,851
_RS20680
Position (kb) 4488
4489
4490 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4487.719 kb on - strand, within RR42_RS20660 at 4487.719 kb on - strand, within RR42_RS20660 at 4487.996 kb on - strand at 4488.056 kb on - strand, within RR42_RS20665 at 4488.165 kb on + strand, within RR42_RS20665 at 4488.166 kb on - strand, within RR42_RS20665 at 4488.523 kb on + strand, within RR42_RS20665 at 4488.523 kb on + strand, within RR42_RS20665 at 4488.523 kb on + strand, within RR42_RS20665 at 4488.523 kb on + strand, within RR42_RS20665 at 4488.523 kb on + strand, within RR42_RS20665 at 4488.668 kb on - strand at 4488.668 kb on - strand at 4488.764 kb on + strand, within RR42_RS20670 at 4488.765 kb on - strand, within RR42_RS20670 at 4488.829 kb on + strand, within RR42_RS20670 at 4488.830 kb on - strand, within RR42_RS20670 at 4489.098 kb on - strand, within RR42_RS20670 at 4489.271 kb on + strand at 4489.272 kb on - strand at 4489.302 kb on + strand at 4489.303 kb on - strand at 4489.356 kb on + strand at 4489.356 kb on + strand at 4489.668 kb on - strand, within RR42_RS20675 at 4489.668 kb on - strand, within RR42_RS20675 at 4489.686 kb on + strand, within RR42_RS20675 at 4489.686 kb on + strand, within RR42_RS20675 at 4489.686 kb on + strand, within RR42_RS20675 at 4489.909 kb on + strand at 4489.909 kb on + strand at 4489.919 kb on + strand at 4489.919 kb on + strand at 4489.919 kb on + strand at 4489.920 kb on - strand at 4490.008 kb on + strand at 4490.127 kb on + strand, within RR42_RS20680 at 4490.127 kb on + strand, within RR42_RS20680 at 4490.128 kb on - strand, within RR42_RS20680
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 4,487,719 - RR42_RS20660 0.88 -0.9 4,487,719 - RR42_RS20660 0.88 -0.6 4,487,996 - +0.3 4,488,056 - RR42_RS20665 0.16 -1.3 4,488,165 + RR42_RS20665 0.31 -0.6 4,488,166 - RR42_RS20665 0.31 +2.0 4,488,523 + RR42_RS20665 0.80 -0.0 4,488,523 + RR42_RS20665 0.80 -1.1 4,488,523 + RR42_RS20665 0.80 +0.9 4,488,523 + RR42_RS20665 0.80 +0.4 4,488,523 + RR42_RS20665 0.80 +0.5 4,488,668 - +0.5 4,488,668 - -0.3 4,488,764 + RR42_RS20670 0.11 +0.3 4,488,765 - RR42_RS20670 0.12 -0.5 4,488,829 + RR42_RS20670 0.22 -0.4 4,488,830 - RR42_RS20670 0.22 +0.3 4,489,098 - RR42_RS20670 0.66 -1.2 4,489,271 + +1.9 4,489,272 - +0.4 4,489,302 + -0.7 4,489,303 - +0.9 4,489,356 + +0.1 4,489,356 + +0.1 4,489,668 - RR42_RS20675 0.46 +0.3 4,489,668 - RR42_RS20675 0.46 -0.8 4,489,686 + RR42_RS20675 0.50 +0.3 4,489,686 + RR42_RS20675 0.50 -0.8 4,489,686 + RR42_RS20675 0.50 -0.3 4,489,909 + +0.0 4,489,909 + -0.2 4,489,919 + -0.0 4,489,919 + -0.4 4,489,919 + -0.3 4,489,920 - -2.2 4,490,008 + +1.1 4,490,127 + RR42_RS20680 0.15 +1.2 4,490,127 + RR42_RS20680 0.15 +0.6 4,490,128 - RR42_RS20680 0.15 +1.2
Or see this region's nucleotide sequence