Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35710 and RR42_RS35715 are separated by 56 nucleotides RR42_RS35715 and RR42_RS35720 are separated by 14 nucleotides RR42_RS35720 and RR42_RS35725 are separated by 7 nucleotides RR42_RS35725 and RR42_RS35730 are separated by 222 nucleotides
RR42_RS35710: RR42_RS35710 - flagellar hook protein FlgE, at 3,462,244 to 3,463,485
_RS35710
RR42_RS35715: RR42_RS35715 - flagellar basal body rod modification protein FlgD, at 3,463,542 to 3,464,213
_RS35715
RR42_RS35720: RR42_RS35720 - flagellar basal body rod protein FlgC, at 3,464,228 to 3,464,635
_RS35720
RR42_RS35725: RR42_RS35725 - flagellar biosynthesis protein FlgB, at 3,464,643 to 3,465,053
_RS35725
RR42_RS35730: RR42_RS35730 - flagellar basal body P-ring biosynthesis protein FlgA, at 3,465,276 to 3,465,995
_RS35730
Position (kb)
3464
3465 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3463.496 kb on + strand at 3463.501 kb on - strand at 3463.544 kb on - strand at 3463.702 kb on + strand, within RR42_RS35715 at 3463.977 kb on + strand, within RR42_RS35715 at 3464.298 kb on + strand, within RR42_RS35720 at 3464.298 kb on + strand, within RR42_RS35720 at 3464.679 kb on - strand at 3464.942 kb on - strand, within RR42_RS35725 at 3464.951 kb on + strand, within RR42_RS35725 at 3464.951 kb on + strand, within RR42_RS35725 at 3464.951 kb on + strand, within RR42_RS35725 at 3465.197 kb on + strand at 3465.197 kb on + strand at 3465.203 kb on - strand at 3465.203 kb on - strand at 3465.203 kb on - strand at 3465.203 kb on - strand at 3465.271 kb on + strand at 3465.271 kb on + strand at 3465.271 kb on + strand at 3465.272 kb on - strand at 3465.272 kb on - strand at 3465.272 kb on - strand at 3465.272 kb on - strand at 3465.272 kb on - strand at 3465.272 kb on - strand at 3465.464 kb on - strand, within RR42_RS35730 at 3465.591 kb on + strand, within RR42_RS35730 at 3465.592 kb on - strand, within RR42_RS35730 at 3465.624 kb on + strand, within RR42_RS35730 at 3465.625 kb on - strand, within RR42_RS35730 at 3465.627 kb on + strand, within RR42_RS35730 at 3465.627 kb on + strand, within RR42_RS35730 at 3465.627 kb on + strand, within RR42_RS35730 at 3465.627 kb on + strand, within RR42_RS35730 at 3465.628 kb on - strand, within RR42_RS35730 at 3465.628 kb on - strand, within RR42_RS35730 at 3465.628 kb on - strand, within RR42_RS35730
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,463,496 + -1.3 3,463,501 - +0.4 3,463,544 - -1.0 3,463,702 + RR42_RS35715 0.24 -0.1 3,463,977 + RR42_RS35715 0.65 -0.4 3,464,298 + RR42_RS35720 0.17 +0.3 3,464,298 + RR42_RS35720 0.17 -1.5 3,464,679 - +0.6 3,464,942 - RR42_RS35725 0.73 +0.1 3,464,951 + RR42_RS35725 0.75 +0.6 3,464,951 + RR42_RS35725 0.75 +1.6 3,464,951 + RR42_RS35725 0.75 +0.2 3,465,197 + +0.7 3,465,197 + -0.4 3,465,203 - -0.2 3,465,203 - +0.6 3,465,203 - -0.1 3,465,203 - -1.9 3,465,271 + -2.1 3,465,271 + +0.2 3,465,271 + +0.7 3,465,272 - -0.5 3,465,272 - +0.3 3,465,272 - +0.6 3,465,272 - -1.3 3,465,272 - +0.7 3,465,272 - +0.1 3,465,464 - RR42_RS35730 0.26 -1.1 3,465,591 + RR42_RS35730 0.44 -0.1 3,465,592 - RR42_RS35730 0.44 +0.9 3,465,624 + RR42_RS35730 0.48 +0.1 3,465,625 - RR42_RS35730 0.48 +3.2 3,465,627 + RR42_RS35730 0.49 -0.6 3,465,627 + RR42_RS35730 0.49 +0.3 3,465,627 + RR42_RS35730 0.49 -1.0 3,465,627 + RR42_RS35730 0.49 -0.9 3,465,628 - RR42_RS35730 0.49 +2.2 3,465,628 - RR42_RS35730 0.49 +0.5 3,465,628 - RR42_RS35730 0.49 +0.5
Or see this region's nucleotide sequence