Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01375 and RR42_RS01380 are separated by 129 nucleotides RR42_RS01380 and RR42_RS01385 are separated by 62 nucleotides RR42_RS01385 and RR42_RS01390 are separated by 22 nucleotides RR42_RS01390 and RR42_RS01395 are separated by 31 nucleotides
RR42_RS01375: RR42_RS01375 - S-adenosyl-L-homocysteine hydrolase, at 311,482 to 312,915
_RS01375
RR42_RS01380: RR42_RS01380 - membrane protein, at 313,045 to 313,398
_RS01380
RR42_RS01385: RR42_RS01385 - 5,10-methylenetetrahydrofolate reductase, at 313,461 to 314,291
_RS01385
RR42_RS01390: RR42_RS01390 - competence protein, at 314,314 to 314,676
_RS01390
RR42_RS01395: RR42_RS01395 - twitching motility protein PilT, at 314,708 to 315,475
_RS01395
Position (kb) 313
314
315 Strain fitness (log2 ratio) -2
-1
0
1 at 313.173 kb on + strand, within RR42_RS01380 at 313.173 kb on + strand, within RR42_RS01380 at 313.174 kb on - strand, within RR42_RS01380 at 313.174 kb on - strand, within RR42_RS01380 at 313.174 kb on - strand, within RR42_RS01380 at 313.340 kb on - strand, within RR42_RS01380 at 313.717 kb on - strand, within RR42_RS01385 at 313.875 kb on + strand, within RR42_RS01385 at 313.962 kb on + strand, within RR42_RS01385 at 314.235 kb on + strand at 314.237 kb on + strand at 314.237 kb on + strand at 314.289 kb on + strand at 314.290 kb on - strand at 314.428 kb on + strand, within RR42_RS01390 at 314.428 kb on + strand, within RR42_RS01390 at 314.431 kb on + strand, within RR42_RS01390 at 314.431 kb on + strand, within RR42_RS01390 at 314.432 kb on - strand, within RR42_RS01390 at 314.432 kb on - strand, within RR42_RS01390 at 314.474 kb on - strand, within RR42_RS01390 at 314.572 kb on + strand, within RR42_RS01390 at 314.573 kb on - strand, within RR42_RS01390 at 314.697 kb on + strand at 314.697 kb on + strand at 314.751 kb on - strand at 314.945 kb on + strand, within RR42_RS01395 at 315.083 kb on + strand, within RR42_RS01395 at 315.084 kb on - strand, within RR42_RS01395 at 315.095 kb on + strand, within RR42_RS01395 at 315.096 kb on - strand, within RR42_RS01395 at 315.096 kb on - strand, within RR42_RS01395 at 315.197 kb on + strand, within RR42_RS01395 at 315.198 kb on - strand, within RR42_RS01395 at 315.198 kb on - strand, within RR42_RS01395
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 313,173 + RR42_RS01380 0.36 +0.2 313,173 + RR42_RS01380 0.36 -2.0 313,174 - RR42_RS01380 0.36 -0.9 313,174 - RR42_RS01380 0.36 -0.7 313,174 - RR42_RS01380 0.36 -0.8 313,340 - RR42_RS01380 0.83 +1.0 313,717 - RR42_RS01385 0.31 -0.6 313,875 + RR42_RS01385 0.50 -0.4 313,962 + RR42_RS01385 0.60 -2.3 314,235 + -1.3 314,237 + -0.5 314,237 + -0.5 314,289 + -0.9 314,290 - +0.0 314,428 + RR42_RS01390 0.31 -1.0 314,428 + RR42_RS01390 0.31 +1.2 314,431 + RR42_RS01390 0.32 -2.3 314,431 + RR42_RS01390 0.32 +0.6 314,432 - RR42_RS01390 0.33 +0.6 314,432 - RR42_RS01390 0.33 +0.4 314,474 - RR42_RS01390 0.44 -1.5 314,572 + RR42_RS01390 0.71 -1.7 314,573 - RR42_RS01390 0.71 -1.0 314,697 + +0.2 314,697 + -1.5 314,751 - -0.1 314,945 + RR42_RS01395 0.31 -0.2 315,083 + RR42_RS01395 0.49 -0.9 315,084 - RR42_RS01395 0.49 -2.0 315,095 + RR42_RS01395 0.50 -0.8 315,096 - RR42_RS01395 0.51 +0.3 315,096 - RR42_RS01395 0.51 +0.2 315,197 + RR42_RS01395 0.64 -0.4 315,198 - RR42_RS01395 0.64 -0.2 315,198 - RR42_RS01395 0.64 -0.6
Or see this region's nucleotide sequence