Experiment: Adenine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4002 and Pf1N1B4_4003 are separated by 126 nucleotides Pf1N1B4_4003 and Pf1N1B4_4004 overlap by 4 nucleotides Pf1N1B4_4004 and Pf1N1B4_4005 are separated by 262 nucleotides
Pf1N1B4_4002 - Inner membrane component of tripartite multidrug resistance system, at 4,450,875 to 4,452,404
_4002
Pf1N1B4_4003 - UDP-2,3-diacylglucosamine diphosphatase (EC 3.6.1.54), at 4,452,531 to 4,453,283
_4003
Pf1N1B4_4004 - Peptidyl-prolyl cis-trans isomerase PpiB (EC 5.2.1.8), at 4,453,280 to 4,453,783
_4004
Pf1N1B4_4005 - Glutaminyl-tRNA synthetase (EC 6.1.1.18), at 4,454,046 to 4,455,743
_4005
Position (kb)
4453
4454 Strain fitness (log2 ratio)
-1
0
1 at 4452.300 kb on + strand at 4452.301 kb on - strand at 4453.326 kb on - strand at 4453.410 kb on - strand, within Pf1N1B4_4004 at 4453.417 kb on - strand, within Pf1N1B4_4004 at 4453.534 kb on - strand, within Pf1N1B4_4004 at 4453.800 kb on - strand at 4453.825 kb on - strand at 4453.870 kb on + strand at 4453.918 kb on + strand at 4453.919 kb on - strand at 4453.974 kb on + strand at 4454.030 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine (N) remove 4,452,300 + +0.2 4,452,301 - +1.2 4,453,326 - +0.7 4,453,410 - Pf1N1B4_4004 0.26 +0.1 4,453,417 - Pf1N1B4_4004 0.27 +1.7 4,453,534 - Pf1N1B4_4004 0.50 +0.7 4,453,800 - +0.7 4,453,825 - -0.3 4,453,870 + -0.3 4,453,918 + -0.3 4,453,919 - -0.9 4,453,974 + -0.3 4,454,030 + -0.8
Or see this region's nucleotide sequence