Experiment: D-Sorbitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05840 and PGA1_c05850 are separated by 361 nucleotides PGA1_c05850 and PGA1_c05860 overlap by 4 nucleotides PGA1_c05860 and PGA1_c05870 are separated by 99 nucleotides
PGA1_c05840: PGA1_c05840 - Uncharacterized conserved small protein, at 598,729 to 598,947
_c05840
PGA1_c05850: PGA1_c05850 - crossover junction endodeoxyribonuclease RuvC, at 599,309 to 599,806
_c05850
PGA1_c05860: PGA1_c05860 - holliday junction ATP-dependent DNA helicase RuvA, at 599,803 to 600,492
_c05860
PGA1_c05870: PGA1_c05870 - holliday junction ATP-dependent DNA helicase RuvB, at 600,592 to 601,641
_c05870
Position (kb) 599
600
601 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 598.943 kb on + strand at 599.009 kb on - strand at 599.103 kb on + strand at 599.111 kb on - strand at 599.144 kb on - strand at 599.291 kb on + strand at 599.296 kb on + strand at 599.304 kb on - strand at 599.309 kb on - strand at 599.309 kb on - strand at 599.357 kb on - strand at 599.562 kb on + strand, within PGA1_c05850 at 599.562 kb on - strand, within PGA1_c05850 at 599.577 kb on + strand, within PGA1_c05850 at 599.759 kb on - strand at 599.784 kb on + strand at 599.812 kb on + strand at 599.894 kb on - strand, within PGA1_c05860 at 599.921 kb on + strand, within PGA1_c05860 at 599.941 kb on + strand, within PGA1_c05860 at 599.976 kb on - strand, within PGA1_c05860 at 600.197 kb on - strand, within PGA1_c05860 at 600.203 kb on - strand, within PGA1_c05860 at 600.461 kb on - strand at 600.515 kb on - strand at 600.518 kb on + strand at 600.556 kb on + strand at 600.726 kb on - strand, within PGA1_c05870 at 600.881 kb on - strand, within PGA1_c05870 at 600.891 kb on - strand, within PGA1_c05870 at 601.124 kb on - strand, within PGA1_c05870 at 601.351 kb on - strand, within PGA1_c05870 at 601.378 kb on + strand, within PGA1_c05870 at 601.461 kb on - strand, within PGA1_c05870
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol (C) remove 598,943 + +0.1 599,009 - +0.5 599,103 + -2.3 599,111 - -2.4 599,144 - -1.5 599,291 + +0.5 599,296 + -0.6 599,304 - +0.2 599,309 - +0.6 599,309 - +0.5 599,357 - -2.1 599,562 + PGA1_c05850 0.51 +0.6 599,562 - PGA1_c05850 0.51 +0.5 599,577 + PGA1_c05850 0.54 -1.7 599,759 - -0.1 599,784 + -2.3 599,812 + -3.0 599,894 - PGA1_c05860 0.13 -1.4 599,921 + PGA1_c05860 0.17 -1.0 599,941 + PGA1_c05860 0.20 -2.1 599,976 - PGA1_c05860 0.25 -2.1 600,197 - PGA1_c05860 0.57 -1.0 600,203 - PGA1_c05860 0.58 -0.5 600,461 - +0.6 600,515 - -0.0 600,518 + +0.7 600,556 + +0.1 600,726 - PGA1_c05870 0.13 -2.1 600,881 - PGA1_c05870 0.28 -2.1 600,891 - PGA1_c05870 0.28 -0.9 601,124 - PGA1_c05870 0.51 -2.8 601,351 - PGA1_c05870 0.72 -0.9 601,378 + PGA1_c05870 0.75 -0.1 601,461 - PGA1_c05870 0.83 -2.8
Or see this region's nucleotide sequence