Strain Fitness in Shewanella oneidensis MR-1 around SO3235

Experiment: L-Methionine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliS and SO3234 are separated by 6 nucleotidesSO3234 and fliD are separated by 28 nucleotidesfliD and flaG are separated by 42 nucleotidesflaG and SO3237 are separated by 156 nucleotides SO3233: fliS - flagellar protein FliS (NCBI ptt file), at 3,373,086 to 3,373,496 fliS SO3234: SO3234 - hypothetical protein (NCBI ptt file), at 3,373,503 to 3,373,823 SO3234 SO3235: fliD - flagellar hook-associated protein FliD (NCBI ptt file), at 3,373,852 to 3,375,222 fliD SO3236: flaG - flagellin FlaG (NCBI ptt file), at 3,375,265 to 3,375,624 flaG SO3237: SO3237 - flagellin (NCBI ptt file), at 3,375,781 to 3,376,599 SO3237 Position (kb) 3373 3374 3375 3376Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6at 3372.884 kb on - strandat 3372.901 kb on - strandat 3372.917 kb on + strandat 3373.116 kb on + strandat 3373.116 kb on + strandat 3373.116 kb on + strandat 3373.136 kb on + strand, within fliSat 3373.145 kb on - strand, within fliSat 3373.156 kb on - strand, within fliSat 3373.361 kb on + strand, within fliSat 3373.370 kb on + strand, within fliSat 3373.377 kb on + strand, within fliSat 3373.377 kb on + strand, within fliSat 3373.385 kb on - strand, within fliSat 3373.385 kb on - strand, within fliSat 3373.385 kb on - strand, within fliSat 3373.389 kb on + strand, within fliSat 3373.389 kb on + strand, within fliSat 3373.397 kb on - strand, within fliSat 3373.400 kb on - strand, within fliSat 3373.426 kb on - strand, within fliSat 3373.500 kb on + strandat 3373.500 kb on - strandat 3373.539 kb on - strand, within SO3234at 3373.624 kb on + strand, within SO3234at 3373.684 kb on - strand, within SO3234at 3373.711 kb on - strand, within SO3234at 3373.711 kb on - strand, within SO3234at 3373.733 kb on + strand, within SO3234at 3373.749 kb on + strand, within SO3234at 3373.775 kb on + strand, within SO3234at 3373.775 kb on + strand, within SO3234at 3373.783 kb on - strand, within SO3234at 3373.801 kb on - strandat 3374.020 kb on + strand, within fliDat 3374.028 kb on - strand, within fliDat 3374.036 kb on - strand, within fliDat 3374.178 kb on + strand, within fliDat 3374.323 kb on + strand, within fliDat 3374.331 kb on - strand, within fliDat 3374.371 kb on - strand, within fliDat 3374.468 kb on + strand, within fliDat 3374.642 kb on - strand, within fliDat 3374.642 kb on - strand, within fliDat 3374.698 kb on + strand, within fliDat 3374.740 kb on + strand, within fliDat 3374.977 kb on + strand, within fliDat 3374.982 kb on + strand, within fliDat 3375.121 kb on + strandat 3375.184 kb on + strandat 3375.196 kb on + strandat 3375.262 kb on + strandat 3375.629 kb on - strandat 3375.715 kb on + strandat 3375.966 kb on - strand, within SO3237at 3375.981 kb on - strand, within SO3237at 3375.983 kb on + strand, within SO3237at 3376.122 kb on + strand, within SO3237at 3376.196 kb on + strand, within SO3237

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Methionine (N)
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3,372,884 - +3.7
3,372,901 - +0.3
3,372,917 + +1.9
3,373,116 + +1.8
3,373,116 + +2.3
3,373,116 + +2.2
3,373,136 + fliS SO3233 0.12 +4.0
3,373,145 - fliS SO3233 0.14 +4.6
3,373,156 - fliS SO3233 0.17 +3.2
3,373,361 + fliS SO3233 0.67 +3.8
3,373,370 + fliS SO3233 0.69 +5.7
3,373,377 + fliS SO3233 0.71 +3.8
3,373,377 + fliS SO3233 0.71 +4.4
3,373,385 - fliS SO3233 0.73 +3.2
3,373,385 - fliS SO3233 0.73 +6.1
3,373,385 - fliS SO3233 0.73 +3.7
3,373,389 + fliS SO3233 0.74 +4.9
3,373,389 + fliS SO3233 0.74 +4.3
3,373,397 - fliS SO3233 0.76 +4.6
3,373,400 - fliS SO3233 0.76 +2.9
3,373,426 - fliS SO3233 0.83 +4.7
3,373,500 + +3.0
3,373,500 - -1.0
3,373,539 - SO3234 0.11 -1.6
3,373,624 + SO3234 0.38 +2.3
3,373,684 - SO3234 0.56 +1.7
3,373,711 - SO3234 0.65 +2.5
3,373,711 - SO3234 0.65 +1.4
3,373,733 + SO3234 0.72 +3.7
3,373,749 + SO3234 0.77 +2.5
3,373,775 + SO3234 0.85 +3.7
3,373,775 + SO3234 0.85 +3.5
3,373,783 - SO3234 0.87 +2.2
3,373,801 - +2.5
3,374,020 + fliD SO3235 0.12 +4.2
3,374,028 - fliD SO3235 0.13 +2.6
3,374,036 - fliD SO3235 0.13 +3.2
3,374,178 + fliD SO3235 0.24 +2.1
3,374,323 + fliD SO3235 0.34 +1.7
3,374,331 - fliD SO3235 0.35 +2.1
3,374,371 - fliD SO3235 0.38 +3.0
3,374,468 + fliD SO3235 0.45 +1.1
3,374,642 - fliD SO3235 0.58 +3.2
3,374,642 - fliD SO3235 0.58 +3.1
3,374,698 + fliD SO3235 0.62 +2.2
3,374,740 + fliD SO3235 0.65 +2.5
3,374,977 + fliD SO3235 0.82 +2.0
3,374,982 + fliD SO3235 0.82 +3.4
3,375,121 + +3.8
3,375,184 + +5.0
3,375,196 + +3.3
3,375,262 + +2.6
3,375,629 - +1.8
3,375,715 + +2.6
3,375,966 - SO3237 0.23 +2.0
3,375,981 - SO3237 0.24 +3.8
3,375,983 + SO3237 0.25 +2.2
3,376,122 + SO3237 0.42 +4.3
3,376,196 + SO3237 0.51 +2.0

Or see this region's nucleotide sequence