Experiment: Outgrowth from conjugation with pRH23 in LB+Gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfcI and hisP are separated by 196 nucleotides hisP and hisM are separated by 7 nucleotides hisM and hisQ overlap by 4 nucleotides hisQ and hisJ are separated by 89 nucleotides
b2305: yfcI - hypothetical protein (NCBI), at 2,420,671 to 2,421,561
yfcI
b2306: hisP - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,421,758 to 2,422,531
hisP
b2307: hisM - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,422,539 to 2,423,255
hisM
b2308: hisQ - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,423,252 to 2,423,938
hisQ
b2309: hisJ - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,424,028 to 2,424,810
hisJ
Position (kb)
2422
2423
2424 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2421.694 kb on + strand at 2421.771 kb on + strand at 2421.782 kb on - strand at 2421.790 kb on - strand at 2421.793 kb on - strand at 2421.793 kb on - strand at 2421.793 kb on - strand at 2421.793 kb on - strand at 2421.806 kb on - strand at 2421.860 kb on - strand, within hisP at 2421.874 kb on - strand, within hisP at 2421.918 kb on - strand, within hisP at 2421.921 kb on + strand, within hisP at 2421.928 kb on - strand, within hisP at 2421.928 kb on - strand, within hisP at 2421.963 kb on + strand, within hisP at 2421.963 kb on + strand, within hisP at 2421.971 kb on - strand, within hisP at 2421.973 kb on + strand, within hisP at 2421.981 kb on - strand, within hisP at 2421.981 kb on - strand, within hisP at 2422.005 kb on - strand, within hisP at 2422.199 kb on + strand, within hisP at 2422.199 kb on + strand, within hisP at 2422.218 kb on - strand, within hisP at 2422.232 kb on + strand, within hisP at 2422.232 kb on + strand, within hisP at 2422.255 kb on - strand, within hisP at 2422.255 kb on - strand, within hisP at 2422.375 kb on + strand, within hisP at 2422.486 kb on - strand at 2422.538 kb on - strand at 2422.538 kb on - strand at 2422.607 kb on + strand at 2422.607 kb on + strand at 2422.607 kb on + strand at 2422.621 kb on + strand, within hisM at 2422.627 kb on - strand, within hisM at 2422.684 kb on - strand, within hisM at 2422.684 kb on - strand, within hisM at 2422.684 kb on - strand, within hisM at 2422.711 kb on - strand, within hisM at 2422.712 kb on - strand, within hisM at 2422.730 kb on + strand, within hisM at 2422.738 kb on - strand, within hisM at 2422.740 kb on + strand, within hisM at 2422.740 kb on + strand, within hisM at 2422.765 kb on + strand, within hisM at 2422.765 kb on - strand, within hisM at 2422.788 kb on - strand, within hisM at 2422.798 kb on - strand, within hisM at 2422.836 kb on + strand, within hisM at 2422.850 kb on - strand, within hisM at 2422.850 kb on - strand, within hisM at 2422.904 kb on - strand, within hisM at 2422.942 kb on + strand, within hisM at 2422.947 kb on - strand, within hisM at 2422.962 kb on - strand, within hisM at 2423.022 kb on + strand, within hisM at 2423.038 kb on + strand, within hisM at 2423.041 kb on - strand, within hisM at 2423.145 kb on + strand, within hisM at 2423.145 kb on + strand, within hisM at 2423.199 kb on - strand at 2423.199 kb on - strand at 2423.233 kb on - strand at 2423.240 kb on - strand at 2423.240 kb on - strand at 2423.265 kb on - strand at 2423.265 kb on - strand at 2423.267 kb on + strand at 2423.280 kb on + strand at 2423.280 kb on + strand at 2423.310 kb on - strand at 2423.310 kb on - strand at 2423.346 kb on + strand, within hisQ at 2423.421 kb on - strand, within hisQ at 2423.426 kb on + strand, within hisQ at 2423.490 kb on + strand, within hisQ at 2423.566 kb on + strand, within hisQ at 2423.572 kb on - strand, within hisQ at 2423.641 kb on + strand, within hisQ at 2423.670 kb on + strand, within hisQ at 2423.696 kb on + strand, within hisQ at 2423.696 kb on + strand, within hisQ at 2423.702 kb on - strand, within hisQ at 2423.702 kb on - strand, within hisQ at 2423.752 kb on - strand, within hisQ at 2423.779 kb on + strand, within hisQ at 2423.786 kb on - strand, within hisQ at 2423.830 kb on - strand, within hisQ at 2423.830 kb on - strand, within hisQ at 2423.866 kb on + strand, within hisQ at 2423.866 kb on + strand, within hisQ at 2423.866 kb on + strand, within hisQ at 2423.866 kb on + strand, within hisQ at 2423.866 kb on + strand, within hisQ at 2423.940 kb on + strand at 2424.014 kb on - strand at 2424.158 kb on - strand, within hisJ at 2424.158 kb on - strand, within hisJ at 2424.158 kb on - strand, within hisJ at 2424.239 kb on - strand, within hisJ
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH23 in LB+Gent50 remove 2,421,694 + +0.3 2,421,771 + +2.0 2,421,782 - -0.1 2,421,790 - -0.2 2,421,793 - +1.0 2,421,793 - +0.3 2,421,793 - -0.4 2,421,793 - +0.5 2,421,806 - +0.6 2,421,860 - hisP b2306 0.13 +0.2 2,421,874 - hisP b2306 0.15 +0.1 2,421,918 - hisP b2306 0.21 -0.1 2,421,921 + hisP b2306 0.21 +2.0 2,421,928 - hisP b2306 0.22 +0.3 2,421,928 - hisP b2306 0.22 -0.2 2,421,963 + hisP b2306 0.26 +1.0 2,421,963 + hisP b2306 0.26 +2.4 2,421,971 - hisP b2306 0.28 -0.6 2,421,973 + hisP b2306 0.28 +1.0 2,421,981 - hisP b2306 0.29 -1.4 2,421,981 - hisP b2306 0.29 -0.1 2,422,005 - hisP b2306 0.32 -0.2 2,422,199 + hisP b2306 0.57 -0.1 2,422,199 + hisP b2306 0.57 +0.1 2,422,218 - hisP b2306 0.59 -0.1 2,422,232 + hisP b2306 0.61 -4.1 2,422,232 + hisP b2306 0.61 +0.4 2,422,255 - hisP b2306 0.64 -0.6 2,422,255 - hisP b2306 0.64 +0.4 2,422,375 + hisP b2306 0.80 -0.1 2,422,486 - -0.2 2,422,538 - +0.1 2,422,538 - +0.0 2,422,607 + -0.0 2,422,607 + -0.4 2,422,607 + +2.4 2,422,621 + hisM b2307 0.11 +0.3 2,422,627 - hisM b2307 0.12 +0.4 2,422,684 - hisM b2307 0.20 +0.7 2,422,684 - hisM b2307 0.20 +0.1 2,422,684 - hisM b2307 0.20 -0.3 2,422,711 - hisM b2307 0.24 -0.7 2,422,712 - hisM b2307 0.24 +0.1 2,422,730 + hisM b2307 0.27 -0.1 2,422,738 - hisM b2307 0.28 -0.0 2,422,740 + hisM b2307 0.28 -0.2 2,422,740 + hisM b2307 0.28 +0.4 2,422,765 + hisM b2307 0.32 -0.2 2,422,765 - hisM b2307 0.32 +0.4 2,422,788 - hisM b2307 0.35 +0.2 2,422,798 - hisM b2307 0.36 -0.1 2,422,836 + hisM b2307 0.41 -0.3 2,422,850 - hisM b2307 0.43 -0.0 2,422,850 - hisM b2307 0.43 -0.7 2,422,904 - hisM b2307 0.51 -0.1 2,422,942 + hisM b2307 0.56 +0.2 2,422,947 - hisM b2307 0.57 -0.2 2,422,962 - hisM b2307 0.59 -0.2 2,423,022 + hisM b2307 0.67 -0.4 2,423,038 + hisM b2307 0.70 +0.4 2,423,041 - hisM b2307 0.70 +1.1 2,423,145 + hisM b2307 0.85 +2.4 2,423,145 + hisM b2307 0.85 +0.3 2,423,199 - +0.5 2,423,199 - -0.5 2,423,233 - +0.0 2,423,240 - +0.5 2,423,240 - -0.1 2,423,265 - +0.1 2,423,265 - +0.0 2,423,267 + +0.3 2,423,280 + -1.0 2,423,280 + -0.6 2,423,310 - -0.9 2,423,310 - +0.6 2,423,346 + hisQ b2308 0.14 -0.4 2,423,421 - hisQ b2308 0.25 +0.1 2,423,426 + hisQ b2308 0.25 +0.1 2,423,490 + hisQ b2308 0.35 +0.7 2,423,566 + hisQ b2308 0.46 -0.1 2,423,572 - hisQ b2308 0.47 -0.8 2,423,641 + hisQ b2308 0.57 -0.3 2,423,670 + hisQ b2308 0.61 -0.0 2,423,696 + hisQ b2308 0.65 -0.3 2,423,696 + hisQ b2308 0.65 +0.2 2,423,702 - hisQ b2308 0.66 -0.6 2,423,702 - hisQ b2308 0.66 -0.3 2,423,752 - hisQ b2308 0.73 -0.2 2,423,779 + hisQ b2308 0.77 -0.1 2,423,786 - hisQ b2308 0.78 -0.1 2,423,830 - hisQ b2308 0.84 -0.3 2,423,830 - hisQ b2308 0.84 -0.5 2,423,866 + hisQ b2308 0.89 -0.4 2,423,866 + hisQ b2308 0.89 +0.2 2,423,866 + hisQ b2308 0.89 +0.3 2,423,866 + hisQ b2308 0.89 +0.2 2,423,866 + hisQ b2308 0.89 +0.4 2,423,940 + +0.2 2,424,014 - +0.0 2,424,158 - hisJ b2309 0.17 -0.4 2,424,158 - hisJ b2309 0.17 -0.1 2,424,158 - hisJ b2309 0.17 -0.6 2,424,239 - hisJ b2309 0.27 -0.3
Or see this region's nucleotide sequence