Experiment: Ying_Nucleobase14 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdoH and yceK are separated by 172 nucleotides yceK and msyB are separated by 0 nucleotides msyB and mdtG are separated by 82 nucleotides mdtG and lpxL are separated by 171 nucleotides
b1049: mdoH - glucosyltransferase MdoH (NCBI), at 1,110,086 to 1,112,629
mdoH
b1050: yceK - predicted lipoprotein (NCBI), at 1,112,802 to 1,113,029
yceK
b1051: msyB - acidic protein suppresses mutants lacking function of protein export (VIMSS), at 1,113,030 to 1,113,404
msyB
b1053: mdtG - predicted drug efflux system (NCBI), at 1,113,487 to 1,114,713
mdtG
b1054: lpxL - lipid A biosynthesis lauroyl acyltransferase (NCBI), at 1,114,885 to 1,115,805
lpxL
Position (kb)
1113
1114
1115 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1112.561 kb on - strand at 1112.670 kb on - strand at 1112.866 kb on + strand, within yceK at 1113.040 kb on + strand at 1113.040 kb on + strand at 1113.311 kb on - strand, within msyB at 1113.311 kb on - strand, within msyB at 1113.450 kb on - strand at 1113.524 kb on - strand at 1113.533 kb on + strand at 1113.533 kb on + strand at 1113.665 kb on - strand at 1113.703 kb on - strand, within mdtG at 1113.816 kb on + strand, within mdtG at 1113.893 kb on - strand, within mdtG at 1113.915 kb on + strand, within mdtG at 1113.915 kb on + strand, within mdtG at 1114.083 kb on - strand, within mdtG at 1114.083 kb on - strand, within mdtG at 1114.104 kb on + strand, within mdtG at 1114.136 kb on + strand, within mdtG at 1114.136 kb on + strand, within mdtG at 1114.274 kb on + strand, within mdtG at 1114.411 kb on + strand, within mdtG at 1114.411 kb on + strand, within mdtG at 1114.437 kb on + strand, within mdtG at 1114.508 kb on - strand, within mdtG at 1114.600 kb on - strand at 1114.600 kb on - strand at 1114.703 kb on - strand at 1114.833 kb on + strand at 1114.846 kb on - strand at 1114.889 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3 remove 1,112,561 - -0.2 1,112,670 - +0.1 1,112,866 + yceK b1050 0.28 -0.7 1,113,040 + +2.2 1,113,040 + +0.7 1,113,311 - msyB b1051 0.75 +0.2 1,113,311 - msyB b1051 0.75 +0.7 1,113,450 - +0.1 1,113,524 - -0.2 1,113,533 + -0.2 1,113,533 + +1.3 1,113,665 - -0.7 1,113,703 - mdtG b1053 0.18 +0.2 1,113,816 + mdtG b1053 0.27 -0.6 1,113,893 - mdtG b1053 0.33 +0.3 1,113,915 + mdtG b1053 0.35 -0.3 1,113,915 + mdtG b1053 0.35 +0.6 1,114,083 - mdtG b1053 0.49 -0.3 1,114,083 - mdtG b1053 0.49 +0.2 1,114,104 + mdtG b1053 0.50 +0.1 1,114,136 + mdtG b1053 0.53 +0.5 1,114,136 + mdtG b1053 0.53 -0.4 1,114,274 + mdtG b1053 0.64 -0.0 1,114,411 + mdtG b1053 0.75 -0.1 1,114,411 + mdtG b1053 0.75 +0.7 1,114,437 + mdtG b1053 0.77 +0.5 1,114,508 - mdtG b1053 0.83 -4.6 1,114,600 - +0.0 1,114,600 - -0.0 1,114,703 - -0.4 1,114,833 + -0.0 1,114,846 - +0.1 1,114,889 - +0.5
Or see this region's nucleotide sequence