Experiment: Ying_sugar7 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpC and trpD are separated by 3 nucleotides trpD and trpE overlap by 1 nucleotides
b1262: trpC - N-(5-phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthetase (VIMSS), at 1,316,451 to 1,317,809
trpC
b1263: trpD - bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI), at 1,317,813 to 1,319,408
trpD
b1264: trpE - anthranilate synthase component I (NCBI), at 1,319,408 to 1,320,970
trpE
Position (kb)
1317
1318
1319
1320 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1317.035 kb on - strand, within trpC at 1317.035 kb on - strand, within trpC at 1317.113 kb on - strand, within trpC at 1317.144 kb on - strand, within trpC at 1317.153 kb on + strand, within trpC at 1317.181 kb on + strand, within trpC at 1317.305 kb on - strand, within trpC at 1317.389 kb on + strand, within trpC at 1317.389 kb on + strand, within trpC at 1317.643 kb on + strand, within trpC at 1317.684 kb on - strand at 1317.684 kb on - strand at 1317.766 kb on - strand at 1318.316 kb on + strand, within trpD at 1318.316 kb on + strand, within trpD at 1319.016 kb on - strand, within trpD at 1319.138 kb on - strand, within trpD at 1319.228 kb on + strand, within trpD at 1319.372 kb on - strand at 1319.455 kb on - strand at 1319.714 kb on + strand, within trpE at 1319.820 kb on + strand, within trpE at 1320.027 kb on + strand, within trpE at 1320.044 kb on + strand, within trpE at 1320.100 kb on - strand, within trpE at 1320.119 kb on - strand, within trpE at 1320.125 kb on + strand, within trpE at 1320.125 kb on + strand, within trpE at 1320.275 kb on - strand, within trpE at 1320.275 kb on - strand, within trpE
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 3 remove 1,317,035 - trpC b1262 0.43 -3.4 1,317,035 - trpC b1262 0.43 -3.0 1,317,113 - trpC b1262 0.49 -1.7 1,317,144 - trpC b1262 0.51 -6.9 1,317,153 + trpC b1262 0.52 -5.7 1,317,181 + trpC b1262 0.54 -5.3 1,317,305 - trpC b1262 0.63 -2.9 1,317,389 + trpC b1262 0.69 -4.2 1,317,389 + trpC b1262 0.69 -4.3 1,317,643 + trpC b1262 0.88 -5.5 1,317,684 - -5.0 1,317,684 - -3.9 1,317,766 - -3.1 1,318,316 + trpD b1263 0.32 -4.4 1,318,316 + trpD b1263 0.32 -4.0 1,319,016 - trpD b1263 0.75 -4.1 1,319,138 - trpD b1263 0.83 -4.6 1,319,228 + trpD b1263 0.89 -2.7 1,319,372 - -2.8 1,319,455 - -4.1 1,319,714 + trpE b1264 0.20 -3.4 1,319,820 + trpE b1264 0.26 -5.7 1,320,027 + trpE b1264 0.40 -4.6 1,320,044 + trpE b1264 0.41 -6.2 1,320,100 - trpE b1264 0.44 -4.5 1,320,119 - trpE b1264 0.45 -1.9 1,320,125 + trpE b1264 0.46 -4.4 1,320,125 + trpE b1264 0.46 -3.4 1,320,275 - trpE b1264 0.55 -4.9 1,320,275 - trpE b1264 0.55 -5.2
Or see this region's nucleotide sequence