Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuC and leuB are separated by 2 nucleotides leuB and leuA overlap by 1 nucleotides
b0072: leuC - isopropylmalate isomerase large subunit (NCBI), at 79,464 to 80,864
leuC
b0073: leuB - 3-isopropylmalate dehydrogenase (VIMSS), at 80,867 to 81,958
leuB
b0074: leuA - 2-isopropylmalate synthase (NCBI), at 81,958 to 83,529
leuA
Position (kb)
80
81
82 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 79.883 kb on + strand, within leuC at 79.888 kb on + strand, within leuC at 79.896 kb on - strand, within leuC at 79.931 kb on + strand, within leuC at 80.049 kb on - strand, within leuC at 80.049 kb on - strand, within leuC at 80.077 kb on + strand, within leuC at 80.077 kb on + strand, within leuC at 80.092 kb on - strand, within leuC at 80.120 kb on - strand, within leuC at 80.120 kb on - strand, within leuC at 80.141 kb on + strand, within leuC at 80.141 kb on + strand, within leuC at 80.165 kb on + strand, within leuC at 80.210 kb on + strand, within leuC at 80.210 kb on + strand, within leuC at 80.218 kb on - strand, within leuC at 80.218 kb on - strand, within leuC at 80.236 kb on - strand, within leuC at 80.349 kb on + strand, within leuC at 80.387 kb on + strand at 80.387 kb on + strand, within leuC at 80.414 kb on - strand, within leuC at 80.438 kb on - strand, within leuC at 80.671 kb on - strand, within leuC at 80.671 kb on - strand, within leuC at 80.671 kb on - strand, within leuC at 80.671 kb on - strand, within leuC at 80.714 kb on - strand, within leuC at 80.714 kb on - strand, within leuC at 80.785 kb on + strand at 80.785 kb on + strand at 80.893 kb on - strand at 80.893 kb on - strand at 80.918 kb on + strand at 80.918 kb on + strand at 80.929 kb on - strand at 80.965 kb on + strand at 80.973 kb on - strand at 80.986 kb on - strand, within leuB at 81.032 kb on - strand, within leuB at 81.037 kb on + strand, within leuB at 81.114 kb on + strand, within leuB at 81.114 kb on + strand, within leuB at 81.122 kb on - strand, within leuB at 81.191 kb on - strand, within leuB at 81.191 kb on - strand, within leuB at 81.224 kb on + strand, within leuB at 81.284 kb on + strand, within leuB at 81.284 kb on + strand, within leuB at 81.287 kb on + strand, within leuB at 81.290 kb on - strand, within leuB at 81.292 kb on - strand, within leuB at 81.292 kb on - strand, within leuB at 81.314 kb on - strand, within leuB at 81.404 kb on + strand, within leuB at 81.409 kb on - strand, within leuB at 81.457 kb on + strand, within leuB at 81.480 kb on + strand, within leuB at 81.554 kb on - strand, within leuB at 81.580 kb on + strand, within leuB at 81.607 kb on - strand, within leuB at 81.692 kb on + strand, within leuB at 81.692 kb on + strand, within leuB at 81.910 kb on + strand at 81.910 kb on + strand at 81.922 kb on - strand at 82.051 kb on + strand at 82.135 kb on + strand, within leuA at 82.243 kb on + strand, within leuA at 82.243 kb on + strand, within leuA at 82.246 kb on - strand, within leuA at 82.425 kb on + strand, within leuA at 82.462 kb on - strand, within leuA at 82.462 kb on - strand, within leuA at 82.502 kb on - strand, within leuA at 82.506 kb on - strand, within leuA at 82.624 kb on + strand, within leuA at 82.632 kb on - strand, within leuA at 82.635 kb on + strand, within leuA at 82.637 kb on + strand, within leuA at 82.638 kb on - strand, within leuA at 82.638 kb on - strand, within leuA at 82.647 kb on + strand, within leuA at 82.671 kb on - strand, within leuA at 82.671 kb on - strand, within leuA at 82.671 kb on - strand, within leuA at 82.713 kb on + strand, within leuA at 82.860 kb on + strand, within leuA at 82.860 kb on + strand, within leuA at 82.870 kb on + strand, within leuA at 82.878 kb on - strand, within leuA
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 79,883 + leuC b0072 0.30 -5.9 79,888 + leuC b0072 0.30 -6.0 79,896 - leuC b0072 0.31 -5.0 79,931 + leuC b0072 0.33 -2.7 80,049 - leuC b0072 0.42 -4.3 80,049 - leuC b0072 0.42 -5.5 80,077 + leuC b0072 0.44 -6.7 80,077 + leuC b0072 0.44 -5.8 80,092 - leuC b0072 0.45 -5.2 80,120 - leuC b0072 0.47 -5.4 80,120 - leuC b0072 0.47 -4.7 80,141 + leuC b0072 0.48 -4.4 80,141 + leuC b0072 0.48 -3.2 80,165 + leuC b0072 0.50 -2.5 80,210 + leuC b0072 0.53 -3.8 80,210 + leuC b0072 0.53 -3.4 80,218 - leuC b0072 0.54 -5.7 80,218 - leuC b0072 0.54 -5.1 80,236 - leuC b0072 0.55 -5.3 80,349 + leuC b0072 0.63 -5.1 80,387 + -2.1 80,387 + leuC b0072 0.66 -3.7 80,414 - leuC b0072 0.68 -6.0 80,438 - leuC b0072 0.70 -4.4 80,671 - leuC b0072 0.86 -5.0 80,671 - leuC b0072 0.86 -4.3 80,671 - leuC b0072 0.86 -4.9 80,671 - leuC b0072 0.86 -5.1 80,714 - leuC b0072 0.89 -3.7 80,714 - leuC b0072 0.89 -3.2 80,785 + +0.3 80,785 + -3.2 80,893 - -3.1 80,893 - -3.3 80,918 + -5.3 80,918 + -3.3 80,929 - -4.1 80,965 + -3.9 80,973 - -4.9 80,986 - leuB b0073 0.11 -6.0 81,032 - leuB b0073 0.15 -4.1 81,037 + leuB b0073 0.16 -4.7 81,114 + leuB b0073 0.23 -2.1 81,114 + leuB b0073 0.23 -4.2 81,122 - leuB b0073 0.23 -5.0 81,191 - leuB b0073 0.30 -5.4 81,191 - leuB b0073 0.30 -4.4 81,224 + leuB b0073 0.33 -5.6 81,284 + leuB b0073 0.38 -3.3 81,284 + leuB b0073 0.38 -2.6 81,287 + leuB b0073 0.38 -3.7 81,290 - leuB b0073 0.39 -5.0 81,292 - leuB b0073 0.39 -6.3 81,292 - leuB b0073 0.39 -3.4 81,314 - leuB b0073 0.41 -4.8 81,404 + leuB b0073 0.49 -4.5 81,409 - leuB b0073 0.50 -3.3 81,457 + leuB b0073 0.54 -5.5 81,480 + leuB b0073 0.56 -3.8 81,554 - leuB b0073 0.63 -5.2 81,580 + leuB b0073 0.65 -5.8 81,607 - leuB b0073 0.68 -3.7 81,692 + leuB b0073 0.76 -3.4 81,692 + leuB b0073 0.76 -6.5 81,910 + -5.4 81,910 + -3.8 81,922 - -6.2 82,051 + -3.2 82,135 + leuA b0074 0.11 -4.0 82,243 + leuA b0074 0.18 -2.5 82,243 + leuA b0074 0.18 -3.9 82,246 - leuA b0074 0.18 -3.0 82,425 + leuA b0074 0.30 -4.0 82,462 - leuA b0074 0.32 -3.7 82,462 - leuA b0074 0.32 -3.3 82,502 - leuA b0074 0.35 -5.7 82,506 - leuA b0074 0.35 -4.1 82,624 + leuA b0074 0.42 -5.3 82,632 - leuA b0074 0.43 -5.4 82,635 + leuA b0074 0.43 -5.5 82,637 + leuA b0074 0.43 -4.6 82,638 - leuA b0074 0.43 -5.6 82,638 - leuA b0074 0.43 -4.0 82,647 + leuA b0074 0.44 -5.5 82,671 - leuA b0074 0.45 -4.1 82,671 - leuA b0074 0.45 -6.1 82,671 - leuA b0074 0.45 -4.3 82,713 + leuA b0074 0.48 -3.1 82,860 + leuA b0074 0.57 -2.4 82,860 + leuA b0074 0.57 -2.2 82,870 + leuA b0074 0.58 -5.7 82,878 - leuA b0074 0.59 -3.4
Or see this region's nucleotide sequence