Experiment: Ying_Others16 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt upp and purM are separated by 324 nucleotides purM and purN overlap by 1 nucleotides purN and ppk are separated by 171 nucleotides
b2498: upp - uracil phosphoribosyltransferase (VIMSS), at 2,618,268 to 2,618,894
upp
b2499: purM - phosphoribosylaminoimidazole synthetase (NCBI), at 2,619,219 to 2,620,256
purM
b2500: purN - phosphoribosylglycinamide formyltransferase (NCBI), at 2,620,256 to 2,620,894
purN
b2501: ppk - polyphosphate kinase (NCBI), at 2,621,066 to 2,623,132
ppk
Position (kb)
2619
2620
2621 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2618.356 kb on + strand, within upp at 2618.361 kb on - strand, within upp at 2618.361 kb on - strand, within upp at 2618.361 kb on - strand, within upp at 2618.364 kb on - strand, within upp at 2618.402 kb on + strand, within upp at 2618.561 kb on + strand, within upp at 2618.648 kb on + strand, within upp at 2618.648 kb on + strand, within upp at 2618.729 kb on - strand, within upp at 2618.770 kb on + strand, within upp at 2618.778 kb on - strand, within upp at 2618.846 kb on + strand at 2618.889 kb on - strand at 2619.127 kb on + strand at 2619.127 kb on + strand at 2619.262 kb on + strand at 2619.284 kb on + strand at 2619.362 kb on + strand, within purM at 2619.501 kb on - strand, within purM at 2619.662 kb on + strand, within purM at 2619.662 kb on + strand, within purM at 2619.832 kb on + strand, within purM at 2619.832 kb on + strand, within purM at 2619.835 kb on - strand, within purM at 2619.923 kb on - strand, within purM at 2619.923 kb on - strand, within purM at 2619.971 kb on - strand, within purM at 2620.035 kb on + strand, within purM at 2620.035 kb on + strand, within purM at 2620.043 kb on - strand, within purM at 2620.164 kb on + strand at 2620.164 kb on + strand at 2620.164 kb on + strand at 2620.175 kb on + strand at 2620.258 kb on + strand at 2620.258 kb on + strand at 2620.260 kb on + strand at 2620.422 kb on - strand, within purN at 2620.451 kb on + strand, within purN at 2620.459 kb on - strand, within purN at 2620.470 kb on - strand, within purN at 2620.604 kb on + strand, within purN at 2620.651 kb on + strand, within purN at 2620.651 kb on + strand, within purN at 2620.661 kb on - strand, within purN at 2620.744 kb on + strand, within purN at 2620.873 kb on + strand at 2620.873 kb on + strand at 2620.899 kb on - strand at 2620.981 kb on + strand at 2620.981 kb on + strand at 2620.989 kb on - strand at 2620.989 kb on - strand at 2620.998 kb on - strand at 2621.024 kb on - strand at 2621.032 kb on - strand at 2621.041 kb on - strand at 2621.118 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 1 remove 2,618,356 + upp b2498 0.14 -0.8 2,618,361 - upp b2498 0.15 +1.3 2,618,361 - upp b2498 0.15 -2.5 2,618,361 - upp b2498 0.15 +0.7 2,618,364 - upp b2498 0.15 +1.7 2,618,402 + upp b2498 0.21 -0.2 2,618,561 + upp b2498 0.47 -1.5 2,618,648 + upp b2498 0.61 -1.9 2,618,648 + upp b2498 0.61 +1.3 2,618,729 - upp b2498 0.74 +0.9 2,618,770 + upp b2498 0.80 -0.9 2,618,778 - upp b2498 0.81 +0.1 2,618,846 + -1.2 2,618,889 - -0.8 2,619,127 + +0.4 2,619,127 + +0.0 2,619,262 + -0.5 2,619,284 + -0.5 2,619,362 + purM b2499 0.14 -0.6 2,619,501 - purM b2499 0.27 -0.4 2,619,662 + purM b2499 0.43 -0.7 2,619,662 + purM b2499 0.43 -0.1 2,619,832 + purM b2499 0.59 -0.3 2,619,832 + purM b2499 0.59 -0.9 2,619,835 - purM b2499 0.59 -0.8 2,619,923 - purM b2499 0.68 -0.1 2,619,923 - purM b2499 0.68 -1.0 2,619,971 - purM b2499 0.72 -0.7 2,620,035 + purM b2499 0.79 -1.0 2,620,035 + purM b2499 0.79 -2.1 2,620,043 - purM b2499 0.79 -0.5 2,620,164 + -1.3 2,620,164 + +1.8 2,620,164 + -0.5 2,620,175 + -0.1 2,620,258 + -1.1 2,620,258 + -0.7 2,620,260 + +0.0 2,620,422 - purN b2500 0.26 +0.1 2,620,451 + purN b2500 0.31 +1.1 2,620,459 - purN b2500 0.32 -0.5 2,620,470 - purN b2500 0.33 +0.3 2,620,604 + purN b2500 0.54 +0.0 2,620,651 + purN b2500 0.62 -0.6 2,620,651 + purN b2500 0.62 +0.0 2,620,661 - purN b2500 0.63 -0.7 2,620,744 + purN b2500 0.76 +0.1 2,620,873 + -0.3 2,620,873 + -0.7 2,620,899 - -0.8 2,620,981 + +0.2 2,620,981 + +1.0 2,620,989 - +0.0 2,620,989 - +0.2 2,620,998 - +0.4 2,621,024 - +0.5 2,621,032 - -0.5 2,621,041 - +1.2 2,621,118 - -1.5
Or see this region's nucleotide sequence