Experiment: Ying_sugar7 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fadH and ygjM are separated by 44 nucleotides ygjM and ygjN overlap by 4 nucleotides ygjN and ygjO are separated by 283 nucleotides
b3081: fadH - 2,4-dienoyl-CoA reductase, NADH and FMN-linked (NCBI), at 3,229,687 to 3,231,705
fadH
b3082: ygjM - predicted DNA-binding transcriptional regulator (NCBI), at 3,231,750 to 3,232,166
ygjM
b3083: ygjN - hypothetical protein (NCBI), at 3,232,163 to 3,232,477
ygjN
b3084: ygjO - putative enzyme (VIMSS), at 3,232,761 to 3,233,897
ygjO
Position (kb)
3231
3232
3233 Strain fitness (log2 ratio)
-1
0
1 at 3230.922 kb on + strand, within fadH at 3230.928 kb on - strand, within fadH at 3231.019 kb on + strand, within fadH at 3231.139 kb on + strand, within fadH at 3231.163 kb on - strand, within fadH at 3231.199 kb on - strand, within fadH at 3231.267 kb on + strand, within fadH at 3231.267 kb on + strand, within fadH at 3231.356 kb on + strand, within fadH at 3231.399 kb on - strand, within fadH at 3231.427 kb on - strand, within fadH at 3231.428 kb on + strand, within fadH at 3231.428 kb on + strand, within fadH at 3231.428 kb on + strand, within fadH at 3231.428 kb on + strand, within fadH at 3231.504 kb on - strand at 3231.530 kb on + strand at 3231.530 kb on + strand at 3231.547 kb on + strand at 3231.552 kb on + strand at 3231.643 kb on + strand at 3231.673 kb on + strand at 3231.812 kb on - strand, within ygjM at 3231.855 kb on - strand, within ygjM at 3231.856 kb on - strand, within ygjM at 3231.892 kb on - strand, within ygjM at 3231.892 kb on - strand, within ygjM at 3232.179 kb on + strand at 3232.179 kb on + strand at 3232.181 kb on + strand at 3232.186 kb on + strand at 3232.189 kb on - strand at 3232.233 kb on + strand, within ygjN at 3232.267 kb on + strand, within ygjN at 3232.272 kb on - strand, within ygjN at 3232.302 kb on + strand, within ygjN at 3232.394 kb on + strand, within ygjN at 3232.416 kb on + strand, within ygjN at 3232.465 kb on - strand at 3232.465 kb on - strand at 3232.465 kb on - strand at 3232.471 kb on + strand at 3232.603 kb on + strand at 3232.603 kb on + strand at 3232.704 kb on - strand at 3232.704 kb on - strand at 3232.783 kb on + strand at 3232.783 kb on + strand at 3232.815 kb on + strand at 3232.830 kb on - strand at 3232.862 kb on + strand at 3232.862 kb on + strand at 3232.924 kb on - strand, within ygjO at 3232.967 kb on - strand, within ygjO at 3232.979 kb on - strand, within ygjO at 3232.979 kb on - strand, within ygjO at 3232.999 kb on - strand, within ygjO at 3232.999 kb on - strand, within ygjO
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 1 remove 3,230,922 + fadH b3081 0.61 -0.1 3,230,928 - fadH b3081 0.61 +0.3 3,231,019 + fadH b3081 0.66 +0.5 3,231,139 + fadH b3081 0.72 -0.4 3,231,163 - fadH b3081 0.73 +0.2 3,231,199 - fadH b3081 0.75 -0.2 3,231,267 + fadH b3081 0.78 -1.6 3,231,267 + fadH b3081 0.78 +0.4 3,231,356 + fadH b3081 0.83 +0.1 3,231,399 - fadH b3081 0.85 +0.1 3,231,427 - fadH b3081 0.86 +0.4 3,231,428 + fadH b3081 0.86 -0.2 3,231,428 + fadH b3081 0.86 -0.2 3,231,428 + fadH b3081 0.86 -0.1 3,231,428 + fadH b3081 0.86 -0.5 3,231,504 - +0.8 3,231,530 + -0.6 3,231,530 + -0.0 3,231,547 + +0.6 3,231,552 + -0.8 3,231,643 + -0.9 3,231,673 + -0.3 3,231,812 - ygjM b3082 0.15 -0.4 3,231,855 - ygjM b3082 0.25 -0.6 3,231,856 - ygjM b3082 0.25 -0.6 3,231,892 - ygjM b3082 0.34 +1.0 3,231,892 - ygjM b3082 0.34 -0.6 3,232,179 + -0.5 3,232,179 + -0.2 3,232,181 + +1.4 3,232,186 + +0.2 3,232,189 - -0.9 3,232,233 + ygjN b3083 0.22 +0.0 3,232,267 + ygjN b3083 0.33 -0.1 3,232,272 - ygjN b3083 0.35 -0.1 3,232,302 + ygjN b3083 0.44 -0.1 3,232,394 + ygjN b3083 0.73 +0.3 3,232,416 + ygjN b3083 0.80 -0.9 3,232,465 - +0.1 3,232,465 - -0.4 3,232,465 - +0.3 3,232,471 + -1.4 3,232,603 + +0.1 3,232,603 + -0.3 3,232,704 - -0.5 3,232,704 - -0.3 3,232,783 + -0.9 3,232,783 + +0.1 3,232,815 + +0.2 3,232,830 - -0.1 3,232,862 + -0.4 3,232,862 + -0.0 3,232,924 - ygjO b3084 0.14 -0.3 3,232,967 - ygjO b3084 0.18 +0.1 3,232,979 - ygjO b3084 0.19 -0.0 3,232,979 - ygjO b3084 0.19 +0.5 3,232,999 - ygjO b3084 0.21 +0.0 3,232,999 - ygjO b3084 0.21 +0.3
Or see this region's nucleotide sequence