Experiment: vcDART vector transformation; agar assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt epd and yggC are separated by 284 nucleotides yggC and yggD overlap by 4 nucleotides yggD and yggF are separated by 21 nucleotides yggF and yggP overlap by 4 nucleotides
b2927: epd - D-erythrose 4-phosphate dehydrogenase (NCBI), at 3,070,694 to 3,071,713
epd
b2928: yggC - hypothetical protein (NCBI), at 3,071,998 to 3,072,711
yggC
b2929: yggD - predicted DNA-binding transcriptional regulator (NCBI), at 3,072,708 to 3,073,217
yggD
b2930: yggF - predicted hexoseP phosphatase (NCBI), at 3,073,239 to 3,074,204
yggF
b4465: yggP - predicted dehydrogenase (NCBI), at 3,074,201 to 3,075,478
yggP
Position (kb)
3072
3073
3074 Strain fitness (log2 ratio)
-1
0
1 at 3071.717 kb on - strand at 3071.763 kb on - strand at 3071.763 kb on - strand at 3071.942 kb on - strand at 3071.946 kb on + strand at 3071.954 kb on - strand at 3072.064 kb on - strand at 3072.064 kb on - strand at 3072.088 kb on - strand, within yggC at 3072.160 kb on - strand, within yggC at 3072.240 kb on - strand, within yggC at 3072.240 kb on - strand, within yggC at 3072.340 kb on + strand, within yggC at 3072.340 kb on + strand, within yggC at 3072.367 kb on - strand, within yggC at 3072.434 kb on - strand, within yggC at 3072.495 kb on + strand, within yggC at 3072.495 kb on + strand, within yggC at 3072.551 kb on + strand, within yggC at 3072.551 kb on + strand, within yggC at 3072.578 kb on + strand, within yggC at 3072.612 kb on - strand, within yggC at 3072.671 kb on + strand at 3072.672 kb on - strand at 3072.695 kb on - strand at 3072.695 kb on - strand at 3072.827 kb on + strand, within yggD at 3072.856 kb on + strand, within yggD at 3072.939 kb on - strand, within yggD at 3072.969 kb on - strand, within yggD at 3073.062 kb on + strand, within yggD at 3073.062 kb on + strand, within yggD at 3073.174 kb on + strand at 3073.204 kb on - strand at 3073.204 kb on - strand at 3073.263 kb on - strand at 3073.272 kb on - strand at 3073.322 kb on + strand at 3073.328 kb on - strand at 3073.330 kb on - strand at 3073.414 kb on - strand, within yggF at 3073.518 kb on + strand, within yggF at 3073.518 kb on + strand, within yggF at 3073.714 kb on + strand, within yggF at 3073.729 kb on + strand, within yggF at 3073.729 kb on + strand, within yggF at 3073.922 kb on - strand, within yggF at 3073.922 kb on - strand, within yggF at 3074.056 kb on - strand, within yggF at 3074.081 kb on - strand, within yggF at 3074.204 kb on - strand at 3074.204 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction vcDART vector transformation; agar assay remove 3,071,717 - +0.5 3,071,763 - +0.1 3,071,763 - -0.5 3,071,942 - +0.1 3,071,946 + -0.2 3,071,954 - -0.4 3,072,064 - +0.2 3,072,064 - +0.6 3,072,088 - yggC b2928 0.13 -0.6 3,072,160 - yggC b2928 0.23 +0.3 3,072,240 - yggC b2928 0.34 -0.3 3,072,240 - yggC b2928 0.34 -0.3 3,072,340 + yggC b2928 0.48 +0.4 3,072,340 + yggC b2928 0.48 -0.2 3,072,367 - yggC b2928 0.52 -0.1 3,072,434 - yggC b2928 0.61 -0.4 3,072,495 + yggC b2928 0.70 +1.6 3,072,495 + yggC b2928 0.70 -1.3 3,072,551 + yggC b2928 0.77 +0.2 3,072,551 + yggC b2928 0.77 -0.3 3,072,578 + yggC b2928 0.81 -0.2 3,072,612 - yggC b2928 0.86 -1.1 3,072,671 + -0.6 3,072,672 - -0.1 3,072,695 - -0.8 3,072,695 - -0.0 3,072,827 + yggD b2929 0.23 -0.5 3,072,856 + yggD b2929 0.29 -0.1 3,072,939 - yggD b2929 0.45 +0.6 3,072,969 - yggD b2929 0.51 -0.1 3,073,062 + yggD b2929 0.69 -1.0 3,073,062 + yggD b2929 0.69 -0.7 3,073,174 + +0.2 3,073,204 - -0.2 3,073,204 - -1.2 3,073,263 - -0.0 3,073,272 - +0.3 3,073,322 + -1.4 3,073,328 - -0.5 3,073,330 - +0.2 3,073,414 - yggF b2930 0.18 -0.2 3,073,518 + yggF b2930 0.29 +0.4 3,073,518 + yggF b2930 0.29 +0.1 3,073,714 + yggF b2930 0.49 +0.9 3,073,729 + yggF b2930 0.51 -0.4 3,073,729 + yggF b2930 0.51 -0.6 3,073,922 - yggF b2930 0.71 -1.0 3,073,922 - yggF b2930 0.71 -0.0 3,074,056 - yggF b2930 0.85 -0.7 3,074,081 - yggF b2930 0.87 +0.8 3,074,204 - +0.1 3,074,204 - +1.5
Or see this region's nucleotide sequence