Strain Fitness in Pseudomonas putida KT2440 around PP_0123

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPP_0121 and PP_0122 are separated by 69 nucleotidesPP_0122 and PP_0123 are separated by 75 nucleotidesPP_0123 and PP_0124 are separated by 370 nucleotides PP_0121: PP_0121 - homoserine kinase, at 126,140 to 127,090 _0121 PP_0122: PP_0122 - conserved exported protein of unknown function, at 127,160 to 127,447 _0122 PP_0123: PP_0123 - DNA polymerase I, 5, at 127,523 to 130,270 _0123 PP_0124: PP_0124 - probable GTP-binding protein, at 130,641 to 131,273 _0124 Position (kb) 127 128 129 130 131Strain fitness (log2 ratio) -2 -1 0 1at 126.629 kb on + strandat 127.162 kb on - strandat 127.291 kb on - strand, within PP_0122at 127.291 kb on - strand, within PP_0122at 127.312 kb on - strand, within PP_0122at 127.327 kb on + strand, within PP_0122at 127.328 kb on - strand, within PP_0122at 127.424 kb on + strandat 127.425 kb on - strandat 127.450 kb on + strandat 127.455 kb on + strandat 127.455 kb on + strandat 127.455 kb on + strandat 127.456 kb on - strandat 128.462 kb on + strand, within PP_0123at 128.463 kb on - strand, within PP_0123at 128.463 kb on - strand, within PP_0123at 128.618 kb on + strand, within PP_0123at 128.618 kb on + strandat 128.619 kb on - strand, within PP_0123at 128.619 kb on - strand, within PP_0123at 128.619 kb on - strand, within PP_0123at 128.639 kb on + strand, within PP_0123at 128.742 kb on - strand, within PP_0123at 128.819 kb on + strand, within PP_0123at 128.819 kb on + strand, within PP_0123at 128.819 kb on + strand, within PP_0123at 128.820 kb on - strand, within PP_0123at 128.820 kb on - strand, within PP_0123at 128.873 kb on + strand, within PP_0123at 128.932 kb on + strand, within PP_0123at 128.932 kb on + strand, within PP_0123at 128.933 kb on - strand, within PP_0123at 128.970 kb on - strand, within PP_0123at 129.141 kb on + strand, within PP_0123at 129.191 kb on + strand, within PP_0123at 129.192 kb on - strand, within PP_0123at 129.371 kb on + strand, within PP_0123at 129.372 kb on - strand, within PP_0123at 129.401 kb on + strand, within PP_0123at 129.401 kb on + strand, within PP_0123at 129.401 kb on + strand, within PP_0123at 129.402 kb on - strand, within PP_0123at 129.414 kb on + strand, within PP_0123at 129.414 kb on + strand, within PP_0123at 129.415 kb on - strand, within PP_0123at 129.458 kb on + strand, within PP_0123at 129.458 kb on + strand, within PP_0123at 129.578 kb on + strand, within PP_0123at 129.599 kb on + strand, within PP_0123at 129.599 kb on + strand, within PP_0123at 129.599 kb on + strand, within PP_0123at 129.600 kb on - strand, within PP_0123at 129.619 kb on + strand, within PP_0123at 129.620 kb on - strand, within PP_0123at 129.620 kb on - strand, within PP_0123at 129.779 kb on + strand, within PP_0123at 129.779 kb on + strand, within PP_0123at 129.780 kb on - strand, within PP_0123at 129.842 kb on + strand, within PP_0123at 129.842 kb on + strand, within PP_0123at 129.842 kb on + strand, within PP_0123at 129.842 kb on + strand, within PP_0123at 129.843 kb on - strand, within PP_0123at 129.843 kb on - strand, within PP_0123at 129.843 kb on - strand, within PP_0123at 129.844 kb on + strand, within PP_0123at 129.845 kb on - strand, within PP_0123at 129.845 kb on - strand, within PP_0123at 129.845 kb on - strandat 129.845 kb on - strand, within PP_0123at 129.854 kb on + strand, within PP_0123at 129.884 kb on + strand, within PP_0123at 129.885 kb on - strand, within PP_0123at 129.885 kb on - strand, within PP_0123at 129.953 kb on + strand, within PP_0123at 129.954 kb on - strand, within PP_0123at 130.071 kb on + strandat 130.075 kb on + strandat 130.075 kb on + strandat 130.076 kb on - strandat 130.146 kb on + strandat 130.147 kb on - strandat 130.291 kb on - strandat 130.304 kb on + strandat 130.305 kb on - strandat 130.345 kb on + strandat 130.345 kb on + strandat 130.346 kb on - strandat 130.346 kb on - strandat 130.370 kb on - strandat 130.390 kb on + strandat 130.391 kb on - strandat 130.391 kb on - strandat 130.415 kb on + strandat 130.449 kb on + strandat 130.449 kb on + strandat 130.449 kb on + strandat 130.450 kb on - strandat 130.450 kb on - strandat 130.450 kb on - strandat 130.450 kb on - strandat 130.450 kb on - strandat 130.450 kb on - strandat 130.450 kb on - strandat 130.450 kb on - strandat 130.460 kb on - strandat 130.474 kb on - strandat 130.597 kb on + strandat 130.598 kb on - strandat 130.643 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
remove
126,629 + -1.1
127,162 - -0.4
127,291 - PP_0122 0.45 +0.4
127,291 - PP_0122 0.45 -0.4
127,312 - PP_0122 0.53 +0.1
127,327 + PP_0122 0.58 -0.6
127,328 - PP_0122 0.58 -0.2
127,424 + -0.5
127,425 - -0.0
127,450 + -0.3
127,455 + -0.1
127,455 + +0.2
127,455 + +0.1
127,456 - -1.3
128,462 + PP_0123 0.34 -0.5
128,463 - PP_0123 0.34 +0.2
128,463 - PP_0123 0.34 -0.3
128,618 + PP_0123 0.40 -1.6
128,618 + -0.1
128,619 - PP_0123 0.40 -0.3
128,619 - PP_0123 0.40 -0.3
128,619 - PP_0123 0.40 -2.4
128,639 + PP_0123 0.41 -1.2
128,742 - PP_0123 0.44 -0.3
128,819 + PP_0123 0.47 -1.6
128,819 + PP_0123 0.47 -0.7
128,819 + PP_0123 0.47 -2.1
128,820 - PP_0123 0.47 +0.0
128,820 - PP_0123 0.47 -0.5
128,873 + PP_0123 0.49 -1.5
128,932 + PP_0123 0.51 +0.0
128,932 + PP_0123 0.51 -1.0
128,933 - PP_0123 0.51 -1.3
128,970 - PP_0123 0.53 -0.3
129,141 + PP_0123 0.59 -1.1
129,191 + PP_0123 0.61 -0.3
129,192 - PP_0123 0.61 -0.7
129,371 + PP_0123 0.67 -0.6
129,372 - PP_0123 0.67 -1.2
129,401 + PP_0123 0.68 -0.2
129,401 + PP_0123 0.68 -0.7
129,401 + PP_0123 0.68 -0.3
129,402 - PP_0123 0.68 -0.3
129,414 + PP_0123 0.69 -0.1
129,414 + PP_0123 0.69 +0.0
129,415 - PP_0123 0.69 -1.0
129,458 + PP_0123 0.70 -0.5
129,458 + PP_0123 0.70 -0.2
129,578 + PP_0123 0.75 +0.4
129,599 + PP_0123 0.76 +1.2
129,599 + PP_0123 0.76 -0.1
129,599 + PP_0123 0.76 +1.5
129,600 - PP_0123 0.76 -0.8
129,619 + PP_0123 0.76 -0.2
129,620 - PP_0123 0.76 -0.6
129,620 - PP_0123 0.76 +0.3
129,779 + PP_0123 0.82 -1.7
129,779 + PP_0123 0.82 -0.6
129,780 - PP_0123 0.82 -0.2
129,842 + PP_0123 0.84 -0.1
129,842 + PP_0123 0.84 +0.3
129,842 + PP_0123 0.84 -0.8
129,842 + PP_0123 0.84 -0.0
129,843 - PP_0123 0.84 -0.1
129,843 - PP_0123 0.84 -0.7
129,843 - PP_0123 0.84 -0.9
129,844 + PP_0123 0.84 -0.5
129,845 - PP_0123 0.84 -1.1
129,845 - PP_0123 0.84 -1.0
129,845 - -0.5
129,845 - PP_0123 0.84 -0.0
129,854 + PP_0123 0.85 -0.7
129,884 + PP_0123 0.86 -0.5
129,885 - PP_0123 0.86 -0.4
129,885 - PP_0123 0.86 -0.8
129,953 + PP_0123 0.88 -0.9
129,954 - PP_0123 0.88 +0.3
130,071 + -0.9
130,075 + -0.6
130,075 + -0.4
130,076 - +1.1
130,146 + +0.4
130,147 - +0.0
130,291 - +0.6
130,304 + +0.2
130,305 - -0.4
130,345 + -0.2
130,345 + +0.0
130,346 - -0.0
130,346 - -0.6
130,370 - -0.3
130,390 + -0.1
130,391 - -0.2
130,391 - +0.7
130,415 + -0.5
130,449 + +0.2
130,449 + +0.1
130,449 + -0.5
130,450 - -0.1
130,450 - +0.1
130,450 - -0.5
130,450 - -0.2
130,450 - -0.2
130,450 - -0.3
130,450 - -0.2
130,450 - -0.3
130,460 - -0.0
130,474 - -2.0
130,597 + -0.4
130,598 - +0.0
130,643 - -0.1

Or see this region's nucleotide sequence