Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proS and hns are separated by 264 nucleotides hns and yjjB are separated by 202 nucleotides
OKGIIK_11245: proS - proline--tRNA ligase, at 2,410,141 to 2,411,838
proS
OKGIIK_11250: hns - histidine biosynthesis protein, at 2,412,103 to 2,412,426
hns
OKGIIK_11255: yjjB - Putative threonine/serine exporter, at 2,412,629 to 2,413,909
yjjB
Position (kb)
2412
2413 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2411.891 kb on + strand at 2411.892 kb on - strand at 2411.941 kb on + strand at 2411.941 kb on + strand at 2411.941 kb on + strand at 2411.942 kb on - strand at 2411.942 kb on - strand at 2411.944 kb on + strand at 2411.945 kb on - strand at 2411.945 kb on - strand at 2411.945 kb on - strand at 2411.945 kb on - strand at 2411.945 kb on - strand at 2411.954 kb on + strand at 2411.954 kb on + strand at 2411.955 kb on - strand at 2411.967 kb on - strand at 2411.967 kb on - strand at 2411.991 kb on - strand at 2411.996 kb on + strand at 2412.061 kb on + strand at 2412.074 kb on + strand at 2412.075 kb on - strand at 2412.075 kb on - strand at 2412.084 kb on + strand at 2412.084 kb on + strand at 2412.085 kb on - strand at 2412.085 kb on - strand at 2412.089 kb on - strand at 2412.244 kb on + strand, within hns at 2412.244 kb on + strand, within hns at 2412.245 kb on - strand, within hns at 2412.245 kb on - strand, within hns at 2412.245 kb on - strand, within hns at 2412.245 kb on - strand, within hns at 2412.258 kb on + strand, within hns at 2412.258 kb on + strand, within hns at 2412.259 kb on - strand, within hns at 2412.259 kb on - strand, within hns at 2412.259 kb on - strand, within hns at 2412.259 kb on - strand, within hns at 2412.259 kb on - strand, within hns at 2412.316 kb on + strand, within hns at 2412.316 kb on + strand, within hns at 2412.317 kb on - strand, within hns at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.488 kb on + strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.489 kb on - strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.607 kb on + strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.608 kb on - strand at 2412.630 kb on + strand at 2412.630 kb on + strand at 2412.631 kb on - strand at 2412.631 kb on - strand at 2412.736 kb on + strand at 2412.736 kb on + strand at 2412.736 kb on + strand at 2412.736 kb on + strand at 2412.737 kb on - strand at 2412.948 kb on + strand, within yjjB at 2412.948 kb on + strand, within yjjB at 2412.948 kb on + strand, within yjjB at 2412.949 kb on - strand, within yjjB at 2412.949 kb on - strand, within yjjB at 2413.135 kb on - strand, within yjjB at 2413.268 kb on + strand, within yjjB at 2413.269 kb on - strand, within yjjB at 2413.269 kb on - strand, within yjjB at 2413.385 kb on + strand, within yjjB at 2413.386 kb on - strand, within yjjB at 2413.386 kb on - strand, within yjjB at 2413.386 kb on - strand, within yjjB
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 2,411,891 + -1.9 2,411,892 - -0.8 2,411,941 + -1.4 2,411,941 + -0.2 2,411,941 + -0.4 2,411,942 - -1.1 2,411,942 - +0.6 2,411,944 + -0.4 2,411,945 - +0.3 2,411,945 - -3.3 2,411,945 - -0.4 2,411,945 - +0.6 2,411,945 - -2.4 2,411,954 + -0.2 2,411,954 + -1.2 2,411,955 - +1.6 2,411,967 - -1.2 2,411,967 - +0.3 2,411,991 - -1.8 2,411,996 + -0.0 2,412,061 + -0.4 2,412,074 + -1.3 2,412,075 - -0.0 2,412,075 - -1.8 2,412,084 + -1.4 2,412,084 + -1.9 2,412,085 - -1.8 2,412,085 - -2.8 2,412,089 - -1.8 2,412,244 + hns OKGIIK_11250 0.44 -2.7 2,412,244 + hns OKGIIK_11250 0.44 -1.2 2,412,245 - hns OKGIIK_11250 0.44 -3.0 2,412,245 - hns OKGIIK_11250 0.44 -0.0 2,412,245 - hns OKGIIK_11250 0.44 +0.6 2,412,245 - hns OKGIIK_11250 0.44 +1.6 2,412,258 + hns OKGIIK_11250 0.48 -0.0 2,412,258 + hns OKGIIK_11250 0.48 +1.6 2,412,259 - hns OKGIIK_11250 0.48 -0.8 2,412,259 - hns OKGIIK_11250 0.48 -4.7 2,412,259 - hns OKGIIK_11250 0.48 -0.4 2,412,259 - hns OKGIIK_11250 0.48 -1.8 2,412,259 - hns OKGIIK_11250 0.48 -1.6 2,412,316 + hns OKGIIK_11250 0.66 -3.8 2,412,316 + hns OKGIIK_11250 0.66 -0.4 2,412,317 - hns OKGIIK_11250 0.66 +1.6 2,412,488 + -1.0 2,412,488 + -2.8 2,412,488 + -1.3 2,412,488 + -1.0 2,412,488 + -2.4 2,412,488 + -1.8 2,412,488 + -2.0 2,412,488 + -2.1 2,412,488 + -1.0 2,412,488 + -2.0 2,412,488 + -1.4 2,412,488 + -2.0 2,412,488 + -2.1 2,412,488 + -0.9 2,412,488 + +1.0 2,412,488 + -1.2 2,412,488 + -1.1 2,412,488 + -2.0 2,412,488 + -0.9 2,412,488 + -2.5 2,412,489 - +0.7 2,412,489 - -1.4 2,412,489 - -0.7 2,412,489 - -2.2 2,412,489 - +0.2 2,412,489 - -0.5 2,412,489 - +1.3 2,412,489 - -0.7 2,412,489 - -0.4 2,412,489 - -0.4 2,412,489 - -1.4 2,412,489 - -1.6 2,412,489 - -0.6 2,412,489 - -0.8 2,412,489 - +0.4 2,412,489 - +0.3 2,412,489 - -0.6 2,412,489 - -3.3 2,412,489 - -0.8 2,412,489 - +0.3 2,412,489 - +1.3 2,412,489 - -0.4 2,412,489 - -0.7 2,412,489 - +0.6 2,412,489 - -2.0 2,412,607 + -1.0 2,412,607 + -0.8 2,412,607 + -0.4 2,412,607 + -0.7 2,412,607 + -1.0 2,412,607 + -0.8 2,412,607 + -1.6 2,412,607 + -1.0 2,412,607 + -0.8 2,412,607 + -2.0 2,412,607 + -1.4 2,412,607 + -1.4 2,412,607 + -2.1 2,412,607 + -2.6 2,412,607 + +0.6 2,412,607 + -1.5 2,412,607 + -1.6 2,412,607 + -0.3 2,412,607 + -0.4 2,412,607 + -1.1 2,412,607 + -1.6 2,412,607 + -0.8 2,412,607 + -0.8 2,412,607 + -1.0 2,412,607 + +1.0 2,412,607 + +0.4 2,412,607 + -2.7 2,412,607 + -1.8 2,412,608 - -1.4 2,412,608 - -1.2 2,412,608 - -0.4 2,412,608 - +1.0 2,412,608 - -0.7 2,412,608 - -0.0 2,412,608 - -0.0 2,412,608 - -0.0 2,412,608 - +0.5 2,412,608 - -2.1 2,412,608 - -0.4 2,412,608 - -1.9 2,412,608 - -0.4 2,412,608 - -0.0 2,412,608 - -2.2 2,412,608 - -1.4 2,412,608 - -2.0 2,412,608 - -0.1 2,412,608 - +0.8 2,412,608 - -2.1 2,412,608 - -1.4 2,412,608 - -0.5 2,412,608 - -1.9 2,412,608 - -1.0 2,412,608 - -2.5 2,412,608 - -1.3 2,412,608 - -2.1 2,412,608 - -0.0 2,412,608 - -1.0 2,412,608 - -1.2 2,412,608 - -0.2 2,412,608 - -0.8 2,412,608 - -1.8 2,412,608 - -1.1 2,412,608 - -2.0 2,412,608 - -2.7 2,412,608 - -1.6 2,412,630 + +0.1 2,412,630 + -0.8 2,412,631 - -1.6 2,412,631 - +0.6 2,412,736 + +0.3 2,412,736 + +0.2 2,412,736 + -1.3 2,412,736 + -1.2 2,412,737 - -0.1 2,412,948 + yjjB OKGIIK_11255 0.25 -3.1 2,412,948 + yjjB OKGIIK_11255 0.25 -2.0 2,412,948 + yjjB OKGIIK_11255 0.25 -1.9 2,412,949 - yjjB OKGIIK_11255 0.25 +0.6 2,412,949 - yjjB OKGIIK_11255 0.25 -0.0 2,413,135 - yjjB OKGIIK_11255 0.40 -1.8 2,413,268 + yjjB OKGIIK_11255 0.50 -0.4 2,413,269 - yjjB OKGIIK_11255 0.50 -1.0 2,413,269 - yjjB OKGIIK_11255 0.50 -1.6 2,413,385 + yjjB OKGIIK_11255 0.59 -1.6 2,413,386 - yjjB OKGIIK_11255 0.59 -2.1 2,413,386 - yjjB OKGIIK_11255 0.59 -1.0 2,413,386 - yjjB OKGIIK_11255 0.59 -0.4
Or see this region's nucleotide sequence