Strain Fitness in Escherichia coli ECRC98 around JDDGAC_04225

Experiment: WM_TP6

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthrpA and azoR are separated by 200 nucleotidesazoR and ynbD are separated by 50 nucleotides JDDGAC_04220: hrpA - ATP-dependent RNA helicase HrpA, at 736,437 to 740,339 hrpA JDDGAC_04225: azoR - FMN-dependent NADH-azoreductase, at 740,540 to 741,145 azoR JDDGAC_04230: ynbD - Uncharacterized protein YnbD, at 741,196 to 742,512 ynbD Position (kb) 740 741 742Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 739.825 kb on - strand, within hrpAat 739.842 kb on - strand, within hrpAat 739.848 kb on + strand, within hrpAat 739.849 kb on - strand, within hrpAat 739.849 kb on - strand, within hrpAat 740.050 kb on - strandat 740.345 kb on + strandat 740.391 kb on - strandat 740.392 kb on + strandat 740.393 kb on - strandat 740.523 kb on - strandat 740.526 kb on + strandat 740.890 kb on + strand, within azoRat 740.950 kb on + strand, within azoRat 740.953 kb on + strand, within azoRat 741.005 kb on + strand, within azoRat 741.005 kb on + strand, within azoRat 741.029 kb on - strand, within azoRat 741.171 kb on + strandat 741.172 kb on - strandat 741.249 kb on + strandat 741.250 kb on - strandat 741.250 kb on - strandat 741.298 kb on - strandat 741.313 kb on + strandat 741.395 kb on - strand, within ynbDat 741.547 kb on - strand, within ynbDat 741.570 kb on - strand, within ynbDat 741.624 kb on + strand, within ynbDat 741.628 kb on + strand, within ynbDat 741.770 kb on - strand, within ynbDat 741.787 kb on + strand, within ynbDat 741.788 kb on - strand, within ynbDat 741.846 kb on - strand, within ynbDat 741.846 kb on - strand, within ynbDat 741.931 kb on - strand, within ynbDat 741.961 kb on - strand, within ynbDat 742.069 kb on + strand, within ynbDat 742.110 kb on - strand, within ynbD

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP6
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739,825 - hrpA JDDGAC_04220 0.87 +0.9
739,842 - hrpA JDDGAC_04220 0.87 +0.4
739,848 + hrpA JDDGAC_04220 0.87 +1.6
739,849 - hrpA JDDGAC_04220 0.87 +1.9
739,849 - hrpA JDDGAC_04220 0.87 -2.8
740,050 - -0.6
740,345 + +2.2
740,391 - +1.0
740,392 + -0.2
740,393 - +0.5
740,523 - -1.6
740,526 + +1.0
740,890 + azoR JDDGAC_04225 0.58 -0.8
740,950 + azoR JDDGAC_04225 0.68 -0.8
740,953 + azoR JDDGAC_04225 0.68 -1.6
741,005 + azoR JDDGAC_04225 0.77 +1.4
741,005 + azoR JDDGAC_04225 0.77 -1.8
741,029 - azoR JDDGAC_04225 0.81 -2.4
741,171 + +0.3
741,172 - -1.9
741,249 + -2.2
741,250 - +0.8
741,250 - +0.5
741,298 - +0.5
741,313 + -0.3
741,395 - ynbD JDDGAC_04230 0.15 -0.5
741,547 - ynbD JDDGAC_04230 0.27 +0.3
741,570 - ynbD JDDGAC_04230 0.28 -0.1
741,624 + ynbD JDDGAC_04230 0.32 +1.6
741,628 + ynbD JDDGAC_04230 0.33 -0.6
741,770 - ynbD JDDGAC_04230 0.44 +0.7
741,787 + ynbD JDDGAC_04230 0.45 -0.2
741,788 - ynbD JDDGAC_04230 0.45 -1.0
741,846 - ynbD JDDGAC_04230 0.49 +0.2
741,846 - ynbD JDDGAC_04230 0.49 +0.1
741,931 - ynbD JDDGAC_04230 0.56 -0.5
741,961 - ynbD JDDGAC_04230 0.58 +1.4
742,069 + ynbD JDDGAC_04230 0.66 +1.1
742,110 - ynbD JDDGAC_04230 0.69 +0.9

Or see this region's nucleotide sequence