Experiment: NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15345 and EX31_RS15350 overlap by 14 nucleotides EX31_RS15350 and EX31_RS15355 are separated by 154 nucleotides EX31_RS15355 and EX31_RS15360 are separated by 17 nucleotides EX31_RS15360 and EX31_RS15365 are separated by 46 nucleotides
EX31_RS15345: EX31_RS15345 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifN, at 20,494 to 21,879
_RS15345
EX31_RS15350: EX31_RS15350 - nitrogen fixation protein NifX, at 21,866 to 22,330
_RS15350
EX31_RS15355: EX31_RS15355 - hypothetical protein, at 22,485 to 22,823
_RS15355
EX31_RS15360: EX31_RS15360 - Fe-S cluster assembly protein NifU, at 22,841 to 23,668
_RS15360
EX31_RS15365: EX31_RS15365 - cysteine desulfurase NifS, at 23,715 to 24,920
_RS15365
Position (kb) 22
23
24 Strain fitness (log2 ratio) -2
-1
0
1
2 at 21.992 kb on + strand, within EX31_RS15350 at 22.112 kb on + strand, within EX31_RS15350 at 22.112 kb on + strand, within EX31_RS15350 at 22.112 kb on + strand, within EX31_RS15350 at 22.113 kb on - strand, within EX31_RS15350 at 22.113 kb on - strand, within EX31_RS15350 at 22.113 kb on - strand, within EX31_RS15350 at 22.113 kb on - strand, within EX31_RS15350 at 22.345 kb on + strand at 22.346 kb on - strand at 22.364 kb on - strand at 22.405 kb on + strand at 22.405 kb on + strand at 22.406 kb on - strand at 22.419 kb on - strand at 22.461 kb on + strand at 22.461 kb on + strand at 22.462 kb on - strand at 22.462 kb on - strand at 22.493 kb on - strand at 22.573 kb on + strand, within EX31_RS15355 at 22.573 kb on + strand, within EX31_RS15355 at 22.573 kb on + strand, within EX31_RS15355 at 22.574 kb on - strand, within EX31_RS15355 at 22.574 kb on - strand, within EX31_RS15355 at 22.645 kb on + strand, within EX31_RS15355 at 22.645 kb on + strand, within EX31_RS15355 at 22.646 kb on - strand, within EX31_RS15355 at 22.646 kb on - strand, within EX31_RS15355 at 22.696 kb on + strand, within EX31_RS15355 at 22.696 kb on + strand, within EX31_RS15355 at 22.700 kb on - strand, within EX31_RS15355 at 22.700 kb on - strand, within EX31_RS15355 at 22.700 kb on - strand, within EX31_RS15355 at 22.700 kb on - strand, within EX31_RS15355 at 22.747 kb on + strand, within EX31_RS15355 at 22.748 kb on - strand, within EX31_RS15355 at 22.799 kb on + strand at 22.799 kb on + strand at 22.799 kb on + strand at 22.799 kb on + strand at 22.799 kb on + strand at 22.799 kb on + strand at 22.800 kb on - strand at 22.800 kb on - strand at 22.835 kb on - strand at 22.850 kb on + strand at 23.198 kb on + strand, within EX31_RS15360 at 23.198 kb on + strand, within EX31_RS15360 at 23.198 kb on + strand, within EX31_RS15360 at 23.199 kb on - strand, within EX31_RS15360 at 23.199 kb on - strand, within EX31_RS15360 at 23.199 kb on - strand, within EX31_RS15360 at 23.201 kb on + strand, within EX31_RS15360 at 23.202 kb on - strand, within EX31_RS15360 at 23.245 kb on + strand, within EX31_RS15360 at 23.333 kb on + strand, within EX31_RS15360 at 23.333 kb on + strand, within EX31_RS15360 at 23.333 kb on + strand, within EX31_RS15360 at 23.334 kb on - strand, within EX31_RS15360 at 23.632 kb on + strand at 23.633 kb on - strand at 23.633 kb on - strand at 23.642 kb on + strand at 23.642 kb on + strand at 23.642 kb on + strand at 23.642 kb on + strand at 23.642 kb on + strand at 23.642 kb on + strand at 23.642 kb on + strand at 23.642 kb on + strand at 23.642 kb on + strand at 23.643 kb on - strand at 23.643 kb on - strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.645 kb on - strand at 23.645 kb on - strand at 23.645 kb on - strand at 23.645 kb on - strand at 23.645 kb on - strand at 23.700 kb on - strand at 23.727 kb on + strand at 23.727 kb on + strand at 23.727 kb on + strand at 23.727 kb on + strand at 23.787 kb on + strand at 23.799 kb on + strand at 23.898 kb on + strand, within EX31_RS15365 at 23.898 kb on + strand, within EX31_RS15365 at 23.898 kb on + strand, within EX31_RS15365 at 23.899 kb on - strand, within EX31_RS15365 at 23.899 kb on - strand, within EX31_RS15365 at 23.899 kb on - strand, within EX31_RS15365 at 23.900 kb on + strand, within EX31_RS15365 at 23.901 kb on - strand, within EX31_RS15365 at 23.901 kb on - strand, within EX31_RS15365 at 23.913 kb on - strand, within EX31_RS15365 at 23.955 kb on + strand, within EX31_RS15365 at 23.955 kb on + strand, within EX31_RS15365 at 23.956 kb on - strand, within EX31_RS15365 at 23.993 kb on + strand, within EX31_RS15365 at 24.057 kb on + strand, within EX31_RS15365 at 24.057 kb on + strand, within EX31_RS15365 at 24.057 kb on + strand, within EX31_RS15365 at 24.057 kb on + strand, within EX31_RS15365 at 24.057 kb on + strand, within EX31_RS15365 at 24.057 kb on + strand, within EX31_RS15365 at 24.057 kb on + strand, within EX31_RS15365 at 24.058 kb on - strand, within EX31_RS15365 at 24.058 kb on - strand, within EX31_RS15365 at 24.058 kb on - strand, within EX31_RS15365 at 24.058 kb on - strand, within EX31_RS15365 at 24.072 kb on - strand, within EX31_RS15365 at 24.111 kb on + strand, within EX31_RS15365 at 24.111 kb on + strand, within EX31_RS15365 at 24.112 kb on - strand, within EX31_RS15365 at 24.112 kb on - strand, within EX31_RS15365 at 24.112 kb on - strand, within EX31_RS15365 at 24.112 kb on - strand, within EX31_RS15365 at 24.114 kb on + strand, within EX31_RS15365 at 24.115 kb on - strand, within EX31_RS15365 at 24.115 kb on - strand, within EX31_RS15365 at 24.115 kb on - strand, within EX31_RS15365 at 24.181 kb on + strand, within EX31_RS15365 at 24.181 kb on + strand, within EX31_RS15365 at 24.182 kb on - strand, within EX31_RS15365 at 24.182 kb on - strand, within EX31_RS15365 at 24.317 kb on + strand, within EX31_RS15365 at 24.317 kb on + strand, within EX31_RS15365 at 24.318 kb on - strand, within EX31_RS15365 at 24.351 kb on + strand, within EX31_RS15365 at 24.352 kb on - strand, within EX31_RS15365 at 24.352 kb on - strand, within EX31_RS15365 at 24.482 kb on + strand, within EX31_RS15365 at 24.482 kb on + strand, within EX31_RS15365 at 24.482 kb on + strand, within EX31_RS15365 at 24.482 kb on + strand, within EX31_RS15365 at 24.482 kb on + strand, within EX31_RS15365 at 24.482 kb on + strand, within EX31_RS15365 at 24.482 kb on + strand, within EX31_RS15365 at 24.483 kb on - strand, within EX31_RS15365 at 24.483 kb on - strand, within EX31_RS15365 at 24.483 kb on - strand, within EX31_RS15365 at 24.483 kb on - strand, within EX31_RS15365 at 24.483 kb on - strand, within EX31_RS15365 at 24.483 kb on - strand, within EX31_RS15365 at 24.593 kb on + strand, within EX31_RS15365 at 24.593 kb on + strand, within EX31_RS15365 at 24.593 kb on + strand, within EX31_RS15365 at 24.594 kb on - strand, within EX31_RS15365 at 24.594 kb on - strand, within EX31_RS15365 at 24.615 kb on + strand, within EX31_RS15365 at 24.615 kb on + strand, within EX31_RS15365 at 24.615 kb on + strand, within EX31_RS15365 at 24.615 kb on + strand, within EX31_RS15365 at 24.615 kb on + strand, within EX31_RS15365 at 24.615 kb on + strand, within EX31_RS15365 at 24.615 kb on + strand, within EX31_RS15365 at 24.615 kb on + strand, within EX31_RS15365 at 24.616 kb on - strand, within EX31_RS15365 at 24.616 kb on - strand, within EX31_RS15365 at 24.616 kb on - strand, within EX31_RS15365 at 24.616 kb on - strand, within EX31_RS15365 at 24.616 kb on - strand, within EX31_RS15365 at 24.617 kb on + strand, within EX31_RS15365 at 24.617 kb on + strand, within EX31_RS15365 at 24.618 kb on - strand, within EX31_RS15365 at 24.618 kb on - strand, within EX31_RS15365 at 24.647 kb on + strand, within EX31_RS15365 at 24.648 kb on - strand, within EX31_RS15365 at 24.659 kb on + strand, within EX31_RS15365
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum remove 21,992 + EX31_RS15350 0.27 -0.0 22,112 + EX31_RS15350 0.53 -0.1 22,112 + EX31_RS15350 0.53 -0.3 22,112 + EX31_RS15350 0.53 -0.3 22,113 - EX31_RS15350 0.53 -2.7 22,113 - EX31_RS15350 0.53 +1.2 22,113 - EX31_RS15350 0.53 +0.9 22,113 - EX31_RS15350 0.53 -1.6 22,345 + -0.2 22,346 - +0.1 22,364 - -0.4 22,405 + -0.3 22,405 + +0.1 22,406 - +0.2 22,419 - +0.7 22,461 + +1.0 22,461 + -0.5 22,462 - +0.5 22,462 - +1.1 22,493 - -0.4 22,573 + EX31_RS15355 0.26 +0.3 22,573 + EX31_RS15355 0.26 -0.5 22,573 + EX31_RS15355 0.26 -0.4 22,574 - EX31_RS15355 0.26 -0.6 22,574 - EX31_RS15355 0.26 +0.1 22,645 + EX31_RS15355 0.47 -0.1 22,645 + EX31_RS15355 0.47 -1.0 22,646 - EX31_RS15355 0.47 +0.9 22,646 - EX31_RS15355 0.47 +0.9 22,696 + EX31_RS15355 0.62 -0.6 22,696 + EX31_RS15355 0.62 +0.2 22,700 - EX31_RS15355 0.63 +0.7 22,700 - EX31_RS15355 0.63 +0.3 22,700 - EX31_RS15355 0.63 -1.0 22,700 - EX31_RS15355 0.63 +0.8 22,747 + EX31_RS15355 0.77 -0.3 22,748 - EX31_RS15355 0.78 +0.7 22,799 + +0.0 22,799 + -0.4 22,799 + -0.7 22,799 + +0.1 22,799 + -0.0 22,799 + -1.7 22,800 - +0.4 22,800 - +0.5 22,835 - -0.3 22,850 + -0.0 23,198 + EX31_RS15360 0.43 +0.7 23,198 + EX31_RS15360 0.43 -0.1 23,198 + EX31_RS15360 0.43 +0.7 23,199 - EX31_RS15360 0.43 +0.6 23,199 - EX31_RS15360 0.43 +0.8 23,199 - EX31_RS15360 0.43 -1.0 23,201 + EX31_RS15360 0.43 -0.1 23,202 - EX31_RS15360 0.44 +0.5 23,245 + EX31_RS15360 0.49 +1.0 23,333 + EX31_RS15360 0.59 +0.3 23,333 + EX31_RS15360 0.59 +0.7 23,333 + EX31_RS15360 0.59 +0.7 23,334 - EX31_RS15360 0.60 +1.9 23,632 + -1.1 23,633 - -0.1 23,633 - -0.8 23,642 + +0.1 23,642 + -0.2 23,642 + -0.7 23,642 + +0.2 23,642 + -0.7 23,642 + -0.5 23,642 + +0.2 23,642 + -0.1 23,642 + +1.2 23,643 - -0.2 23,643 - -0.6 23,644 + -0.2 23,644 + +1.4 23,644 + -0.3 23,644 + -0.2 23,644 + -1.3 23,644 + +0.2 23,644 + +0.7 23,644 + -0.6 23,644 + +1.2 23,644 + -1.6 23,644 + -0.7 23,644 + -0.9 23,644 + -0.8 23,645 - -1.4 23,645 - -2.0 23,645 - -1.9 23,645 - -2.0 23,645 - +0.6 23,700 - -1.5 23,727 + +0.0 23,727 + -1.3 23,727 + -1.3 23,727 + -1.0 23,787 + +0.2 23,799 + +0.4 23,898 + EX31_RS15365 0.15 -0.8 23,898 + EX31_RS15365 0.15 -0.1 23,898 + EX31_RS15365 0.15 -0.2 23,899 - EX31_RS15365 0.15 +0.6 23,899 - EX31_RS15365 0.15 +1.0 23,899 - EX31_RS15365 0.15 +0.4 23,900 + EX31_RS15365 0.15 +0.2 23,901 - EX31_RS15365 0.15 -1.0 23,901 - EX31_RS15365 0.15 +0.3 23,913 - EX31_RS15365 0.16 +0.1 23,955 + EX31_RS15365 0.20 -1.0 23,955 + EX31_RS15365 0.20 +0.7 23,956 - EX31_RS15365 0.20 +0.1 23,993 + EX31_RS15365 0.23 -0.0 24,057 + EX31_RS15365 0.28 +0.0 24,057 + EX31_RS15365 0.28 -0.3 24,057 + EX31_RS15365 0.28 -0.8 24,057 + EX31_RS15365 0.28 -0.3 24,057 + EX31_RS15365 0.28 -1.1 24,057 + EX31_RS15365 0.28 +0.7 24,057 + EX31_RS15365 0.28 -0.1 24,058 - EX31_RS15365 0.28 -0.1 24,058 - EX31_RS15365 0.28 +0.7 24,058 - EX31_RS15365 0.28 +0.0 24,058 - EX31_RS15365 0.28 -0.2 24,072 - EX31_RS15365 0.30 +0.3 24,111 + EX31_RS15365 0.33 +1.3 24,111 + EX31_RS15365 0.33 -0.5 24,112 - EX31_RS15365 0.33 +0.5 24,112 - EX31_RS15365 0.33 -2.4 24,112 - EX31_RS15365 0.33 +0.5 24,112 - EX31_RS15365 0.33 +1.3 24,114 + EX31_RS15365 0.33 -0.2 24,115 - EX31_RS15365 0.33 -1.3 24,115 - EX31_RS15365 0.33 -0.4 24,115 - EX31_RS15365 0.33 +0.6 24,181 + EX31_RS15365 0.39 +0.3 24,181 + EX31_RS15365 0.39 +0.1 24,182 - EX31_RS15365 0.39 -0.2 24,182 - EX31_RS15365 0.39 -0.8 24,317 + EX31_RS15365 0.50 -1.0 24,317 + EX31_RS15365 0.50 +0.2 24,318 - EX31_RS15365 0.50 -0.0 24,351 + EX31_RS15365 0.53 -0.1 24,352 - EX31_RS15365 0.53 +1.3 24,352 - EX31_RS15365 0.53 -0.4 24,482 + EX31_RS15365 0.64 -0.0 24,482 + EX31_RS15365 0.64 +0.0 24,482 + EX31_RS15365 0.64 -0.1 24,482 + EX31_RS15365 0.64 -1.1 24,482 + EX31_RS15365 0.64 +0.5 24,482 + EX31_RS15365 0.64 -0.2 24,482 + EX31_RS15365 0.64 -1.6 24,483 - EX31_RS15365 0.64 +0.1 24,483 - EX31_RS15365 0.64 -0.8 24,483 - EX31_RS15365 0.64 -1.8 24,483 - EX31_RS15365 0.64 -0.6 24,483 - EX31_RS15365 0.64 -1.7 24,483 - EX31_RS15365 0.64 -1.9 24,593 + EX31_RS15365 0.73 +0.6 24,593 + EX31_RS15365 0.73 -2.2 24,593 + EX31_RS15365 0.73 +0.2 24,594 - EX31_RS15365 0.73 +0.3 24,594 - EX31_RS15365 0.73 -0.4 24,615 + EX31_RS15365 0.75 -0.1 24,615 + EX31_RS15365 0.75 +0.1 24,615 + EX31_RS15365 0.75 -0.7 24,615 + EX31_RS15365 0.75 +1.4 24,615 + EX31_RS15365 0.75 -1.3 24,615 + EX31_RS15365 0.75 +0.3 24,615 + EX31_RS15365 0.75 +0.1 24,615 + EX31_RS15365 0.75 -0.0 24,616 - EX31_RS15365 0.75 -0.6 24,616 - EX31_RS15365 0.75 +0.5 24,616 - EX31_RS15365 0.75 -0.0 24,616 - EX31_RS15365 0.75 +1.1 24,616 - EX31_RS15365 0.75 +0.2 24,617 + EX31_RS15365 0.75 +0.5 24,617 + EX31_RS15365 0.75 -0.2 24,618 - EX31_RS15365 0.75 -0.6 24,618 - EX31_RS15365 0.75 -0.3 24,647 + EX31_RS15365 0.77 -1.6 24,648 - EX31_RS15365 0.77 -0.1 24,659 + EX31_RS15365 0.78 -1.1
Or see this region's nucleotide sequence