Strain Fitness in Rahnella sp. WP5 around EX31_RS00985

Experiment: MGL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS00975 and EX31_RS00980 are separated by 13 nucleotidesEX31_RS00980 and EX31_RS00985 overlap by 1 nucleotidesEX31_RS00985 and EX31_RS00990 are separated by 11 nucleotides EX31_RS00975: EX31_RS00975 - type VI secretion system tip protein VgrG, at 211,176 to 213,089 _RS00975 EX31_RS00980: EX31_RS00980 - hypothetical protein, at 213,103 to 213,618 _RS00980 EX31_RS00985: EX31_RS00985 - type VI secretion protein, at 213,618 to 213,917 _RS00985 EX31_RS00990: EX31_RS00990 - type VI secretion system protein TssA, at 213,929 to 214,993 _RS00990 Position (kb) 213 214Strain fitness (log2 ratio) -2 -1 0 1at 212.702 kb on + strand, within EX31_RS00975at 212.702 kb on + strand, within EX31_RS00975at 212.867 kb on + strand, within EX31_RS00975at 212.924 kb on - strandat 212.967 kb on - strandat 213.017 kb on + strandat 213.017 kb on + strandat 213.116 kb on + strandat 213.116 kb on + strandat 213.142 kb on + strandat 213.142 kb on + strandat 213.142 kb on + strandat 213.564 kb on + strand, within EX31_RS00980at 213.564 kb on + strand, within EX31_RS00980at 213.564 kb on + strand, within EX31_RS00980at 213.564 kb on + strand, within EX31_RS00980at 213.565 kb on - strand, within EX31_RS00980at 213.613 kb on + strandat 213.647 kb on + strandat 213.733 kb on + strand, within EX31_RS00985at 213.734 kb on - strand, within EX31_RS00985at 213.737 kb on + strand, within EX31_RS00985at 213.746 kb on + strand, within EX31_RS00985at 213.746 kb on + strand, within EX31_RS00985at 213.905 kb on - strandat 213.915 kb on + strandat 213.916 kb on - strandat 213.922 kb on + strandat 213.923 kb on - strandat 213.991 kb on + strandat 213.998 kb on + strandat 213.998 kb on + strandat 213.998 kb on + strandat 213.998 kb on + strandat 214.010 kb on + strandat 214.010 kb on + strandat 214.010 kb on + strandat 214.050 kb on - strand, within EX31_RS00990at 214.050 kb on - strand, within EX31_RS00990at 214.081 kb on - strand, within EX31_RS00990at 214.477 kb on + strand, within EX31_RS00990at 214.555 kb on + strand, within EX31_RS00990at 214.555 kb on + strand, within EX31_RS00990at 214.555 kb on + strand, within EX31_RS00990at 214.556 kb on - strand, within EX31_RS00990at 214.580 kb on + strand, within EX31_RS00990at 214.581 kb on - strand, within EX31_RS00990at 214.630 kb on + strand, within EX31_RS00990at 214.716 kb on + strand, within EX31_RS00990at 214.716 kb on + strand, within EX31_RS00990at 214.717 kb on - strand, within EX31_RS00990at 214.717 kb on - strand, within EX31_RS00990at 214.717 kb on - strand, within EX31_RS00990at 214.717 kb on - strand, within EX31_RS00990at 214.720 kb on - strand, within EX31_RS00990at 214.720 kb on - strand, within EX31_RS00990at 214.768 kb on + strand, within EX31_RS00990at 214.768 kb on + strand, within EX31_RS00990at 214.768 kb on + strand, within EX31_RS00990at 214.900 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction MGL
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212,702 + EX31_RS00975 0.80 +0.4
212,702 + EX31_RS00975 0.80 -0.2
212,867 + EX31_RS00975 0.88 +0.9
212,924 - -0.6
212,967 - +0.7
213,017 + -0.1
213,017 + +1.1
213,116 + +0.6
213,116 + -1.6
213,142 + +1.5
213,142 + +0.6
213,142 + -0.7
213,564 + EX31_RS00980 0.89 +0.4
213,564 + EX31_RS00980 0.89 +1.7
213,564 + EX31_RS00980 0.89 +0.8
213,564 + EX31_RS00980 0.89 +1.2
213,565 - EX31_RS00980 0.90 -0.5
213,613 + -0.6
213,647 + -0.3
213,733 + EX31_RS00985 0.38 -0.8
213,734 - EX31_RS00985 0.39 -0.3
213,737 + EX31_RS00985 0.40 +0.4
213,746 + EX31_RS00985 0.43 -1.4
213,746 + EX31_RS00985 0.43 -1.6
213,905 - -0.1
213,915 + +0.3
213,916 - -0.8
213,922 + +0.5
213,923 - -1.4
213,991 + +0.2
213,998 + -0.2
213,998 + +0.4
213,998 + +0.1
213,998 + -1.7
214,010 + -0.0
214,010 + +0.9
214,010 + +0.1
214,050 - EX31_RS00990 0.11 +0.1
214,050 - EX31_RS00990 0.11 -0.2
214,081 - EX31_RS00990 0.14 -1.2
214,477 + EX31_RS00990 0.51 +0.7
214,555 + EX31_RS00990 0.59 -1.0
214,555 + EX31_RS00990 0.59 -1.3
214,555 + EX31_RS00990 0.59 -1.3
214,556 - EX31_RS00990 0.59 -2.3
214,580 + EX31_RS00990 0.61 -0.2
214,581 - EX31_RS00990 0.61 -1.9
214,630 + EX31_RS00990 0.66 -0.0
214,716 + EX31_RS00990 0.74 +0.5
214,716 + EX31_RS00990 0.74 -2.4
214,717 - EX31_RS00990 0.74 -0.2
214,717 - EX31_RS00990 0.74 -1.3
214,717 - EX31_RS00990 0.74 +0.7
214,717 - EX31_RS00990 0.74 +0.2
214,720 - EX31_RS00990 0.74 -1.3
214,720 - EX31_RS00990 0.74 -0.1
214,768 + EX31_RS00990 0.79 +0.1
214,768 + EX31_RS00990 0.79 +1.6
214,768 + EX31_RS00990 0.79 -1.2
214,900 + +0.3

Or see this region's nucleotide sequence