Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03820 and PS417_03825 overlap by 4 nucleotides PS417_03825 and PS417_03830 are separated by 130 nucleotides PS417_03830 and PS417_03835 are separated by 300 nucleotides PS417_03835 and PS417_03840 are separated by 10 nucleotides PS417_03840 and PS417_03845 are separated by 5 nucleotides PS417_03845 and PS417_03850 are separated by 94 nucleotides PS417_03850 and PS417_03855 are separated by 192 nucleotides
PS417_03820: PS417_03820 - ribosomal large subunit pseudouridine synthase D, at 861,582 to 862,544
_03820
PS417_03825: PS417_03825 - laccase, at 862,541 to 863,266
_03825
PS417_03830: PS417_03830 - protein disaggregation chaperone, at 863,397 to 865,961
_03830
PS417_03835: PS417_03835 - tRNA-Asn, at 866,262 to 866,337
_03835
PS417_03840: PS417_03840 - tRNA-Pro, at 866,348 to 866,424
_03840
PS417_03845: PS417_03845 - tRNA-Lys, at 866,430 to 866,505
_03845
PS417_03850: PS417_03850 - tRNA-Pro, at 866,600 to 866,676
_03850
PS417_03855: PS417_03855 - NADH dehydrogenase, at 866,869 to 868,167
_03855
Position (kb) 863
864
865
866 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 862.717 kb on + strand, within PS417_03825 at 862.718 kb on - strand, within PS417_03825 at 862.974 kb on + strand, within PS417_03825 at 862.974 kb on + strand, within PS417_03825 at 862.974 kb on + strand, within PS417_03825 at 862.975 kb on - strand, within PS417_03825 at 863.117 kb on + strand, within PS417_03825 at 863.117 kb on + strand, within PS417_03825 at 863.118 kb on - strand, within PS417_03825 at 863.118 kb on - strand, within PS417_03825 at 863.118 kb on - strand, within PS417_03825 at 863.118 kb on - strand, within PS417_03825 at 863.118 kb on - strand, within PS417_03825 at 863.118 kb on - strand, within PS417_03825 at 863.119 kb on + strand, within PS417_03825 at 863.119 kb on + strand, within PS417_03825 at 863.119 kb on + strand, within PS417_03825 at 863.120 kb on - strand, within PS417_03825 at 863.248 kb on + strand at 863.249 kb on - strand at 863.324 kb on + strand at 863.324 kb on + strand at 863.325 kb on - strand at 863.325 kb on - strand at 863.325 kb on - strand at 863.325 kb on - strand at 863.332 kb on + strand at 863.363 kb on + strand at 863.364 kb on - strand at 863.364 kb on - strand at 863.366 kb on - strand at 863.396 kb on - strand at 863.402 kb on + strand at 863.426 kb on - strand at 863.531 kb on + strand at 863.642 kb on + strand at 863.642 kb on + strand at 863.642 kb on + strand at 863.642 kb on + strand at 863.643 kb on - strand at 863.643 kb on - strand at 863.643 kb on - strand at 863.882 kb on + strand, within PS417_03830 at 863.886 kb on + strand, within PS417_03830 at 863.886 kb on + strand, within PS417_03830 at 863.887 kb on - strand, within PS417_03830 at 864.093 kb on - strand, within PS417_03830 at 864.336 kb on - strand, within PS417_03830 at 864.360 kb on + strand, within PS417_03830 at 864.361 kb on - strand, within PS417_03830 at 864.465 kb on + strand, within PS417_03830 at 864.535 kb on - strand, within PS417_03830 at 864.760 kb on - strand, within PS417_03830 at 864.887 kb on + strand, within PS417_03830 at 864.887 kb on + strand, within PS417_03830 at 864.887 kb on + strand, within PS417_03830 at 864.904 kb on - strand, within PS417_03830 at 864.989 kb on - strand, within PS417_03830 at 865.353 kb on + strand, within PS417_03830 at 865.353 kb on + strand, within PS417_03830 at 865.354 kb on - strand, within PS417_03830 at 865.354 kb on - strand, within PS417_03830 at 865.377 kb on + strand, within PS417_03830 at 865.772 kb on - strand at 865.807 kb on + strand at 865.813 kb on - strand at 865.825 kb on - strand at 865.825 kb on - strand at 865.980 kb on + strand at 866.677 kb on + strand at 866.843 kb on - strand at 866.852 kb on + strand at 866.852 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 862,717 + PS417_03825 0.24 -0.2 862,718 - PS417_03825 0.24 +0.5 862,974 + PS417_03825 0.60 +0.4 862,974 + PS417_03825 0.60 +0.8 862,974 + PS417_03825 0.60 +1.9 862,975 - PS417_03825 0.60 -1.0 863,117 + PS417_03825 0.79 +0.3 863,117 + PS417_03825 0.79 +1.0 863,118 - PS417_03825 0.79 -1.5 863,118 - PS417_03825 0.79 -0.1 863,118 - PS417_03825 0.79 +0.3 863,118 - PS417_03825 0.79 -0.1 863,118 - PS417_03825 0.79 +1.9 863,118 - PS417_03825 0.79 -1.2 863,119 + PS417_03825 0.80 -0.3 863,119 + PS417_03825 0.80 -0.3 863,119 + PS417_03825 0.80 +2.2 863,120 - PS417_03825 0.80 +0.6 863,248 + -0.4 863,249 - +0.8 863,324 + -0.2 863,324 + +0.6 863,325 - -0.7 863,325 - -1.1 863,325 - -0.2 863,325 - -2.9 863,332 + +0.0 863,363 + -0.1 863,364 - -0.4 863,364 - +0.1 863,366 - -0.0 863,396 - +0.4 863,402 + -0.6 863,426 - -0.1 863,531 + +0.5 863,642 + -1.2 863,642 + -0.1 863,642 + +1.9 863,642 + -0.5 863,643 - -2.2 863,643 - -0.7 863,643 - +0.1 863,882 + PS417_03830 0.19 +0.1 863,886 + PS417_03830 0.19 +2.2 863,886 + PS417_03830 0.19 -0.3 863,887 - PS417_03830 0.19 -0.4 864,093 - PS417_03830 0.27 +0.5 864,336 - PS417_03830 0.37 -0.1 864,360 + PS417_03830 0.38 +2.5 864,361 - PS417_03830 0.38 -3.1 864,465 + PS417_03830 0.42 -1.0 864,535 - PS417_03830 0.44 -0.1 864,760 - PS417_03830 0.53 -0.5 864,887 + PS417_03830 0.58 +3.5 864,887 + PS417_03830 0.58 +0.0 864,887 + PS417_03830 0.58 +0.1 864,904 - PS417_03830 0.59 -0.1 864,989 - PS417_03830 0.62 +1.6 865,353 + PS417_03830 0.76 +0.1 865,353 + PS417_03830 0.76 +0.9 865,354 - PS417_03830 0.76 -0.7 865,354 - PS417_03830 0.76 -1.0 865,377 + PS417_03830 0.77 -0.3 865,772 - -0.3 865,807 + +0.4 865,813 - -1.8 865,825 - -0.9 865,825 - -0.9 865,980 + -1.0 866,677 + +0.1 866,843 - +0.1 866,852 + -0.2 866,852 + +1.5
Or see this region's nucleotide sequence