Experiment: L-Citrulline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_615 and Pf1N1B4_616 are separated by 69 nucleotides Pf1N1B4_616 and Pf1N1B4_617 are separated by 191 nucleotides
Pf1N1B4_615 - Arginine deiminase (EC 3.5.3.6), at 711,216 to 712,472
_615
Pf1N1B4_616 - Ornithine carbamoyltransferase (EC 2.1.3.3), at 712,542 to 713,552
_616
Pf1N1B4_617 - Carbamate kinase (EC 2.7.2.2), at 713,744 to 714,673
_617
Position (kb) 712
713
714 Strain fitness (log2 ratio) -2
-1
0
1
2 at 711.756 kb on + strand, within Pf1N1B4_615 at 711.757 kb on - strand, within Pf1N1B4_615 at 712.344 kb on + strand at 712.344 kb on + strand, within Pf1N1B4_615 at 712.345 kb on - strand, within Pf1N1B4_615 at 712.345 kb on - strand, within Pf1N1B4_615 at 712.345 kb on - strand, within Pf1N1B4_615 at 712.467 kb on + strand at 712.468 kb on - strand at 712.608 kb on + strand at 712.609 kb on - strand at 712.647 kb on + strand, within Pf1N1B4_616 at 712.648 kb on - strand, within Pf1N1B4_616 at 712.648 kb on - strand, within Pf1N1B4_616 at 712.770 kb on + strand, within Pf1N1B4_616 at 712.770 kb on + strand, within Pf1N1B4_616 at 712.771 kb on - strand, within Pf1N1B4_616 at 712.771 kb on - strand, within Pf1N1B4_616 at 713.550 kb on + strand at 713.575 kb on + strand at 713.576 kb on - strand at 713.744 kb on - strand at 713.933 kb on + strand, within Pf1N1B4_617 at 713.996 kb on + strand at 714.126 kb on - strand, within Pf1N1B4_617 at 714.126 kb on - strand at 714.315 kb on - strand, within Pf1N1B4_617 at 714.315 kb on - strand, within Pf1N1B4_617
Per-strain Table
Position Strand Gene LocusTag Fraction L-Citrulline (N) remove 711,756 + Pf1N1B4_615 0.43 -0.7 711,757 - Pf1N1B4_615 0.43 -1.0 712,344 + -0.0 712,344 + Pf1N1B4_615 0.90 -2.0 712,345 - Pf1N1B4_615 0.90 -1.3 712,345 - Pf1N1B4_615 0.90 +2.0 712,345 - Pf1N1B4_615 0.90 -0.8 712,467 + -0.3 712,468 - -0.0 712,608 + +0.7 712,609 - -0.0 712,647 + Pf1N1B4_616 0.10 -0.0 712,648 - Pf1N1B4_616 0.10 +1.3 712,648 - Pf1N1B4_616 0.10 +0.3 712,770 + Pf1N1B4_616 0.23 -0.0 712,770 + Pf1N1B4_616 0.23 -1.0 712,771 - Pf1N1B4_616 0.23 -0.3 712,771 - Pf1N1B4_616 0.23 -0.0 713,550 + -1.0 713,575 + -0.8 713,576 - +0.5 713,744 - -0.4 713,933 + Pf1N1B4_617 0.20 +1.0 713,996 + -0.0 714,126 - Pf1N1B4_617 0.41 -0.4 714,126 - -1.0 714,315 - Pf1N1B4_617 0.61 -0.0 714,315 - Pf1N1B4_617 0.61 -1.6
Or see this region's nucleotide sequence