Strain Fitness in Pseudomonas fluorescens SBW25 around PFLU_RS20310

Experiment: NAG (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS20300 and PFLU_RS20305 are separated by 109 nucleotidesPFLU_RS20305 and PFLU_RS20310 are separated by 30 nucleotidesPFLU_RS20310 and PFLU_RS20315 are separated by 60 nucleotidesPFLU_RS20315 and PFLU_RS20320 are separated by 17 nucleotides PFLU_RS20300: PFLU_RS20300 - phenylalanine--tRNA ligase subunit alpha, at 4,587,418 to 4,588,434 _RS20300 PFLU_RS20305: PFLU_RS20305 - 50S ribosomal protein L20, at 4,588,544 to 4,588,900 _RS20305 PFLU_RS20310: PFLU_RS20310 - 50S ribosomal protein L35, at 4,588,931 to 4,589,125 _RS20310 PFLU_RS20315: PFLU_RS20315 - translation initiation factor IF-3, at 4,589,186 to 4,589,719 _RS20315 PFLU_RS20320: PFLU_RS20320 - threonine--tRNA ligase, at 4,589,737 to 4,591,659 _RS20320 Position (kb) 4588 4589 4590Strain fitness (log2 ratio) -2 -1 0 1at 4588.457 kb on - strandat 4588.457 kb on - strandat 4588.489 kb on - strandat 4588.489 kb on - strandat 4588.489 kb on - strandat 4588.489 kb on - strandat 4588.546 kb on - strandat 4588.546 kb on - strandat 4588.546 kb on - strandat 4588.546 kb on - strandat 4588.562 kb on - strandat 4589.009 kb on - strand, within PFLU_RS20310at 4589.009 kb on - strand, within PFLU_RS20310at 4589.152 kb on - strandat 4589.152 kb on - strandat 4589.152 kb on - strandat 4589.152 kb on - strandat 4589.152 kb on - strandat 4589.158 kb on - strandat 4589.158 kb on - strandat 4589.158 kb on - strandat 4589.158 kb on - strandat 4589.188 kb on - strandat 4589.188 kb on - strandat 4589.188 kb on - strandat 4589.188 kb on - strandat 4589.188 kb on - strandat 4589.188 kb on - strandat 4589.188 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction NAG (C)
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4,588,457 - +1.2
4,588,457 - -1.4
4,588,489 - -1.1
4,588,489 - -0.9
4,588,489 - +0.7
4,588,489 - -0.3
4,588,546 - -0.9
4,588,546 - -0.3
4,588,546 - -0.7
4,588,546 - +0.4
4,588,562 - +0.3
4,589,009 - PFLU_RS20310 0.40 -0.3
4,589,009 - PFLU_RS20310 0.40 -2.2
4,589,152 - -1.3
4,589,152 - +0.4
4,589,152 - -0.3
4,589,152 - +0.2
4,589,152 - +0.9
4,589,158 - -0.8
4,589,158 - -1.3
4,589,158 - +0.2
4,589,158 - -0.9
4,589,188 - +0.3
4,589,188 - -1.1
4,589,188 - -1.2
4,589,188 - -0.7
4,589,188 - +0.3
4,589,188 - -0.8
4,589,188 - -0.6

Or see this region's nucleotide sequence