Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2829 and Shewana3_2830 are separated by 144 nucleotides Shewana3_2830 and Shewana3_2831 are separated by 49 nucleotides Shewana3_2831 and Shewana3_2832 are separated by 147 nucleotides Shewana3_2832 and Shewana3_2833 overlap by 1 nucleotides Shewana3_2833 and Shewana3_2834 are separated by 107 nucleotides
Shewana3_2829: Shewana3_2829 - GCN5-related N-acetyltransferase (RefSeq), at 3,377,964 to 3,378,602
_2829
Shewana3_2830: Shewana3_2830 - hypothetical protein (RefSeq), at 3,378,747 to 3,378,980
_2830
Shewana3_2831: Shewana3_2831 - hypothetical protein (RefSeq), at 3,379,030 to 3,379,428
_2831
Shewana3_2832: Shewana3_2832 - hypothetical protein (RefSeq), at 3,379,576 to 3,379,959
_2832
Shewana3_2833: Shewana3_2833 - hypothetical protein (RefSeq), at 3,379,959 to 3,380,732
_2833
Shewana3_2834: Shewana3_2834 - SecY interacting protein Syd (RefSeq), at 3,380,840 to 3,381,385
_2834
Position (kb)
3379
3380 Strain fitness (log2 ratio)
-2
-1
0
1 at 3378.934 kb on + strand, within Shewana3_2830 at 3378.991 kb on - strand at 3379.057 kb on - strand at 3379.071 kb on - strand, within Shewana3_2831 at 3379.105 kb on - strand, within Shewana3_2831 at 3379.251 kb on + strand, within Shewana3_2831 at 3379.259 kb on - strand, within Shewana3_2831 at 3379.365 kb on + strand, within Shewana3_2831 at 3379.615 kb on + strand, within Shewana3_2832 at 3379.640 kb on + strand, within Shewana3_2832 at 3379.827 kb on + strand, within Shewana3_2832 at 3379.954 kb on + strand at 3380.059 kb on + strand, within Shewana3_2833 at 3380.245 kb on + strand, within Shewana3_2833 at 3380.253 kb on - strand, within Shewana3_2833 at 3380.255 kb on + strand, within Shewana3_2833 at 3380.255 kb on + strand, within Shewana3_2833 at 3380.263 kb on - strand, within Shewana3_2833 at 3380.263 kb on - strand, within Shewana3_2833 at 3380.309 kb on - strand, within Shewana3_2833 at 3380.641 kb on + strand, within Shewana3_2833
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,378,934 + Shewana3_2830 0.80 -0.2 3,378,991 - -0.1 3,379,057 - -2.3 3,379,071 - Shewana3_2831 0.10 -0.8 3,379,105 - Shewana3_2831 0.19 -1.9 3,379,251 + Shewana3_2831 0.55 +1.4 3,379,259 - Shewana3_2831 0.57 -0.0 3,379,365 + Shewana3_2831 0.84 -0.2 3,379,615 + Shewana3_2832 0.10 -1.2 3,379,640 + Shewana3_2832 0.17 +1.4 3,379,827 + Shewana3_2832 0.65 -0.2 3,379,954 + -0.6 3,380,059 + Shewana3_2833 0.13 +1.4 3,380,245 + Shewana3_2833 0.37 -1.1 3,380,253 - Shewana3_2833 0.38 -0.7 3,380,255 + Shewana3_2833 0.38 -0.6 3,380,255 + Shewana3_2833 0.38 +1.4 3,380,263 - Shewana3_2833 0.39 -0.5 3,380,263 - Shewana3_2833 0.39 -1.4 3,380,309 - Shewana3_2833 0.45 -1.5 3,380,641 + Shewana3_2833 0.88 +0.2
Or see this region's nucleotide sequence