Strain Fitness in Shewanella sp. ANA-3 around Shewana3_1348

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShewana3_1347 and fliL are separated by 82 nucleotidesfliL and fliM are separated by 40 nucleotides Shewana3_1347: Shewana3_1347 - flagellar hook-length control protein (RefSeq), at 1,560,513 to 1,562,252 _1347 Shewana3_1348: fliL - flagellar basal body-associated protein FliL (RefSeq), at 1,562,335 to 1,562,859 fliL Shewana3_1349: fliM - flagellar motor switch protein FliM (RefSeq), at 1,562,900 to 1,563,931 fliM Position (kb) 1562 1563Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1561.470 kb on + strand, within Shewana3_1347at 1561.725 kb on - strand, within Shewana3_1347at 1561.727 kb on + strand, within Shewana3_1347at 1561.856 kb on + strand, within Shewana3_1347at 1562.002 kb on - strand, within Shewana3_1347at 1562.017 kb on + strand, within Shewana3_1347at 1562.468 kb on + strand, within fliLat 1562.705 kb on + strand, within fliLat 1563.175 kb on + strand, within fliMat 1563.241 kb on - strand, within fliMat 1563.608 kb on - strand, within fliMat 1563.635 kb on + strand, within fliMat 1563.692 kb on + strand, within fliM

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,561,470 + Shewana3_1347 0.55 -2.1
1,561,725 - Shewana3_1347 0.70 -0.0
1,561,727 + Shewana3_1347 0.70 -4.0
1,561,856 + Shewana3_1347 0.77 -0.4
1,562,002 - Shewana3_1347 0.86 +1.6
1,562,017 + Shewana3_1347 0.86 -4.1
1,562,468 + fliL Shewana3_1348 0.25 -3.9
1,562,705 + fliL Shewana3_1348 0.70 -2.2
1,563,175 + fliM Shewana3_1349 0.27 -1.9
1,563,241 - fliM Shewana3_1349 0.33 +1.6
1,563,608 - fliM Shewana3_1349 0.69 +1.6
1,563,635 + fliM Shewana3_1349 0.71 -5.3
1,563,692 + fliM Shewana3_1349 0.77 -3.5

Or see this region's nucleotide sequence