Strain Fitness in Shewanella sp. ANA-3 around Shewana3_1342

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShewana3_1340 and Shewana3_1341 are separated by 175 nucleotidesShewana3_1341 and fliF are separated by 44 nucleotidesfliF and fliG overlap by 8 nucleotides Shewana3_1340: Shewana3_1340 - two component, sigma54 specific, Fis family transcriptional regulator (RefSeq), at 1,553,017 to 1,554,357 _1340 Shewana3_1341: Shewana3_1341 - flagellar hook-basal body complex subunit FliE (RefSeq), at 1,554,533 to 1,554,871 _1341 Shewana3_1342: fliF - flagellar MS-ring protein (RefSeq), at 1,554,916 to 1,556,601 fliF Shewana3_1343: fliG - flagellar motor switch protein G (RefSeq), at 1,556,594 to 1,557,640 fliG Position (kb) 1554 1555 1556 1557Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1 2at 1554.068 kb on + strand, within Shewana3_1340at 1554.171 kb on + strand, within Shewana3_1340at 1554.171 kb on + strand, within Shewana3_1340at 1554.212 kb on - strand, within Shewana3_1340at 1554.302 kb on + strandat 1554.302 kb on + strandat 1554.308 kb on + strandat 1554.310 kb on - strandat 1554.417 kb on + strandat 1554.417 kb on + strandat 1554.417 kb on + strandat 1554.661 kb on - strand, within Shewana3_1341at 1554.810 kb on + strand, within Shewana3_1341at 1554.818 kb on - strand, within Shewana3_1341at 1554.856 kb on - strandat 1554.866 kb on + strandat 1554.953 kb on + strandat 1555.245 kb on + strand, within fliFat 1555.295 kb on + strand, within fliFat 1555.462 kb on + strand, within fliFat 1555.703 kb on + strand, within fliFat 1556.032 kb on - strand, within fliFat 1556.104 kb on + strand, within fliFat 1556.182 kb on - strand, within fliFat 1556.247 kb on + strand, within fliFat 1556.264 kb on + strand, within fliFat 1556.307 kb on - strand, within fliFat 1556.362 kb on - strand, within fliFat 1556.426 kb on + strand, within fliFat 1556.426 kb on + strand, within fliFat 1556.434 kb on - strandat 1556.455 kb on + strandat 1556.463 kb on - strandat 1556.463 kb on - strandat 1556.517 kb on + strandat 1556.592 kb on - strandat 1556.695 kb on - strandat 1556.947 kb on + strand, within fliGat 1557.112 kb on - strand, within fliGat 1557.127 kb on + strand, within fliGat 1557.128 kb on - strand, within fliGat 1557.225 kb on + strand, within fliGat 1557.238 kb on + strand, within fliG

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,554,068 + Shewana3_1340 0.78 +0.0
1,554,171 + Shewana3_1340 0.86 -0.7
1,554,171 + Shewana3_1340 0.86 +0.1
1,554,212 - Shewana3_1340 0.89 +1.6
1,554,302 + +0.3
1,554,302 + -2.5
1,554,308 + -2.1
1,554,310 - +0.0
1,554,417 + -1.3
1,554,417 + -0.7
1,554,417 + -0.5
1,554,661 - Shewana3_1341 0.38 +0.6
1,554,810 + Shewana3_1341 0.82 -3.6
1,554,818 - Shewana3_1341 0.84 +1.6
1,554,856 - +1.6
1,554,866 + +2.1
1,554,953 + -3.6
1,555,245 + fliF Shewana3_1342 0.20 -4.0
1,555,295 + fliF Shewana3_1342 0.22 -2.2
1,555,462 + fliF Shewana3_1342 0.32 -5.2
1,555,703 + fliF Shewana3_1342 0.47 -2.0
1,556,032 - fliF Shewana3_1342 0.66 +1.6
1,556,104 + fliF Shewana3_1342 0.70 -6.0
1,556,182 - fliF Shewana3_1342 0.75 +1.6
1,556,247 + fliF Shewana3_1342 0.79 -5.4
1,556,264 + fliF Shewana3_1342 0.80 -6.5
1,556,307 - fliF Shewana3_1342 0.83 +1.6
1,556,362 - fliF Shewana3_1342 0.86 +1.6
1,556,426 + fliF Shewana3_1342 0.90 -4.9
1,556,426 + fliF Shewana3_1342 0.90 -5.6
1,556,434 - +1.6
1,556,455 + -4.0
1,556,463 - +1.6
1,556,463 - +1.6
1,556,517 + -7.2
1,556,592 - +1.6
1,556,695 - +1.6
1,556,947 + fliG Shewana3_1343 0.34 -3.9
1,557,112 - fliG Shewana3_1343 0.49 +1.6
1,557,127 + fliG Shewana3_1343 0.51 -4.8
1,557,128 - fliG Shewana3_1343 0.51 +1.6
1,557,225 + fliG Shewana3_1343 0.60 -4.1
1,557,238 + fliG Shewana3_1343 0.62 -6.9

Or see this region's nucleotide sequence