Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1340 and Shewana3_1341 are separated by 175 nucleotides Shewana3_1341 and fliF are separated by 44 nucleotides fliF and fliG overlap by 8 nucleotides
Shewana3_1340: Shewana3_1340 - two component, sigma54 specific, Fis family transcriptional regulator (RefSeq), at 1,553,017 to 1,554,357
_1340
Shewana3_1341: Shewana3_1341 - flagellar hook-basal body complex subunit FliE (RefSeq), at 1,554,533 to 1,554,871
_1341
Shewana3_1342: fliF - flagellar MS-ring protein (RefSeq), at 1,554,916 to 1,556,601
fliF
Shewana3_1343: fliG - flagellar motor switch protein G (RefSeq), at 1,556,594 to 1,557,640
fliG
Position (kb)
1554
1555
1556
1557 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 1554.068 kb on + strand, within Shewana3_1340 at 1554.171 kb on + strand, within Shewana3_1340 at 1554.171 kb on + strand, within Shewana3_1340 at 1554.212 kb on - strand, within Shewana3_1340 at 1554.302 kb on + strand at 1554.302 kb on + strand at 1554.308 kb on + strand at 1554.310 kb on - strand at 1554.417 kb on + strand at 1554.417 kb on + strand at 1554.417 kb on + strand at 1554.661 kb on - strand, within Shewana3_1341 at 1554.810 kb on + strand, within Shewana3_1341 at 1554.818 kb on - strand, within Shewana3_1341 at 1554.856 kb on - strand at 1554.866 kb on + strand at 1554.953 kb on + strand at 1555.245 kb on + strand, within fliF at 1555.295 kb on + strand, within fliF at 1555.462 kb on + strand, within fliF at 1555.703 kb on + strand, within fliF at 1556.032 kb on - strand, within fliF at 1556.104 kb on + strand, within fliF at 1556.182 kb on - strand, within fliF at 1556.247 kb on + strand, within fliF at 1556.264 kb on + strand, within fliF at 1556.307 kb on - strand, within fliF at 1556.362 kb on - strand, within fliF at 1556.426 kb on + strand, within fliF at 1556.426 kb on + strand, within fliF at 1556.434 kb on - strand at 1556.455 kb on + strand at 1556.463 kb on - strand at 1556.463 kb on - strand at 1556.517 kb on + strand at 1556.592 kb on - strand at 1556.695 kb on - strand at 1556.947 kb on + strand, within fliG at 1557.112 kb on - strand, within fliG at 1557.127 kb on + strand, within fliG at 1557.128 kb on - strand, within fliG at 1557.225 kb on + strand, within fliG at 1557.238 kb on + strand, within fliG
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,554,068 + Shewana3_1340 0.78 +0.0 1,554,171 + Shewana3_1340 0.86 -0.7 1,554,171 + Shewana3_1340 0.86 +0.1 1,554,212 - Shewana3_1340 0.89 +1.6 1,554,302 + +0.3 1,554,302 + -2.5 1,554,308 + -2.1 1,554,310 - +0.0 1,554,417 + -1.3 1,554,417 + -0.7 1,554,417 + -0.5 1,554,661 - Shewana3_1341 0.38 +0.6 1,554,810 + Shewana3_1341 0.82 -3.6 1,554,818 - Shewana3_1341 0.84 +1.6 1,554,856 - +1.6 1,554,866 + +2.1 1,554,953 + -3.6 1,555,245 + fliF Shewana3_1342 0.20 -4.0 1,555,295 + fliF Shewana3_1342 0.22 -2.2 1,555,462 + fliF Shewana3_1342 0.32 -5.2 1,555,703 + fliF Shewana3_1342 0.47 -2.0 1,556,032 - fliF Shewana3_1342 0.66 +1.6 1,556,104 + fliF Shewana3_1342 0.70 -6.0 1,556,182 - fliF Shewana3_1342 0.75 +1.6 1,556,247 + fliF Shewana3_1342 0.79 -5.4 1,556,264 + fliF Shewana3_1342 0.80 -6.5 1,556,307 - fliF Shewana3_1342 0.83 +1.6 1,556,362 - fliF Shewana3_1342 0.86 +1.6 1,556,426 + fliF Shewana3_1342 0.90 -4.9 1,556,426 + fliF Shewana3_1342 0.90 -5.6 1,556,434 - +1.6 1,556,455 + -4.0 1,556,463 - +1.6 1,556,463 - +1.6 1,556,517 + -7.2 1,556,592 - +1.6 1,556,695 - +1.6 1,556,947 + fliG Shewana3_1343 0.34 -3.9 1,557,112 - fliG Shewana3_1343 0.49 +1.6 1,557,127 + fliG Shewana3_1343 0.51 -4.8 1,557,128 - fliG Shewana3_1343 0.51 +1.6 1,557,225 + fliG Shewana3_1343 0.60 -4.1 1,557,238 + fliG Shewana3_1343 0.62 -6.9
Or see this region's nucleotide sequence